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CN-118652930-B - Application of RcSAPK gene in regulation of drought resistance of plants and method for regulating drought resistance of plants

CN118652930BCN 118652930 BCN118652930 BCN 118652930BCN-118652930-B

Abstract

The invention provides an application of RcSAPK gene in regulating drought resistance of plants and a method for regulating drought resistance of plants. The first aspect of the invention provides an application of RcSAPK gene in regulating drought resistance of plants, wherein the RcSAPK gene is one of 1) to 3), 1) a nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO.1, 2) a nucleotide sequence derived from the nucleotide sequence shown as SEQ ID NO.1 through substitution, deletion or addition of one or more nucleotides, and 3) a nucleotide sequence with at least 80% identity with SEQ ID NO. 1. Experiments show that RcSAPK gene provided by the invention has an important function in drought stress resistance of plants, and provides an important basis for plant breeding.

Inventors

  • MING FENG
  • LIU MINGHUI
  • WANG BINGSHUANG
  • YANG BINAN
  • Li Yangqingxin

Assignees

  • 上海师范大学

Dates

Publication Date
20260512
Application Date
20240717

Claims (7)

  1. The application of the RcSAPK2 gene in improving the drought resistance of China rose is characterized in that the RcSAPK gene is a nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1.
  2. 2. The application of the RcSAPK gene in improving the drought resistance of China rose according to claim 1, which comprises the steps of introducing the RcSAPK gene into a starting plant, and enabling the RcSAPK gene to be over-expressed in the starting plant.
  3. 3. Use according to claim 2, characterized in that the RcSAPK gene is introduced into the starting plant, so that the RcSAPK gene is overexpressed in the starting plant, comprising in particular: Operably linking the open reading frame of the RcSAPK gene to a pCAMBIA2300 vector to form a pCAMBIA2300-SAPK2 recombinant vector containing the RcSAPK gene; the pCAMBIA2300-SAPK2 recombinant vector is introduced into agrobacterium, and a transgenic plant over-expressing RcSAPK genes is obtained by an agrobacterium-mediated method.
  4. 4. A method for improving drought resistance of China rose is characterized by comprising the steps of improving expression of RcSAPK genes in a starting plant; the RcSAPK gene is a nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1.
  5. 5. The method of claim 4, wherein increasing the expression of RcSAPK gene in the starting plant comprises introducing RcSAPK gene into the starting plant and over-expressing RcSAPK gene in the starting plant.
  6. 6. The method according to claim 5, wherein introducing RcSAPK gene into the starting plant, allowing the RcSAPK gene to be overexpressed in the starting plant, comprises: Operably linking the open reading frame of the RcSAPK gene to a pCAMBIA2300 vector to form a pCAMBIA2300-SAPK2 recombinant vector containing the RcSAPK gene; the pCAMBIA2300-SAPK2 recombinant vector is introduced into agrobacterium, and a transgenic plant over-expressing RcSAPK genes is obtained by an agrobacterium-mediated method.
  7. 7. Use of RcSAPK gene according to claim 1 in drought-resistant rose breeding.

Description

Application of RcSAPK gene in regulation of drought resistance of plants and method for regulating drought resistance of plants Technical Field The invention relates to the technical field of genetic engineering, in particular to application of RcSAPK gene in regulating drought resistance of plants and a method for regulating drought resistance of plants. Background In the context of global climate change, drought has become a key environmental challenge that restricts plant growth and development and geographic distribution. China rose (Rosa chinensis Jacq.) is used as a gardening plant which is widely applied and is popular with people, and the improvement of drought resistance has important significance for ecological adaptability and landscape application value. Therefore, how to regulate the drought resistance of China rose by means of genetic engineering is not only an important break for solving the current agricultural and gardening production problems, but also a basic research work for promoting the drought resistance breeding practice of plants. Disclosure of Invention The invention provides an application of RcSAPK gene in regulating drought resistance of plants and a method for regulating drought resistance of plants. The first aspect of the invention provides an application of RcSAPK gene in regulating drought resistance of plants, wherein the RcSAPK gene is one of 1) to 3): 1) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1; 2) The nucleotide sequence shown in SEQ ID NO.1 is derived by substitution, deletion or addition of one or several nucleotides; 3) A nucleotide sequence having at least 80% identity to SEQ ID No. 1. Further, the application of RcSAPK gene in regulating drought resistance of plant includes introducing RcSAPK gene to the plant to make RcSAPK gene over expressed in the plant. Further, the application of RcSAPK gene in regulating drought resistance of plant includes silencing RcSAPK gene expression in the original plant and reducing RcSAPK gene expression in the original plant. Further, introducing RcSAPK genes into the starting plants to enable the RcSAPK genes to be overexpressed in the starting plants, specifically comprising: Operably linking the open reading frame of the RcSAPK gene to a pCAMBIA2300 vector to form a pCAMBIA2300-SAPK2 recombinant vector containing the RcSAPK gene; the pCAMBIA2300-SAPK2 recombinant vector is introduced into agrobacterium, and a transgenic plant over-expressing RcSAPK genes is obtained by an agrobacterium-mediated method. Further, the plant is China rose. The second aspect of the invention provides a method for regulating drought resistance of plants, comprising regulating and controlling expression of RcSAPK genes in starting plants; The RcSAPK gene is one of the genes 1) to 3): 1) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1; 2) The nucleotide sequence shown in SEQ ID NO.1 is derived by substitution, deletion or addition of one or several nucleotides; 3) A nucleotide sequence having at least 80% identity to SEQ ID No. 1. Further, regulating RcSAPK gene expression in the starting plant includes introducing RcSAPK gene to the starting plant to over-express RcSAPK gene in the starting plant or silencing RcSAPK gene expression in the starting plant to reduce RcSAPK gene expression in the starting plant. Furthermore, rcSAPK genes are introduced into the starting plants, so that the RcSAPK genes are over-expressed in the starting plants, and transgenic plants with drought resistance superior to that of the starting plants are obtained. Further, introducing RcSAPK genes into the starting plants to enable the RcSAPK genes to be overexpressed in the starting plants, specifically comprising: Operably linking the open reading frame of the RcSAPK gene to a pCAMBIA2300 vector to form a pCAMBIA2300-SAPK2 recombinant vector containing the RcSAPK gene; the pCAMBIA2300-SAPK2 recombinant vector is introduced into agrobacterium, and a transgenic plant over-expressing RcSAPK genes is obtained by an agrobacterium-mediated method. The third aspect of the invention provides application of RcSAPK gene in drought-resistant breeding of plants. According to the invention, rcSAPK genes are cloned from the old China rose month powder, transient expression experiments prove that RcSAPK genes play an important role in response of China rose to dehydration pressure, silencing of RcSAPK genes causes the China rose to be more easily damaged under the dehydration pressure, and over-expression of RcSAPK genes is beneficial to resistance of China rose to the dehydration pressure. Therefore, the RcSAPK gene provided by the invention has an important function on drought stress resistance of China rose, and provides an important foundation for China rose breeding. Drawings FIG. 1 shows the relative expression levels of RcSAPK gene in different tissues of China rose 'YueYueFeng'; FIG. 2 is a phenotypic chart of the overexpress