CN-118773238-B - QTL for controlling corn kernel dehydration rate and application thereof

Abstract

The invention relates to a main effect QTL for controlling the dehydration rate of corn kernels, a related molecular marker and application thereof in screening or improving the moisture content or the dehydration rate of the corn kernels, and belongs to the field of molecular genetics. The invention provides a main effect QTL-qKDR 1 for controlling the dehydration rate of corn kernels, and simultaneously discloses 9 InDel loci which are obviously related to the dehydration rate of the corn kernels. The invention discloses a method for screening corn kernel dehydration rate based on molecular markers developed on Indel sites. Further, methods of manipulating qKDR a1 using genetic engineering means to alter the moisture content or dehydration rate of corn kernels are disclosed.

Inventors

• YAN JIANBING
• YU YANHUI
• LI WENQIANG
• LIU YUANFANG
• XU JIETING
• ZHUO LIN

Assignees

• 华中农业大学
• 湖北洪山实验室
• 常州新米生物科技有限公司

Dates

Publication Date

20260508

Application Date

20240430

Priority Date

20240125

Claims (6)

1. 1. Use of a maize genomic fragment for improving the moisture content or dehydration rate trait of maize kernels, wherein the fragment position corresponds to B73 genome V4 version Chromosome 1: 20,007,756 to 20,009,147; The nucleotide sequence or reverse complementary sequence of the fragment is shown as any one of SEQ ID NO. 1 or SEQ ID NO. 2.
2. 2. A method for increasing the moisture content of corn kernels or reducing the dehydration rate, characterized in that a genomic fragment corresponding to between 501 and 1272 bases corresponding to SEQ ID No. 2 in the corn material to be modified is deleted and a plant with increased moisture content or reduced dehydration rate of corn kernels is selected.
3. 3. The method of claim 2, wherein the deletion of the fragment is performed by gene editing.
4. 4. The method of claim 3, wherein the target DNA sequence for gene editing is shown in SEQ ID NO.3 and SEQ ID NO. 4.
5. 5. A kit for increasing the moisture content of corn kernels or reducing the dehydration rate is characterized by comprising any one of the following: (1) An RNA molecule capable of recognizing the target sequence of claim 4; (2) A DNA molecule encoding the RNA of (1); (3) A vector expressing the RNA of (1); The sequences of the RNA molecules are shown as SEQ ID NO.5 and SEQ ID NO. 6.
6. 6. Use of the method of any one of claims 2 to 4 or the kit of claim 5 for improving the moisture content or dehydration rate profile of corn kernels.

Description

QTL for controlling corn kernel dehydration rate and application thereof Technical Field The invention relates to a main effect QTL for controlling the dehydration rate of corn kernels, a related molecular marker and application thereof in screening or improving the moisture content or the dehydration rate of the corn kernels, and belongs to the field of molecular genetics. Background Grain moisture is a key factor affecting the mechanical harvest quality, safe storage and economic benefits of corn. The moisture content of the seeds during harvesting has great influence on corn harvesting, drying, storage, transportation and processing utilization, and too high moisture content often causes corn growers and operators to suffer economic loss, reduces economic benefit, and also easily causes the mildewing of the seeds to influence the corn quality. In addition, the grain harvesting is one of main factors limiting the production of corns in China, and the most critical link of the corn grain harvesting is that the moisture content of corn grains can not reach the standard moisture content (Wang Z,Wang X,Zhang L,Liu X,Di H,Li T,Jin X.QTL underlying field grain drying rate after physiological maturity in maize(Zea Mays L.)[J].Euphytica,2012,185(3):521-528.). which can be less than or equal to 25% of the grain harvesting during harvesting, so the corn grain harvesting is very important for breeding corn varieties with low grain moisture content during harvesting. In addition, the low water content of the seeds can shorten the growth period of corn, which has great production significance for harvesting the corn before the frost period in the high latitude area of China and not affecting the wheat planting in the Huang-Huai-Hai area. On the other hand, harvesting purposes are different, and suitable harvesting moisture is also different. For example, corn kernels for silage purposes require kernels with high moisture content, typically above 30%, and in the case of fresh corn, up to 75% moisture content for good mouthfeel and nutritional value. Therefore, QTLs or functional genes capable of controlling corn kernel moisture content and dehydration rate are of great industrial value. Disclosure of Invention In order to solve the problems, the invention positions a main effect QTL-qKDR 1 for controlling the moisture content and the dehydration rate of corn kernels through map cloning, identifies 10 molecular markers in a section, can screen the moisture content or the dehydration rate of the corn kernels by utilizing the qKDR1 section and the molecular markers, and can also operate qKDR1 by utilizing a genetic engineering means to change the moisture content or the dehydration rate of the corn kernels. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention provides the use of a maize genomic fragment for improving the moisture content or dehydration rate trait of maize kernels, wherein the fragment position corresponds to B73 genome V4 version Chromosome 1:20,007,756 to 20,009,147; in some embodiments, the nucleotide sequence or reverse complement of the above fragment is as shown in any one of SEQ ID NO.1 or SEQ ID NO. 2. The invention also provides a molecular marker, which is characterized in that the marker corresponds to the 67 th, or 70 th, or 473 th, or 513 th, or 597 th, or 599 th, or 605 th, or 722-779 th, or 729-778 th, or 1131-1377 th of the sequence shown in SEQ ID NO. 2. The invention also provides a method for identifying or assisting in identifying the moisture content or dehydration rate characteristics of corn kernels, which is characterized by comprising the following steps of (1) detecting the molecular markers in the materials to be detected, (2) if the detection result is that the markers are contained, the materials to be detected show the low moisture content or the rapid dehydration rate characteristics of the kernels, and if the detection result is that the markers are not contained, the materials to be detected show the high moisture content or the slow dehydration rate characteristics of the kernels; the invention also provides a method for cultivating the corn material with low grain water content or rapid dehydration rate, which is characterized in that the nucleic acid molecule with the sequence shown as SEQ ID NO.2 is introduced into the corn material to be improved, the molecular marker in the material to be detected is detected according to the method, and the material containing the molecular marker is screened. The invention also provides a method for increasing the moisture content of corn kernels or reducing the dehydration rate, which is characterized in that a genome segment corresponding to between 501 and 1272 bases or 1049 and 1417 bases corresponding to SEQ ID NO.2 in corn materials to be improved is deleted, and plants with the corn kernels with increased moisture content or reduced dehydration rate are selected. In some em