CN-118745471-B - Molecular marker related to sheep feed conversion rate and application of molecular marker in breeding

Abstract

The invention provides a molecular marker related to sheep feed conversion rate and application thereof in breeding. According to the invention, through PCR amplification and sequence analysis of sheep APOD genes, a T/C polymorphic site exists at the 233 st position of an amplified fragment, the AQP primer pair is further used for detecting the polymorphic site of 897 Hu sheep, a least squares model is built, the correlation analysis of genotype and feed conversion rate is carried out, and finally, the amplified APOD gene fragment can be used as a molecular marker related to sheep feed conversion rate. The molecular marker can be used for cultivating new varieties of grain-saving high-quality mutton sheep, provides a genetic engineering means for genetic improvement of sheep feed conversion rate, and has great practical application value.

Inventors

• WANG WEIMIN
• XIAO ZIYUE
• TIAN HUIBIN
• ZHANG YUKUN
• ZHAO YUAN
• XU DAN

Assignees

• 兰州大学

Dates

Publication Date

20260512

Application Date

20240718

Claims (5)

1. 1. The application of the primer pair for detecting the molecular marker related to the feed conversion rate of the Hu sheep is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, wherein Y at 233bp represents T or C, the mutation leads to the T/C polymorphism of the molecular marker, the feed conversion rate of the sheep carrying the TT genotype is obviously lower than that of the sheep carrying the TC genotype, and the breeding is the breeding of grain-saving Hu sheep.
2. 2. The application of the primer pair for detecting the molecular marker related to the feed conversion rate of the Hu sheep in the breeding of the Hu sheep is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, wherein Y at 233bp represents T or C, the mutation leads to the T/C polymorphism of the molecular marker, the feed conversion rate of the sheep carrying the TT genotype is obviously lower than that of the sheep carrying the TC genotype, the breeding is used for breeding grain-saving Hu sheep, and the nucleotide sequence of the primer pair is shown as SEQ ID NO.2 and SEQ ID NO. 3.
3. 3. The application of the AQP SNP primer pair for detecting the molecular marker related to the feed conversion rate of the Hu sheep in the breeding of the Hu sheep is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, Y at 233bp represents T or C, the mutation leads to T/C polymorphism of the molecular marker, the feed conversion rate of the sheep carrying TT genotype is obviously lower than that of the sheep carrying TC genotype, the breeding is used for breeding grain-saving Hu sheep, the AQP SNP primer pair comprises a forward primer for detecting AlleleT, a forward primer for detecting AlleleC and a universal reverse primer, the nucleotide sequence of the forward primer for detecting AlleleT is shown as SEQ ID NO.4, the nucleotide sequence of the forward primer for detecting AlleleC is shown as SEQ ID NO.5, and the nucleotide sequence of the universal reverse primer is shown as SEQ ID NO. 6.
4. 4. The application of the detection kit for detecting molecular markers related to the feed conversion rate of the Hu sheep in the breeding of the Hu sheep is characterized in that the nucleotide sequence of the molecular markers is shown as SEQ ID NO.1, wherein Y at 233bp represents T or C, the mutation leads to T/C polymorphism of the molecular markers, the feed conversion rate of sheep carrying TT genotype is obviously lower than that of sheep carrying TC genotype, the breeding is breeding of grain-saving Hu sheep, and the detection kit comprises a primer pair or an AQP primer pair, wherein the nucleotide sequence of the primer pair is shown as SEQ ID NO.2 and SEQ ID NO. 3; the nucleotide sequences of the AQP primer pair are shown as SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO. 6.
5. 5. The application of the method for detecting the molecular marker related to the feed conversion rate of the Hu sheep in the detection of the feed conversion rate of the Hu sheep is characterized in that the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, wherein Y at 233bp represents T or C, and the detection method comprises the following steps: s1, amplifying Hu sheep genome DNA by using a primer pair shown as SEQ ID NO.2 and SEQ ID NO.3 or an AQP primer pair shown as SEQ ID NO. 4-6; s2, carrying out typing identification on the site of 233bp of the molecular marker of the amplified product obtained in the step S1; The feed conversion rate of the sheep carrying the TT genotype is obviously lower than that of the sheep carrying the TC genotype.

Description

Molecular marker related to sheep feed conversion rate and application of molecular marker in breeding Technical Field The invention belongs to the technical field of screening and application of molecular markers, and particularly relates to an APOD gene fragment serving as a molecular marker for influencing sheep feed conversion rate and application of the molecular marker in breeding. Background Mutton is one of the main meat food materials in the current society, and improving the economic benefit of mutton has become one of the most important tasks. In sheep production, the cost of feed is relatively high, so that the improvement of the feed conversion rate of sheep has important research significance. The index designed by genetic improvement of feed investment is reduced on the premise of not influencing the normal growth of animals, and the feed utilization rate of livestock and poultry is reduced. The feed utilization rate of animals refers to the utilization rate of the fed feed, and is mainly influenced by two factors of diet and animals. The feed efficiency (FEED EFFICIENCY, FE) is the short term of the feed conversion rate (Feed conversion ration, FCR), also called feed return, and the feed conversion rate generally refers to the amount of feed consumed by animals with weight gain of 1kg, namely the feed weight ratio (FEED INTAKE/gain, F/G), and is an important economic index for measuring the feed utilization rate for long time. In addition, the weight gain feed ratio (gain/FEED INTAKE, G/F) is an index (Lancaster P A,Carstens G E,Jr C D,et al.Phenotypic and genetic relationships of residual feed intake with performance and ultrasound carcass traits in Brangus heifers.Journal of Animal Science,2009,87(12):3887-3896), for representing the relationship between livestock and poultry weight gain and daily ration feed intake, the feed conversion rate is widely applied at home and abroad, the feed ratio is used in meat product production, and the feed ratio is used in poultry egg production. The (AggreyS E,Karnuah AB,Sebastian B,et al.Genetic properties of feed efficiency parameters in meat-type chickens.Genetics Selection Evolution,2010,42(1):1-5.AggreyS E,Rekaya R.Dissection of Koch's residual feed intake:implications for selection.Poultry Science,2013,92(92):2600-2605). researches on improvement of the feed conversion rate start from the aspects of increasing the weight gain of livestock and poultry or the yield of meat and eggs and reducing the feed consumption show that the genetic transmission of the feed conversion rate is 0.26-0.41, belongs to medium genetic traits, is controlled by heredity, can select and match sheep flocks according to scientific data through selecting improvement (Willems O W,MillerS P,Wood B J.Assessment of residual body weight gain and residual intake and body weight gain as feed efficiency traits in the turkey(Meleagris gallopavo).Genetics Selection Evolution,2013,45(1):1-8)., and is one of the feasible methods (Mount Tao, research on the production performance and the body composition of different RFI fattening lambs and digestion metabolism, gansu agricultural university, 2016). How to determine the scientific basis is one of the problems to be solved. Currently, most of the indicators are analyzed based on animal phenotypes, with few systematic analysis of sheep feed conversion rates at the genetic level. Apolipoprotein D (ApoD), found for the first time in human plasma (Jarrier et al, 1963), was initially identified as a component of high density lipoprotein, was first isolated from human plasma in 1973 (Mcconathy et al, 1973), and was subsequently demonstrated to be a member of the lipocalin family, and ApoD was able to bind cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid (RASSART ET al., 2000). Furthermore, unlike other apolipoproteins, which are synthesized mainly in the liver and intestinal tract, apolipoprotein D is widely expressed in various tissues such as the pancreas, kidney, placenta, spleen and brain (Drayna et al 1986;Drayna et al, 1986;Ganfornina et al,2008;S e guin et al, 1995). In recent years, more and more experimental studies indicate that APOD plays an important role in the growth, development and differentiation of embryos and adults, the generation, development and apoptosis of tumor cells, the resistance to external environmental stress, the maintenance of body lipid homeostasis, and the generation, damage repair and disorders and diseases of some nervous systems. Apolipoproteins are involved in the transport and metabolism of lipid materials in the animal body and maintain lipid balance, so that they have a certain relationship with various cardiovascular diseases associated with lipids. For the APOD gene, it has been determined to be involved in lipoprotein metabolism in both vertebrate and human studies (Cong Yi, 2008). Studies have shown that high APOD in the ligament adipose pool in women with obesity is associat