CN-118765701-B - Application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings

Abstract

The invention relates to the technical field of agricultural application, in particular to application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings. The invention discovers and proves the application of 3, 5-dihydroxytoluene in improving the low temperature and low oxygen resistance of tobacco seedlings and can promote the growth of tobacco seedlings. Meanwhile, the exogenous 3, 5-dihydroxytoluene treatment is easy to operate in practice, effective, and expected to become a good method for improving the capability of resisting low-temperature hypoxia stress in the production practice of tobacco floating seedling raising, and has great application potential.

Inventors

• GUAN YAJING
• LI LONGXI
• HU JIN
• NIU YONGZHI
• SUO WENLONG
• ZHENG YUNYE

Assignees

• 浙江大学

Dates

Publication Date

20260512

Application Date

20240812

Claims (3)

1. The application of 1.3,5-dihydroxytoluene in improving the low temperature and low oxygen resistance of tobacco seedlings is characterized in that, The concentration of the 3, 5-dihydroxytoluene is [3 mmol/L,8 mmol/L ]; the application mode of the 3, 5-dihydroxytoluene is that the 3, 5-dihydroxytoluene is added into the water culture solution of 2 leaves of the tobacco seedlings in the 1-heart stage, and the root systems of the tobacco seedlings are ensured to be fully immersed in the water culture solution.
2. 2. The use according to claim 1, wherein the tobacco seedlings are cultivated by sterilizing tobacco seeds, rinsing with distilled water, draining the water, accelerating germination at 25 ℃, and then cultivating until the 2-leaf 1-heart stage.
3. 3. The use according to claim 2, wherein the germination is carried out by 16 h light treatments followed by 8 h dark treatments in one day, repeated for 7-8 days.

Description

Application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings Technical Field The invention relates to the technical field of agricultural application, in particular to application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings. Background As widely planted leaf crops, low-temperature stress is one of common abiotic stress in tobacco production, photosynthesis and organic matter accumulation of leaves are easily inhibited, the total nitrogen content of the leaves is increased, and the total phosphorus, total potassium, reducing sugar and nicotine content are reduced, so that the quality of tobacco leaves is affected. Meanwhile, in the tobacco floating seedling production process, low temperature and low oxygen stress exist simultaneously, so that tobacco seedling growth is blocked and even death is easy to occur, and the activity of a seedling plant is reduced, thereby seriously affecting tobacco production. Phenolic acids are a class of organic acids containing a phenolic ring, formed by carbohydrate metabolism, and are widely found in many plants as secondary metabolites. Currently, phenolic acids have been shown to play a number of important roles in plant protection against abiotic stress. It can help plants adapt to various adverse environments by regulating plant growth, removing active oxygen in vivo, resisting oxidation, influencing gene expression and metabolic accumulation and other mechanisms. Exogenous treatment is an effective means of helping plants resist abiotic stress and can enhance stress resistance of plants. In agricultural production, exogenous application of phenolic acids has been shown to help plants resist abiotic stress. 3, 5-dihydroxytoluene has been widely used as a phenolic acid substance in the fields of chemistry and medicine as an organic synthesis raw material, an analytical reagent, a pharmaceutical intermediate, and the like. These applications show that it has good chemical stability and biological activity, providing a basis for its potential application in plant stress resistance. Disclosure of Invention The invention provides an application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides an application of 3, 5-dihydroxytoluene in improving low temperature and low oxygen resistance of tobacco seedlings. According to the application, 3, 5-dihydroxytoluene is treated on tobacco seedlings subjected to low-temperature hypoxia stress, and as a result, the fact that 3, 5-dihydroxytoluene is a phenolic acid substance can induce the tobacco seedlings to resist low-temperature hypoxia composite stress, and the damage of the low-temperature hypoxia stress to the tobacco seedlings can be relieved. The 3, 5-dihydroxytoluene treatment has a remarkable promotion effect on tobacco seedling growth under low-temperature and low-oxygen conditions, and the peroxidase activity, the superoxide dismutase activity, the catalase activity, the ascorbate peroxidase activity, the soluble sugar content and the soluble protein content are remarkably improved compared with the control group without the 3, 5-dihydroxytoluene treatment, and the malondialdehyde content is remarkably reduced compared with the control group without the 3, 5-dihydroxytoluene treatment. In addition, the root length and the relative chlorophyll content of the tobacco seedlings treated by the 3, 5-dihydroxytoluene are obviously increased. Increasing root length helps to increase the plant's efficiency of water and nutrient uptake into the soil, which is critical to maintaining normal growth under adverse conditions, while increasing chlorophyll content helps to maintain or increase photosynthesis levels, ensuring that the plant is able to acquire sufficient energy source under adverse conditions. Thus, it can be deduced that 3, 5-dihydroxytoluene can be used as a potential regulator to improve the viability and growth performance of tobacco seedlings under low oxygen and low temperature conditions by improving the nutrition absorption capacity and photosynthesis efficiency of the tobacco seedlings. Here, the 3, 5-dihydroxytoluene is not limited to the addition in the form of a formulated solution/formulation or a solid powder. In conclusion, the inventor discovers that 3, 5-dihydroxytoluene promotes the growth of tobacco seedlings under low-temperature and low-oxygen stress, and provides a new application of the 3, 5-dihydroxytoluene. Preferably, the concentration of the 3, 5-dihydroxytoluene is (0, 8 mmol/L). When the 3, 5-dihydroxytoluene is added in the form of a formulated solution/preparation, the concentration of 3, 5-dihydroxytoluene in the culture broth after the addition can be controlled to be (0, 8 mmol/L.) when the 3, 5-dihydroxyto