CN-118724249-B - Microalgae filter bed coupled microbial fuel cell system and sewage treatment and ARGs propagation control method thereof

CN118724249BCN 118724249 BCN118724249 BCN 118724249BCN-118724249-B

Abstract

The application relates to the technical field of water treatment, and particularly discloses a microalgae filter bed coupled microbial fuel cell system and a method for treating sewage and controlling ARGs propagation. A microalgae filter bed coupling microbial fuel cell system comprises a body, an anaerobic zone, an aerobic zone and an aerobic zone, wherein the anaerobic zone is arranged on the body and comprises anode electrodes which are sequentially arranged, the anode electrodes are arranged on one side of a first active carbon layer, anode microalgae are inoculated on the anode electrodes, the aerobic zone is arranged on the body, the aerobic zone and the aerobic zone are sequentially arranged along the height direction of the body, the aerobic zone comprises cathode electrodes which are sequentially arranged, the cathode electrodes are arranged on one side of a second active carbon layer, the cathode electrodes are inoculated with cathode microalgae, the cathode electrodes are electrically connected with the anode electrodes, and eukaryotic microalgae are selected for both the anode microalgae and the cathode microalgae. The system of the application can be used for water treatment and has the advantages of simple structure, flexible operation, rapid starting, no aeration, stable operation, low cost and the like.

Inventors

SONG HAILIANG
Yue Zhixuan
HE XIWEI
WANG HUIXIANG
CHEN ZIMO
YANG YULI
PENG QIWEI
YANG XIAOLI

Assignees

南京师范大学

Dates

Publication Date: 20260512
Application Date: 20240621

Claims (5)

1. A method for treating antibiotic sewage and controlling ARGs transmission by a microalgae filter bed coupled microbial fuel cell system, The microalgae filter bed coupled microbial fuel cell system comprises: A body; the anaerobic zone is arranged in the body, and the anaerobic zone comprises the following components in sequence: a first activated carbon layer (3), the first activated carbon layer (3) being for filtration; an anode electrode (4), wherein the anode electrode (4) is arranged on one side of the first active carbon layer (3), and the anode electrode (4) is inoculated with anode microalgae (5); The aerobic zone is arranged in the body and comprises the following components in sequence: A second activated carbon layer (7), the second activated carbon layer (7) being for filtration; The cathode electrode (8) is arranged on one side of the second active carbon layer (7), the cathode electrode (8) is inoculated with cathode microalgae (9), and the cathode electrode (8) is electrically connected with the anode electrode (4); Eukaryotic microalgae are selected as the anode microalgae (5) and the cathode microalgae (9); the anodic microalgae (5) are chlorella, and the domestication condition of the anodic microalgae (5) is cultivation under dark weak light; the cathode microalgae (9) are chlorella or clinopodium, and the domestication condition of the cathode microalgae (9) is 12h illumination and 12h photophobia; The body further comprises a supporting layer (2) and a transition layer (6), wherein the supporting layer (2), the anaerobic zone, the transition layer (6) and the aerobic zone are sequentially arranged from bottom to top along the height direction of the body; The supporting layer (2) is filled with gravel with the particle size of 6-8mm, the transition layer (6) is filled with iron ore with the particle size of 2-6mm, and the method is characterized by comprising the following steps: s1, before use, inoculating anaerobic sludge and anode microalgae (5) on the anode electrode (4) in the anaerobic zone, and inoculating cathode microalgae (9) in logarithmic growth phase on the cathode electrode (8) in the aerobic zone; s2, water treatment, namely pumping the sewage containing the antibiotics from the bottom of the body, sequentially treating the sewage by an anaerobic zone and an aerobic zone, and finally discharging the sewage after overflowing from the top of the body.
2. The method for treating antibiotic wastewater and controlling ARGs propagation by using a microalgae filter bed coupled microbial fuel cell system according to claim 1, wherein the first activated carbon layer (3) and the second activated carbon layer (7) are respectively filled with activated carbon, and the particle size of the activated carbon is 1-2mm.
3. The method for treating antibiotic wastewater and controlling ARGs propagation in a microalgae filter bed coupled microbial fuel cell system according to claim 1, wherein the anode electrode (4) and the cathode electrode (8) comprise two clamping layers and a hydrophilic graphite carbon felt, and the hydrophilic graphite carbon felt is arranged between the two clamping layers.
4. The method for treating antibiotic wastewater and controlling ARGs propagation by using the microalgae filter bed coupled microbial fuel cell system according to claim 1, wherein the body is provided with a water inlet pipe (1) and a water outlet pipe (12), the water inlet pipe (1) is positioned at one end of the body close to the supporting layer (2), and the water outlet pipe (12) is positioned at one end of the body close to the aerobic zone.
5. The method for treating antibiotic wastewater and controlling ARGs propagation in a microalgae filter bed coupled microbial fuel cell system according to claim 1, wherein the hydraulic retention time is 48h in step S2.

Description

Microalgae filter bed coupled microbial fuel cell system and sewage treatment and ARGs propagation control method thereof Technical Field The application relates to the technical field of water treatment, in particular to a microalgae filter bed coupled microbial fuel cell system and a method for treating sewage and controlling ARGs transmission. Background Antibiotics are widely used in the medical and aquaculture industries, and at lower residual concentrations, antibiotics still affect biological functions, resulting in chronic poisoning effects in aquatic organisms. At the same time, antibiotic residues induce the production of antibiotic-resistant bacteria (Antibiotic Resistance Bacteria, ARB) and antibiotic resistance genes (Antibiotic RESISTANCE GENES, ARGS). ARGs has the characteristics of horizontal transfer and difficult biodegradation, can spread in soil, water and other mediums, even spread along with food chains, and can cause great threat to ecological environment and human health. The traditional ecological filter bed is a low-cost ecological sewage treatment process widely applied in industries such as aquaculture, livestock and poultry farming and the like. Microbial fuel cells (Microbial Fuel Cell, MFC) are a novel energy and wastewater purification technology that oxidizes organic and inorganic substances with microorganisms as catalysts while generating electricity. By filling the carbonaceous material with conductive properties, the ecological filter bed can be evolved into a microbial fuel cell in a short-circuit state, and can generate electric energy while obtaining efficient pollutant treatment, thereby having certain advantages in removing antibiotics and controlling ARGs compared with the common wetland. However, researches show that ARGs exists in the effluent of the coupling system, and meanwhile, the oxygen source of the cathode is naturally dissolved oxygen or artificially oxygenated, and the oxidation-reduction gradient of the anode and the cathode is limited, so that improvement of the coupling system is urgently needed. Disclosure of Invention In order to improve the removing effect of the coupling system on ARGs, the application provides a microalgae filter bed coupled microbial fuel cell system and application. The application provides a microalgae filter bed coupled microbial fuel cell system, which adopts the following technical scheme: In a first aspect, the present application provides a microalgae filter bed coupled microbial fuel cell system, comprising: A body; Anaerobic zone, the anaerobic zone is located the body, the anaerobic zone is including setting up in order: a first activated carbon layer for filtration; the anode electrode is arranged on one side of the first active carbon layer, and is inoculated with anode microalgae; the aerobic zone is arranged on the body, the aerobic zone and the aerobic zone are sequentially arranged along the height direction of the body, and the aerobic zone comprises sequentially arranged: a second activated carbon layer for filtration; The cathode electrode is arranged on one side of the second active carbon layer, the cathode electrode is inoculated with cathode microalgae, and the cathode electrode is electrically connected with the anode electrode; eukaryotic microalgae are selected as the anode microalgae and the cathode microalgae. By adopting the technical scheme, the application utilizes the redox gradient to be matched with the microbial fuel cell, and simultaneously introduces microalgae into the aerobic zone and the anaerobic zone to form a algae symbiotic system, so that various action mechanisms such as ecology, biology, electrochemistry, bioelectrochemistry and the like are fully utilized to strengthen the antibiotic removal and ARGs control. In the application, microalgae are preferably inoculated on both the anode electrode and the cathode electrode, and have various removing mechanisms such as adsorption, accumulation, biodegradation, photodegradation, hydrolysis and the like on antibiotics. And because of the phylogenetic distance between eukaryotes and prokaryotes, eukaryotic microalgae are a natural barrier, which can effectively control ARGs transfer between symbiotic bacteria, thereby reducing the abundance of ARGs in the wastewater treatment process matrix. Meanwhile, the microalgae can transfer electrons to the anode through an electron mediator in the anode of the MFC and provide a substrate for the anode, and can generate oxygen in the cathode of the MFC as a cathode electron acceptor. Optionally, the anodic microalgae are chlorella, and the domestication condition of the anodic microalgae is culturing under dark weak light. By adopting the technical scheme, the anodic microalgae can provide electron donors with bacteria in a dark environment, so that a algae symbiotic system is formed, the bacteria and the microalgae exchange various nutrient elements in a coexisting period, and secretion signal subst