CN-118662575-B - Arisaema cum bile fermentation processing method based on regulation of oxygen content in fermentation environment

Abstract

The application relates to a Arisaema cum bile fermentation processing method based on regulating the oxygen content of a fermentation environment. Arisaema cum bile processed by the method. The application carries out fine control on the fermentation process of arisaema cum bile, and is mainly characterized in that the oxygen content in the environment is controlled in the fermentation process. The oxygen content in the fermentation environment is controlled by adding oxygen carrier and introducing preheated air. The toxicity of the arisaema cum bile is obviously reduced, and the tranquilizing effect is improved.

Inventors

• WEN BO
• SHENTU YINHONG
• NI SHANLIN
• ShenTu Hongfei
• WANG YANGYIN
• ZHOU LIHANG

Assignees

• 桐君谷生物医药科技(浙江)有限公司

Dates

Publication Date

20260512

Application Date

20240724

Claims (5)

1. 1. A fermentation processing method of arisaema cum bile, comprising the following preparation steps: Step 1), soaking and bleaching rhizoma arisaematis, and then taking out, and boiling together with ginger and alum until no dry core exists; step 2) taking out, airing or baking to be semi-dry, cutting into slices, and preprocessing the slices to obtain a pre-manufactured slice; Step 3) drying the prefabricated pieces, and then grinding the prefabricated pieces into prepared arisaema tuber powder; Step 4) adding purified water into the prepared arisaema tuber powder, bile and n-dodecane, and uniformly mixing to obtain a suspension; step 5) sterilizing the suspension obtained in the step 4) at high temperature; step 6), taking the sterilized suspension obtained in the step 5), and adding the composite fermentation fungus according to the mass percentage of 0.2% -1.0%; step 7), taking the suspension obtained in the step 6), keeping the temperature at 25-35 ℃ and the relative humidity at 65-90% under the stirring condition, and fermenting for 5 days, wherein the fermentation is completed; Step 8) taking the fermentation suspension obtained in the step 7), reducing the water content in a low-temperature evaporation mode to obtain a medicine mass with proper size, and then preparing a soft block with proper size; Step 9) taking the soft block obtained in the step 8), and drying in vacuum to volatilize n-dodecane in the soft block to obtain the final product arisaema cum bile, which is characterized in that, In the step 1), the mass ratio of the arisaema tuber, the ginger and the alum is 100:10:10 during the co-boiling; the pretreatment process in the step 2) comprises the steps of immersing a sheet in a cellulase solution for 5-10 min, then carrying out 200-300W ultrasonic wall breaking treatment for 10-15 min together with the enzyme solution, filtering out the sheet, and drying to obtain a prefabricated sheet, wherein the dosage of the cellulase is 200-300 u/1g of the sheet; The arisaema tuber powder, bile, n-dodecane and purified water are mixed according to the mass ratio of 100:200-300:5-30:100 in the step 4), wherein the bile is pig bile; the composite fermentation fungus in the step 6) consists of the Brevibacterium bifidum and the Pichia pastoris according to the mass ratio of 1:1; and 7) maintaining the oxygen content in the fermentation environment to be not lower than 15% by introducing preheated fresh air until the fermentation is finished.
2. 2. The processing method according to claim 1, wherein the soaking and bleaching step 1) is characterized in that the rhizoma arisaematis is placed in a washing and wetting pool and soaked in water for 2-3 days, water is changed for 2-3 times a day, alum accounting for 3wt% of the mass of the rhizoma arisaematis is added each time of water change, and the process is finished when the mouth and mouth taste have no tingling and tongue feeling.
3. 3. The processing method according to claim 1, wherein the drying temperature in the step 3) is 50 to 70 ℃ and the drying time is 3 to 6 hours.
4. 4. The processing method according to claim 1, wherein the high-temperature sterilization in step 5) is performed at a temperature of 110-121 ℃ for 15-35 min.
5. 5. The Arisaema cum bile prepared by the fermentation process according to any of claims 1-4.

Description

Arisaema cum bile fermentation processing method based on regulation of oxygen content in fermentation environment Technical Field The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a Arisaema cum bile fermentation processing method based on adjusting the oxygen content of a fermentation environment. Background Arisaema cum bile is a traditional starter with long history of application, is prepared from fine powder of arisaema tuber and bile of cattle, sheep or pig, or is prepared from fine powder of arisaema tuber and bile of cattle, sheep or pig by fermentation, has bitter taste, slight pungency and cool nature, and has effects of clearing heat, resolving phlegm, calming endogenous wind and arresting convulsion. The pharmacopoeia of 2020 describes that Arisaema cum bile is square or cylindrical. Brown, grey-brown or brown-black, hard, slightly fishy smell, and bitter taste. The powder was pale yellow brown. Parenchyma cells are round and filled with gelatinized starch grains. The length of the calcium oxalate needle crystal beam is 20-90 mu m. The diameters of the threaded conduit and the annular conduit are 8-60 mu m. The bile arisaema is firstly carried in Song Dynasty of the infant medicine syndrome Zhengzhi, wherein the bile is dried in the shade for hundred days in the preserved and brewed cattle. The same period of "Shengji Su Ji" is carried by "the cattle gallbladder is immersed in three-stage baking, and the cattle gallbladder is dried by a sudden drying when one volt is boiled". The long-time negative dry method for brewing the ox gall and the exposure method after the ox gall is boiled, although the preparation time and the drying mode are different, both have the description that the arisaema tuber powder is brewed or soaked in the ox gall for a long time, and can be regarded as an origin of the arisaema tuber method for preparing the ox gall by the later-time fermentation. The first method for preparing arisaema bile and cake in Taiping Huimin He Ji Fang is "decoction washing, baking, grinding into powder, using oxgall and cake, baking, and it is proposed to pretreat arisaema without long-time fermentation, thus improving production efficiency. The method for preparing arisaema cum bile by mixing the arisaema tuber fine powder with bile is widely used at present, and thus the rudiment can be seen. "Sheep gall" is first mentioned in "Puji Ben Fang". As can be seen, in Song dynasty, there is a non-uniformity of the fermentation process, and the use of cow gall or sheep gall during the processing of arisaema cum bile. After Song dynasty, the Dannan star preparation methods are used, developed and innovated. Of 17 Arisaema cum bile processing historic data in the period from Yuan to Min, 12 are long-time yin dry methods of brewing ox gall, and are classified as fermentation methods. The fermentation process in the first and the second stages is basically the same as that in the Song dynasty, the operation flow is described in more detail, and the idea that the quality of arisaema cum bile can be improved by prolonging the fermentation time and increasing the adding times of oxgall appears. "Water-break, use Buffalo gall in winter and summer". The Danxi heart method is carried out by using the bull gall of yellow rice and the arisaema tuber of Chinese yam, repairing the arisaema tuber in hand, airing the dried in the air and taking the Chinese yam every year. The cattle gallbladder should be good for three or four times. The Ming Dynasty, ben Cao gang mu, describes "making nan xing method, in which the arisaema is ground into powder, and the Bulleyan is taken from the yellow bull bile, and the regulating agent is taken into the gallbladder, which is a dry place in the wind, and is good for the elderly. More complex methods for processing Arisaema cum bile occur in the late stage. Dannan xing is marked by "Jiu Zhong" in the book of Zhu Long Shu from Zhu Yue. The Qing dynasty ' young integration ' records the preparation method of the nine-made ox gall arisaema in more detail, namely ' taking one ox gall, pouring bile into a bowl, mixing and evenly loading arisaema powder into the gall skin, suspending the arisaema powder in the place of the gall skin for drying in the shade, cutting the front gall into pieces when the bile exists, loading the arisaema powder into the suspension with bile and evenly, and can be used for loading the nine-gall powder into the Zhibao real bezoar. " The arisaema cum bile prepared by nine-year is characterized by using 9 fel bovis seu Bubali, large bile consumption, complex preparation and remarkable drug effect, but the time required for preparation is recorded differently, and the people who take two or three years are said to be the best people who take brown to nine years and who succeed nine years. The exposure method after the ox gall is boiled may be changed into a method of mixing and fermenting the