CN-118834908-B - Application of gene GhWRKY46 in regulation and control of plant growth and development

Abstract

The invention belongs to the technical field of bioengineering, and relates to application of a gene GhWRKY46 in regulating and controlling plant growth and development. The invention provides application of a gene GhWRKY46 in regulating plant growth and development. The GhWRKY46 gene is cloned from upland cotton, and the over-expression transgenic strain obtained by constructing an over-expression vector and heterologously expressing in Arabidopsis is advanced compared with wild flowering, so that the GhWRKY46 gene plays an important role in controlling cotton flowering period. Provides important application value for cotton variety improvement and breeding.

Inventors

• WANG HANTAO
• SUN MENGXI
• MA LIANG
• FU XIAOKANG
• YU SHUXUN
• WEI HENGLING
• KANG MENG
• LU JIANHUA

Assignees

• 中国农业科学院棉花研究所

Dates

Publication Date

20260508

Application Date

20240822

Claims (1)

1. 1. The application of the over-expression gene GhWRKY46 in the flowering period of plants in advance is characterized in that the open reading frame sequence of the gene GhWRKY46 is shown as SEQ ID NO.1, and the amino acid sequence encoded by the gene GhWRKY46 is shown as SEQ ID NO. 2; The plant is Arabidopsis thaliana.

Description

Application of gene GhWRKY46 in regulation and control of plant growth and development Technical Field The application belongs to the technical field of bioengineering, and particularly relates to application of a gene GhWRKY46 in regulating and controlling plant growth and development. Background The WRKY transcription factor family is a class of plant-specific transcription factors that have 75 members in the model plant arabidopsis. Since this class of transcription factors contains a highly conserved WRKYGQK amino acid sequence at the N-terminus, it is designated as a WRKY transcription factor. WRKY transcriptional factors regulate a variety of plant developmental processes including dormancy, germination, morphogenesis, flowering, gametophyte and seed development, and the like. In recent years, there have been more reports on WRKY transcription factors in terms of regulation of flowering time of plants. For example, atWRKY34 binds to the promoter of CUL3A, activates its expression, and a large amount of accumulated CUL3A interacts with FRI to degrade FRI, thereby inhibiting FLC expression, participating in vernalization pathway-mediated plant flowering (Hu et al, 2014), atWRKY71 and AtWRKY75 directly bind to the W-box motif on the FT promoter to activate FT expression, accelerating flowering (Yu et al, 2016; zhang et al, 2018), atWRKY12 and AtWRKY13 participate in gibberellin pathway regulation flowering through interaction with DELLAs protein under short-day conditions, and at the same time, their expression patterns have very close relation with plant age, the expression level of WRKY13 gradually decreases with plant age, and the expression level of WRKY12 steadily increases (Li et al, 2016). WRKY63 plays a dual role in flowering regulation. It inhibits flowering by activation AtFLC under non-vernalizing conditions, but promotes flowering during vernalization by activating the inhibitor COOLAIR and COLDAIR of AtFLC, which are derived from the 3' end and the first intron of AtFLC, respectively. Cotton is a worldwide important commercial crop and possesses a variety of different traits. The early maturing cotton has the characteristics of short growth period and rapid growth and development, and can avoid the influence on the quality of cotton in high latitude areas with poor photo-thermal conditions and large air temperature change (such as cotton producing areas like Liaoning, gansu, xinjiang and the like). Therefore, the identification of the cotton flowering related genes, the elucidation of the regulation mechanism and the creation of excellent germplasm resources of the early maturing cotton are of great significance for the healthy and stable development of the cotton industry. Disclosure of Invention The GhWRKY46 gene is cloned from upland cotton, and the over-expression transgenic strain obtained by constructing an over-expression vector and heterologously expressing in Arabidopsis is advanced compared with wild flowering, so that the GhWRKY46 gene plays an important role in controlling cotton flowering period. Provides important application value for cotton variety improvement and breeding. In order to achieve the above purpose, the present invention adopts the following technical scheme: The invention provides application of a gene GhWRKY46 in regulating plant growth and development. The invention also provides application of the over-expression gene GhWRKY46 in promoting plant growth and development. The invention also provides application of the over-expression gene GhWRKY46 in promoting early maturity of plants. The invention also provides application of the over-expression gene GhWRKY46 in promoting the expression of GhFT of plants. The invention also provides application of the over-expression gene GhWRKY46 in advancing the flowering period of plants. In the present invention, the plants and/or plants include cotton. Drawings FIG. 1 shows the quantitative results of GhWRKY 46; FIG. 2 is a graph showing the results of a dual luciferase reporter assay; FIG. 3 shows that GhWRKY46 promotes early flowering of overexpressed transgenic Arabidopsis. Detailed Description The application is further illustrated by the following examples. Before describing the specific embodiments, the following description will briefly explain some experimental contexts in the following embodiments. Examples 1. Cotton material The cotton materials selected by the application are cotton institute 36 in early maturing varieties of upland cotton and cotton 11 in late maturing varieties, wherein the cotton institute 36 and the cotton 11 have extremely obvious differences in flowering time and growth period, and are planted in cotton research institute test fields of China agricultural academy of China under the white wall town of Anyang city in Henan province, and the management measure is normal field management. The sampling mode is that two cotton varieties grow to the young buds of one-leaf stage to five-leaf stag