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CN-118661636-B - Rapid collecting method of high-activity gleditsia sinensis pollen

CN118661636BCN 118661636 BCN118661636 BCN 118661636BCN-118661636-B

Abstract

The invention provides a rapid collecting method of high-activity gleditsia sinensis pollen, and relates to the technical field of pollen collection. The method comprises the steps of pollen dissolution, preliminary sieving, pressure filtration, water absorption drying, shaking, crushing, powdering and the like. The invention combines the pressurized filtration and the drying process to precisely control key process parameters, realizes the rapid collection of the high-activity gleditsia sinensis pollen, and has positive influence on the development of gleditsia sinensis breeding and gleditsia sinensis planting industry.

Inventors

  • ZHAO YANG
  • ZENG QINGYING
  • WANG XIURONG
  • LIU FUHUA
  • FU TIANYU

Assignees

  • 贵州大学

Dates

Publication Date
20260512
Application Date
20240717

Claims (10)

  1. 1. The rapid collecting method of the high-activity gleditsia sinensis pollen is characterized by comprising the following steps of: S1, pollen dissolution, namely collecting male inflorescences of gleditsia sinensis in the process of powder dispersion in the full bloom stage, putting into ultrapure water, slowly stirring, repeating for 3 times, and combining the solutions to obtain an aqueous solution A containing pollen; S2, primarily sieving, namely sieving the pollen-containing aqueous solution, and removing impurities to obtain a pollen aqueous solution B; S3, performing pressure filtration on the pollen water solution B to obtain pollen, wherein the pressure filtration is performed in a rubber piston cylinder with one end provided with a pollen isolation net, and the pressure of the pressure filtration is 5-6 kPa; S4, drying by water absorption, namely removing excessive moisture in pollen, performing primary drying at the temperature of 28 ℃ and the humidity of 60% -80% to obtain a solid pollen cake, properly dispersing the solid pollen cake, and performing secondary drying on the dispersed pollen at the temperature of 28 ℃ and the humidity of 30%; s5, vibrating, crushing and powdering, namely vibrating and crushing the dried pollen until the pollen is in a required powder shape, storing the pollen in a container, and storing the pollen under proper conditions to finish the rapid collection of the gleditsia sinensis pollen.
  2. 2. The method of claim 1, wherein the gleditsia sinensis is one or more of gleditsia sinensis (GLEDITSIA LINN) plant gleditsia sinensis (GLEDITSIA sinensislam.), gleditsia yunnanensis (Gleditsiajaponica var. Delavayi (frank.) l.c. li), gleditsia sinensis (Gleditsiajaponica lodd.ex w.baxter), gleditsia sinensis (GLEDITSIAFERA (lour.) merr.), gleditsia villosa (Gleditsiajaponica miq.var. Velutina l.c. li).
  3. 3. The method of claim 1, wherein the ratio of inflorescences to water/feed in S1 is 10-20 g/L.
  4. 4. The method of claim 1, wherein the stirring speed in S1 is 100-150 r/min.
  5. 5. The method of claim 1, wherein the sieving process in S2 is performed through a screen having a mesh size of 20-80 mesh.
  6. 6. The method according to claim 1, wherein the pressure filtration in S3 is performed in a rubber piston cylinder having a pollen isolation net mounted at one end, the rubber piston cylinder having a diameter of 10 to 20cm and a height of 30 to 60 cm, and the isolation net having a specification of 500 to 600 mesh.
  7. 7. The method according to claim 1, wherein the filtration is terminated after the pollen layer on the isolation net is 0.5-1.5 mm thick.
  8. 8. The method of claim 1, wherein the rubber piston cylinder is rocked at intervals of 6-8S during the pressurizing of S3.
  9. 9. The method of claim 1, wherein the primary drying time in S4 is 30min and the secondary drying time is 8 min.
  10. 10. The method according to claim 1, wherein the shaking and pulverizing is performed in a vortex oscillator with a frequency of 1000 to 1500 times/min and a shaking time of 1 to 3 min.

Description

Rapid collecting method of high-activity gleditsia sinensis pollen Technical Field The invention relates to the technical field of pollen collection, in particular to a rapid collection method of high-activity gleditsia sinensis pollen. Background The gleditsia sinensis is a special multifunctional ecological economic tree species in China, is widely used in the fields of forestation, greening, traditional medicines and the like, and has higher economic value. Because the gleditsia sinensis is a male amphoteric flower different plant, the cross is mainly used and the amphoteric flower is a functional female flower, and the natural pollination efficiency is lower, in the actual production, the problems of poor quality of the gleditsia sinensis, low yield of pods, uniform decline of seeds and the like exist, and the collection of gleditsia sinensis pollen for auxiliary pollination is an important way for improving the yield and quality of the pods. Gleditsia sinensis pollen is spherical or elliptic, and may have texture or protrusions on the surface, and is usually adhered to each other into small particles when scattered, and adhered to anther walls, petals and flower branches, so that pollen is difficult to collect. At present, the conventional method for collecting gleditsia sinensis pollen is to manually strip anthers, dry, collect and store the anthers, and the method has low efficiency, is time-consuming and labor-consuming, has extremely small collection amount, can not meet the requirements of modern rapid planting and research, and simultaneously, the pollen is easy to lose activity in the collection process. Therefore, the research and development of the method capable of rapidly collecting the gleditsia sinensis flower powder and guaranteeing the activity of the gleditsia sinensis flower powder has important significance to the development and scientific research of the gleditsia sinensis related industry. Disclosure of Invention In order to solve the problems, the invention provides a rapid collecting method of high-activity gleditsia sinensis pollen, which realizes the rapid collecting of the high-activity gleditsia sinensis pollen by combining the control of key parameters in each step through a specific pressurized filtration and water absorption drying process. The rapid collecting method of the high-activity gleditsia sinensis pollen comprises the following steps: S1, pollen dissolution, namely collecting male inflorescences of gleditsia sinensis in the process of powder dispersion in the full bloom stage, putting into ultrapure water, slowly stirring, repeating for 3 times, and combining the solutions to obtain an aqueous solution A containing pollen; S2, primarily sieving, namely sieving the pollen-containing aqueous solution, and removing impurities such as massive inflorescence residues, petals, broken anthers and the like to obtain a pollen aqueous solution B; s3, performing pressure filtration on the pollen water solution B to obtain pollen; S4, drying by water absorption, namely removing excessive moisture in pollen, performing primary drying at the temperature of 28 ℃ and the humidity of 60% -80% to obtain a solid pollen cake, properly dispersing the solid pollen cake, and performing secondary drying on the dispersed pollen at the temperature of 28 ℃ and the humidity of 30%; s5, vibrating, crushing and powdering, namely vibrating and crushing the dried pollen until the pollen is in a required powder shape, storing the pollen in a container, and storing the pollen under proper conditions to finish the rapid collection of the gleditsia sinensis pollen. Further, the gleditsia sinensis is one or more of gleditsia sinensis (GLEDITSIA LINN) of gleditsia (GLEDITSIA SINENSIS lam.), gleditsia sinensis (Gleditsiajaponica var. Delavayi (frank.) l.c. li), gleditsia sinensis (Gleditsiajaponica lod.ex w.baxter), gleditsia sinensis (GLEDITSIAFERA (lour.) merr.) and gleditsia sinensis (Gleditsiajaponica miq.var. Velutina l.c. li). Further, the inflorescences are collected at noon. Further, the ratio of inflorescences to water and liquid in the S1 is 10-20 g/L. Preferably, the ratio of inflorescence to water/liquid in S1 is 15g/L. Further, in the step S1, the stirring speed is 100-150 r/min. Preferably, the stirring speed in S1 is 120r/min. Further, the screen mesh in the step S2 is 20-80 meshes in specification. Preferably, the mesh size in S2 is 50 mesh. Further, in the step S3, the pressure filtration is performed in a rubber piston cylinder with a pollen isolation net installed at one end, the diameter of the rubber piston cylinder is 10-20 cm, the height of the rubber piston cylinder is 30-60 cm, and the specification of the isolation net is 500-600 meshes. Preferably, the diameter of the rubber piston cylinder is 15cm, and the height is 40cm. Preferably, when the gleditsia sinensis is gleditsia sinensis or gleditsia sinensis, the specification of the isolation net is 500 meshes, and when the gle