CN-118685250-B - Microbial sample pretreatment system

CN118685250BCN 118685250 BCN118685250 BCN 118685250BCN-118685250-B

Abstract

The application relates to the technical field of microbial sample treatment, and discloses a microbial sample pretreatment system. The microbial sample pretreatment system comprises a workbench, a sample treatment assembly and a collection assembly, wherein the workbench is used for limiting a first area, a second area and a third area which are arranged side by side, the sample treatment assembly is located in the first area and comprises a sample loading device and a sample transferring device, the sample loading device is used for placing a sample container for containing samples, the sample transferring device is used for transferring the sample container in the first area and the second area, the collection assembly is located in the second area and comprises a culture dish loading device, a glass slide loading device, an inoculating device and a drawing and smearing device, the inoculating device and the drawing and smearing device are used for drawing and/or smearing glass slides on the culture dish, and the collection assembly is located in the third area and comprises a culture dish collection device and a glass slide collection device, the culture dish collection device is used for collecting the drawn culture dish, and the glass slide collection device is used for collecting the glass slides after smearing.

Inventors

JIA HONGTAO
TIAN MAOCHANG
FAN HUI
LIU FENGHAI
MA XUEYAN
LIU RUNDONG
DONG QINGKUN
WANG YAN
LIU HAIQUAN
ZHOU BIN
GUO PEICHEN

Assignees

青岛海尔生物医疗股份有限公司

Dates

Publication Date: 20260508
Application Date: 20240603

Claims (17)

1. A microbial sample pretreatment system, comprising: a table defining a first region, a second region, and a third region disposed side-by-side; The sample processing assembly is positioned in the first area and comprises a sample loading device and a sample transferring device, the sample loading device is used for placing a sample container for containing a sample, and the sample transferring device is used for transferring the sample container in the first area and the second area; the scribing and smearing assembly is positioned in the second area and comprises a culture dish loading device, a slide loading device, an inoculating device and a scribing and smearing device, wherein the inoculating device and the scribing and smearing device are used for scribing the culture dish and/or smearing the slide; the sample collection assembly is positioned in a third area and comprises a culture dish sample collection device and a glass slide sample collection device, wherein the culture dish sample collection device is used for collecting a culture dish after scribing, and the glass slide sample collection device is used for collecting a glass slide after smearing; The inoculating device comprises a workbench, a rotating frame, an inoculating loop, a first operating station, a second operating station, a boss, a first magnetic piece and a first magnetic matching piece, wherein the inoculating loop can rotate at a first position and a second position relative to the rotating frame, when the inoculating loop is positioned at the first position, a first included angle exists between the inoculating loop and the vertical direction, and the head of the inoculating loop can correspond to the first operating station; when the inoculating loop is positioned at the second position, a second included angle exists between the inoculating loop and the vertical direction, the head of the inoculating loop corresponds to the second operation station, wherein the second included angle is larger than the first included angle, the second operation station is an inoculating station, and the scribing smear device is arranged at the inoculating station; The boss is positioned at one side of the rotating frame and corresponds to the inoculation station, the boss protrudes out of the circumferential outer wall surface of the rotating frame, and when the inoculation ring rotates around the axial direction of the rotating frame and moves towards the inoculation station, the boss contacts with the inoculation ring and lifts the head of the inoculation ring so as to enable the inoculation ring to move from a first position to a second position; The first magnetic matching piece is arranged at the tail part of the inoculating loop and corresponds to the inoculating station, when the inoculating loop moves to the inoculating station and rotates to the second position, the first magnetic piece corresponds to the first magnetic matching piece, and repulsive magnetic force is formed between the first magnetic piece and the first magnetic matching piece so as to apply downward force to the head part of the inoculating loop.
2. The microbial sample pretreatment system of claim 1, wherein the streak smear device comprises: The tray is movably arranged at one side of the inoculation device, can be matched with the culture dish loading device or the glass slide loading device and is used for bearing the culture dish or the glass slide; Wherein, the upper wall surface of the tray is configured with a culture dish loading groove and a slide loading groove, the culture dish loading groove is used for loading the culture dish, and the slide loading groove is used for loading the slide.
3. The microbial sample pretreatment system of claim 2, wherein the tray comprises: A first reinforcing rib, the upper wall surface part of which is recessed to form a slide loading groove; the second reinforcing ribs are arranged in a crossing manner with the first reinforcing ribs and are connected with the middle parts of the first reinforcing ribs; the third reinforcing rib is connected with one end of the first reinforcing rib; the fourth reinforcing rib is connected with the other end of the first reinforcing rib; wherein the second reinforcing rib, the third reinforcing rib and the fourth reinforcing rib define a culture dish loading groove.
4. A microbial sample pretreatment system according to claim 3, wherein, The third reinforcing rib, the second reinforcing rib and the fourth reinforcing rib are arranged at intervals along the length direction of the first reinforcing rib, so that clamping grooves are formed between the adjacent reinforcing ribs, and/or, The tray further includes: And the positioning needle is convexly arranged at the outer side of the culture dish loading groove, and is used for positioning the culture dish when the culture dish is loaded in the culture dish loading groove.
5. The microbial sample pretreatment system of claim 1, wherein the slide loading device comprises: the sample discharging device is arranged on the workbench in a lifting manner and is provided with first storage grids arranged side by side along the vertical direction, and the first storage grids are used for placing glass slides; The first push rod is arranged at one side of the sample discharging device and is used for pushing the glass slide of the first storage lattice out of the first storage lattice; The scribing and smearing device is connected with the workbench in a sliding way, and can move to the other side of the sample outlet device along the slide way so as to bear the slide glass pushed out from the first storage grid.
6. The microbial sample pretreatment system of claim 1, wherein the culture dish loading device comprises: the operation platform is rotationally arranged on the workbench; The storage bins are arranged above the operation platform at intervals along the circumferential direction of the operation platform, extend along the vertical direction and are used for placing culture dishes; the lifting mechanism is arranged at the bottom of the storage bin and used for lifting the culture dish; The clamping jaw assembly is arranged on one side of the operation platform and comprises clamping jaws and a lifting rotating mechanism, the clamping jaws are positioned above the storage bin and used for clamping the culture dish and driving the culture dish to move.
7. A microbial sample pretreatment system according to claim 6, wherein, The lifting mechanism penetrates through the operating platform, when the lifting mechanism ascends to the upper side of the operating platform, the lifting mechanism and the operating platform are limited, the operating platform is limited to rotate relative to the workbench, and when the lifting mechanism descends to the lower side of the operating platform, the lifting mechanism and the operating platform are released from being limited, and the operating platform can rotate relative to the workbench; and/or the number of the groups of groups, Culture dish loading attachment still includes: The position sensor is used for detecting the position of the culture dish in the storage bin, the position sensor is electrically connected with the clamping jaw assembly, and when the lifting mechanism drives the culture dish to rise to a preset position, the clamping jaw assembly clamps the culture dish at the preset position.
8. The microbial sample pretreatment system of claim 1, wherein the sample treatment assembly further comprises: the liquid adding grabbing device comprises a liquid adding device and a sample clamp which are connected, wherein the sample clamp is used for transferring a sample container and/or opening and closing a cover body of the sample container, and the liquid adding device is used for adding liquid into the sample container.
9. The microbial sample pretreatment system of claim 8, wherein the sample holder comprises: The guide rail assembly comprises an X-axis guide rail, a Y-axis guide rail and a Z-axis guide rail; The mechanical arm is movably arranged on the Z-axis guide rail; the mechanical arm clamping jaw is arranged at the lower end part of the mechanical arm and is used for clamping a cover body of the sampling sample container and/or the switching sample tube; wherein, liquid feeding device locates one side of arm, and liquid feeding device is connected with the arm including liquid storage bottle, the liquid feeding pump that is linked together and liquid feeding needle to the liquid feeding needle can be along arm motion to subtend sample container liquid feeding.
10. The microbial sample pretreatment system of claim 8, wherein the sample treatment assembly further comprises: The vibration device is arranged at one side of the liquid adding grabbing device, the vibration device is provided with a vibration groove which is used for placing a sample container, the vibration groove can be matched with the liquid adding grabbing device to open and close a cover or add liquid and can be used for vibrating the solution in the sample container, and/or, The sample processing assembly further comprises: and the heating device is arranged on one side of the oscillating device and is used for heating the sample in the sample container.
11. The microbial sample pretreatment system of claim 8, further comprising: the receiving device comprises a receiving cover body, the receiving cover body is movably arranged on the workbench and is positioned at one side of the sample transferring device; After the sample container is transferred into the sample transfer device and the cover is opened, the receiving cover body can move to the lower part of the liquid adding and grabbing device and is positioned below the sample container cover body clamped by the liquid adding and grabbing device and used for receiving liquid dropped from the sample container cover body.
12. The microbial sample pretreatment system of claim 1, wherein the sample collection assembly comprises: the grabbing device comprises grabbing clamping claws which are movably arranged in the third area and between the second area and the third area and used for grabbing a culture dish or a glass slide and transferring the culture dish or the glass slide; The printing device is arranged corresponding to the culture dish sample receiving device and is used for printing the label and pasting the label on the culture dish; the code spraying device is arranged corresponding to the slide glass collecting device and is used for spraying codes to the slide glass.
13. The microbial sample pretreatment system of claim 12, wherein the culture dish sample collection device comprises: the sample collection bin is used for collecting the labeled culture dishes; One end of the slide rail is communicated with the sample collection bin, the other end of the slide rail corresponds to the printing device, and the grabbing device can grab the culture dish on the slide rail so that the culture dish moves into the sample collection bin along the slide rail; and the turnover mechanism is arranged on the sliding rail and used for turning over the culture dish on the sliding rail so that the culture dish is reversely buckled and stored in the sample collection bin.
14. The microbial sample pretreatment system of claim 12, wherein the culture dish sample collection device comprises: The sample collection platform is rotationally arranged on the workbench; the sample collection bin is arranged on the sample collection platform and extends along the vertical direction, and defines a plurality of storage spaces for placing labeled culture dishes; the jacking mechanism is arranged at the bottom of the storage space and used for lifting the culture dish in the storage space; The stop mechanism is movably arranged at the bottom opening of the storage space; When the lifting mechanism drives the culture dish to rise to a preset position, the stop mechanism moves to the sixth position, the stop mechanism shields the bottom opening of the storage space and is abutted to the lower wall surface of the culture dish at the lowest layer, and the downward movement of the culture dish is avoided.
15. The microbial sample pretreatment system of claim 12, wherein the printing device comprises: a printer; The labeling platform is arranged at one side of the printer and used for bearing a culture dish to be labeled; The labeling mechanism is arranged at one side of the printer and is used for labeling the culture dish of the labeling platform; wherein, when grabbing device drives the culture dish and removes to subsides mark platform top, grabbing device fixes the culture dish lid, and the preset distance of culture dish box body downstream increases the surface height of the lateral wall of culture dish box body to the labeller constructs the lateral wall to the culture dish box body and pastes the mark.
16. The microbial sample pretreatment system of claim 15, wherein the grasping device comprises: A mobile device; The grabbing mechanical arm is connected with the mobile device and can move between the second area and the third area along with the mobile device and in the third area, and the grabbing clamping jaw is positioned at the lower end of the grabbing mechanical arm; The first grabbing clamping jaw is located at the outer side of the second grabbing clamping jaw, the length of the first grabbing clamping jaw is larger than that of the second grabbing clamping jaw, when the grabbing device grabs a culture dish, the second grabbing clamping jaw is adsorbed above the culture dish cover body, the first grabbing clamping jaw is clamped on the circumferential outer side wall of the culture dish box body to drive the culture dish to be transferred, and when the grabbing device drives the culture dish to move to the upper side of the labeling platform, the first grabbing clamping jaw is loosened, and the second grabbing clamping jaw adsorbs the culture dish cover body to enable the culture dish box body to move downwards by a preset distance under the action of gravity.
17. The microbial sample pretreatment system of any of claims 1 to 16, wherein the slide collection device comprises: the mobile platform is movably arranged on the workbench and used for placing the smeared glass slide; the sample collection device is arranged on the workbench in a lifting manner, the moving platform can move to one side of the sample collection device, the sample collection device is provided with a plurality of second storage grids arranged along the vertical direction, and the second storage grids are used for placing smeared glass slides; the second push rod is positioned at one side of the sample receiving device and is used for pushing the glass slide of the movable platform into the second storage grid.

Description

Microbial sample pretreatment system Technical Field The application relates to the technical field of microbial sample processing components, for example to a microbial sample pretreatment system. Background At present, in the field of pretreatment of clinical microbiological examination, the existing operation is generally manual operation, and the operations of sample pretreatment, sample uncapping, sample extraction, culture dish inoculation scribing, slide smearing, sample capping and the like are carried out, so that the problems of high labor cost, low efficiency and high operation risk are solved. The utility model discloses a microorganism sample pretreatment system, which is provided with a workbench, be equipped with test tube transport station on the workstation in proper order, culture dish marking off station and the culture dish transport station of perpendicular distribution with test tube transport station, the adjacent side of test tube transport station is equipped with test tube dish and test tube fixed station of uncapping, the adjacent side of test tube dish is equipped with untreated culture dish storehouse position station in proper order, label prints and pastes station and treated culture dish storehouse position station, test tube transport station includes first actuating mechanism and electronic rotatory clamping jaw, culture dish marking off station is equipped with second actuating mechanism, marking off device and streak inoculation subassembly, culture dish transport station includes the fixed plate, locate the lift drive subassembly of fixed plate upper end and locate the culture dish clamping jaw of fixed plate lower extreme, untreated culture dish storehouse position station is equipped with untreated culture dish storehouse position carousel. In the process of implementing the embodiments of the present disclosure, it is found that at least the following problems exist in the related art: In the related art, since a plurality of automation devices are required to be arranged in the microbial sample pretreatment system, the cost of the microbial sample pretreatment system is high. The microbial sample pretreatment system in the related art can only be used for scribing the culture dish, and has the advantages of single function and low cost performance. It should be noted that the information disclosed in the above background section is only for enhancing understanding of the background of the application and thus may include information that does not form the prior art that is already known to those of ordinary skill in the art. Disclosure of Invention The following presents a simplified summary in order to provide a basic understanding of some aspects of the disclosed embodiments. This summary is not an extensive overview, and is intended to neither identify key/critical elements nor delineate the scope of such embodiments, but is intended as a prelude to the more detailed description that follows. The embodiment of the disclosure provides a microbial sample pretreatment system, which is used for improving the functional diversity of the microbial sample pretreatment system and improving the cost performance of the microbial sample pretreatment system. The embodiment of the disclosure provides a microbial sample pretreatment system, which comprises a workbench, a sample treatment assembly, a scribing and smear assembly and a sample collection assembly, wherein the workbench is used for limiting a first area, a second area and a third area which are arranged side by side, the sample treatment assembly is positioned in the first area and comprises a sample loading device and a sample transferring device, the sample loading device is used for placing a sample container for containing samples, the sample transferring device is used for transferring the sample container in the first area and the second area, the scribing and smear assembly is positioned in the second area and comprises a culture dish loading device, a glass slide loading device, an inoculation device and a scribing and smear device, the inoculation device and the scribing and smear device are used for scribing and/or smearing a culture dish, and the sample collection assembly is positioned in the third area and comprises a culture dish collecting device and a glass slide collecting device, the culture dish collecting device is used for collecting the scribed culture dish, and the glass slide collecting and the smeared glass. The microbial sample pretreatment system provided by the embodiment of the disclosure can realize the following technical effects: The workbench defines a first area, a second area and a third area which are arranged side by side, the sample processing assembly, the scribing smear assembly and the sample collecting assembly are respectively positioned in the first area, the second area and the third area, so that a user can conveniently operate and observe the processing condition of a s