CN-118697935-B - Method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine and application

CN118697935BCN 118697935 BCN118697935 BCN 118697935BCN-118697935-B

Abstract

The invention discloses a method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine and application thereof, wherein the method comprises the steps of stirring and dissolving a riboflavin suspension, a guanosine suspension and a sodium persulfate mother solution in a casein solution to obtain a mixed solution, and then irradiating the mixed solution by light to ensure gelation to obtain the casein hydrogel, wherein the concentration of the casein solution is 5-15% w/v, the concentration of the riboflavin suspension is 1-5mM, the concentration of the guanosine suspension is 0.02-0.04mM, and the concentration of the sodium persulfate mother solution is 45-150mM. The application is the use of casein hydrogels in wound dressings. The casein hydrogel prepared by the invention has good antibacterial effect and proper mechanical property, and can be used as a wound material without affecting wound recovery due to compression.

Inventors

YAO KAIYONG
REN DAXI
TANG YANHUA
CHEN QIYI
ZHU QINCHAO
XIAO YINGPING
LIU BOJING

Assignees

浙江大学

Dates

Publication Date: 20260508
Application Date: 20240531

Claims (3)

1. The method for preparing casein hydrogel by crosslinking and mediating guanosine of riboflavin is characterized in that a mixed solution is obtained by stirring and dissolving a riboflavin suspension, a guanosine suspension and a sodium persulfate mother solution in a casein solution, and then the mixed solution is irradiated by illumination to ensure gelation, thus obtaining the casein hydrogel, wherein: the concentration of the casein solution is 10% w/v; The concentration of the riboflavin suspension was 3 mM; The concentration of guanosine suspension is 0.035 mM; The concentration of the sodium persulfate mother liquor is 75 mM; The wavelength of the illumination is 450 nm, the illumination time is 3min, and the illumination intensity is 1.5 mW cm -2 ; the illumination position is arranged at the position 1 cm above the mixed solution.
2. The method for preparing casein hydrogel through crosslinking and mediating guanosine of claim 1, wherein the casein solution is prepared by adding casein to NaOH solution of 0.1 mol/L, and then stirring 18: 18h on a magnetic stirrer.
3. Use of a casein hydrogel prepared according to the method of claim 1 or 2 in the preparation of a wound dressing.

Description

Method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine and application Technical Field The invention relates to the technical field of hydrogel preparation, in particular to a method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine and application thereof. Background In recent years, casein hydrogels have been further used. Casein gel has been a good material for forming functional dairy products in the food industry for decades. Casein is suitable for film preparation because it readily forms a stable polymer network by cross-linking with other materials. Casein is a multifunctional protein derived from milk, which forms a colloid having a specific viscosity in water due to its excellent water retention, emulsifying property, and stability. Like general protein hydrogel, casein hydrogel has the advantages of good biocompatibility, biodegradability and low toxicity. In addition, the casein hydrogel takes casein as a main component, and is a material which is easy to obtain and low in cost. In general, casein hydrogels are well-established in the food industry, but they have not yet demonstrated ideal application in biomedical related fields. Disclosure of Invention The invention aims to provide a method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine and application thereof. The casein hydrogel prepared by the invention has good antibacterial effect and proper mechanical property, and can be used as a wound material without affecting wound recovery due to compression. The technical scheme provided by the invention is that the method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine comprises the steps of stirring and dissolving a riboflavin suspension, a guanosine suspension and a sodium persulfate mother solution in a casein solution to obtain a mixed solution, and then irradiating the mixed solution with light to ensure gelation to obtain the casein hydrogel, wherein: The concentration of the casein solution is 5-15% w/v; the concentration of the riboflavin suspension is 1-5mM; the concentration of the guanosine suspension is 0.02-0.04mM; The concentration of the sodium persulfate mother liquor is 45-150mM. The method for preparing casein hydrogel by crosslinking and mediating guanosine by using the riboflavin has the concentration of 10% w/v. The preparation method of casein hydrogel by crosslinking riboflavin and mediating guanosine comprises the steps of adding casein into 0.1mol/L NaOH solution, and then placing the solution on a magnetic stirrer for stirring for 18 hours. The method for preparing casein hydrogel by crosslinking and mediating guanosine of the riboflavin suspension has a concentration of 3mM. The method for preparing casein hydrogel by crosslinking and mediating guanosine by using riboflavin, wherein the concentration of the guanosine suspension is 0.035mM. The aforementioned method for preparing casein hydrogel by crosslinking riboflavin and mediating guanosine, wherein the concentration of the sodium persulfate mother liquor is 75mM. The method for preparing casein hydrogel by crosslinking and mediating guanosine by using the riboflavin has the wavelength of 450nm, the irradiation time period of 3min and the irradiation intensity of 1.5 mW cm -2. The method for preparing casein hydrogel by crosslinking and mediating guanosine by using the riboflavin is characterized in that the illumination position is arranged at a position 1cm above the mixed solution. The application of the casein hydrogel prepared by the method, and the casein hydrogel is used in wound dressing. Compared with the prior art, the invention uses the riboflavin as a cross-linking agent and sodium persulfate as a catalyst, prepares the casein hydrogel through illumination, and adds guanosine to mediate the casein hydrogel into the hydrogel in the preparation process of the gel. In the preparation process of the casein hydrogel, the casein-based hydrogel is crosslinked through the reaction between the riboflavin and tyrosine residues by utilizing the irradiation treatment, and meanwhile, the guanosine reacts with the riboflavin to generate hydrogen peroxide, so that the casein hydrogel has a good antibacterial effect, has proper mechanical properties, and can be used as a wound material without pressing the wound material to influence wound recovery. Drawings FIG. 1 is a casein hydrogel at 5mM riboflavin concentration; FIG. 2is a casein hydrogel at a riboflavin concentration of 3 mM; FIG. 3 shows a graph of storage modulus versus loss modulus for casein gels at different concentrations of SPS; FIG. 4 is a graph showing the hydrogen peroxide production of gels of varying guanosine content; FIG. 5 is a schematic representation of the hydrogen peroxide yield of the gel at various illumination times; FIG. 6 is a schematic representation of the change in swelling mass of hydrogels; FIG. 7 is a gra