CN-118873655-B - Material with high photo-thermal conversion efficiency, preparation method thereof and application thereof in nerve regulation and control

Abstract

The invention discloses a material with high photo-thermal conversion efficiency, a preparation method thereof and application thereof in nerve regulation and control, and belongs to the technical field of biomedicine. In the method, liquid metal is ultrasonically treated to nano-scale particles, the liquid metal nano-particles are used as templates, and the hollow nano-shell consisting of the ultra-small metal nano-particles can be prepared through galvanic replacement reaction with metal salts. The material with high photo-thermal conversion efficiency is in a hollow structure, the surface is rough and holes exist, and the special structure endows the material with super photon capturing capability, so that the light absorption is greatly enhanced, and the material has the characteristic of super photo-thermal conversion efficiency. The material is injected into the nerve, and the excellent photo-thermal effect is utilized to rapidly and accurately regulate and control the nerve, so that the effective prevention and control of the autonomic nerve related diseases are realized, and the material has a good application prospect.

Inventors

• FU LEI
• YU LILEI
• ZENG MENGQI
• ZHOU LIPING
• WANG CHENLU
• LIU CHENGZHE
• LIU YAQIAN
• WANG CHENYANG

Assignees

• 武汉大学

Dates

Publication Date

20260505

Application Date

20240725

Claims (7)

1. 1. The preparation method of the material with high light-heat conversion efficiency is characterized by comprising the following steps: (1) The liquid metal belongs to a solvent, and the particle size of liquid metal particles is reduced to the nanometer level through ultrasonic treatment, wherein the liquid metal is gallium; (2) And adding a metal salt solution into the liquid metal nanoparticle dispersion liquid to react, wherein the type of metal salt in the metal salt solution is sodium hexachloroplatinate (IV), and obtaining the hollow metal nanoshell, namely the material with Gao Guangre conversion efficiency, through metal salt galvanic couple substitution reaction.
2. 2. The method for preparing Gao Guangre% conversion efficient material according to claim 1, wherein in the step (1), the time of ultrasonic treatment is 0.5-6 h, the size of gallium nanoparticles in the liquid metal nanoparticle dispersion is 20-500 nm, and the collecting method is that the upper liquid is collected after centrifugation, and the centrifugal force of centrifugation is 10-3000 g, and the time is 0.5-10 min.
3. 3. The method for preparing Gao Guangre% conversion efficiency material according to claim 1, wherein in the step (2), the reaction is performed at normal temperature for a reaction time of 1-4 h, the amount of liquid metal used in the steps (1) and (2) is 0.05-0.5. 0.5 g, the content of metal element in the metal salt solution is 0.05-0.5 mmol, and the concentration of the metal salt solution is 0.01-0.5 mol.L -1 .
4. 4. A material with high light-heat conversion efficiency is characterized in that the material is prepared by adopting the method as set forth in any one of claims 1-3, and the light-heat conversion efficiency of the material with high light-heat conversion efficiency is more than or equal to 70%.
5. 5. Use of the Gao Guangre conversion efficiency material of claim 4 in the manufacture of a medicament for the treatment of ventricular arrhythmias, comprising the steps of: 1) Microinjection of the injection into ganglion, inducing Gao Guangre conversion efficiency material to raise temperature by laser irradiation, and regulating and controlling nerve; 2) Electrically stimulating the laser irradiated ganglion to evaluate the function and activity of the nerve; 3) Based on the results of the functional and activity evaluation of the nerve, the control effect of the disease is evaluated.
6. 6. The use of Gao Guangre conversion efficiency material according to claim 5 in the preparation of a medicament for treating ventricular arrhythmias, wherein in step 1), the concentration of high photothermal conversion efficiency material in injection is 5-400 μg mL -1 , the wavelength range of laser irradiation is 700-1700 nm, the power density is 0.4-1W cm -2 , and the irradiation time is 1-10 min.
7. 7. The use of Gao Guangre-conversion-efficiency materials according to claim 5 for the preparation of a medicament for treating ventricular arrhythmias, wherein in step 2), the frequency of the electrical stimulation is 2-160 Hz, the pulse width is 0.01-0.2 ms, the voltage is set to 5 levels, 0-2V, 2-4V, 4-6V, 6-8V, 8-10V, respectively.

Description

Material with high photo-thermal conversion efficiency, preparation method thereof and application thereof in nerve regulation and control Technical Field The invention relates to the technical field of biomedicine, in particular to a material with high photo-thermal conversion efficiency, a preparation method thereof and application thereof in nerve regulation and control. Background Cardiovascular disease has become a leading cause of death, with acute myocardial infarction being one of the most detrimental diseases. Myocardial ischemia often induces acute ventricular arrhythmias that prevent the patient from being effectively treated in a timely manner. Furthermore, traditional myocardial infarction intervention procedures fail to circumvent the concomitant myocardial reperfusion injury and associated ventricular arrhythmias. Autonomic nervous systems, including sympathetic and parasympathetic nerves, play a role in cardiovascular regulation, both of which are naturally antagonistic. Inhibition of sympathetic nerves or activation of parasympathetic nerves has been shown to stabilize cardiac electrophysiology, prevent myocardial infarction, and reduce the incidence of ventricular arrhythmias. However, cardiac sympathetic denervation, stellate ganglion blocking and renal denervation can cause several adverse effects including Huo Nazeng syndrome, unexpected bleeding and undesirable ablative results. Both conventional vagal nerve stimulation and optogenetic neuromodulation require implantation of an in vivo electrical stimulation or light source device. Furthermore, optogenetic neuromodulation requires viral transfection of photosensitive proteins, and all of the above drawbacks limit the clinical development of these treatments. In recent years, several studies have shown that photo-activated nano-transducers can induce local heating effects, resulting in activation or inhibition of nerves. This finding is due to temperature sensitive ion channels in neurons, such as the transient receptor potential vanilloid 1 (TRANSIENT RECEPTOR POTENTIAL VANILLOID, trpv 1) protein and the TWIK-associated K + channel 1 (TWIK-related K + channel 1, trek 1) protein. The activation of a specific temperature sensitive ion channel requires a precise temperature range. The neural response is acute and therefore there is a need for a therapeutic strategy with rapid and accurate modulation. However, this is still limited by low light-to-heat conversion efficiency. For example, the Chinese patent publication No. CN114767852A discloses an application of a light-controlled release hollow gold-silver nano probe in preparing an integrated preparation for tumor diagnosis and treatment, which is prepared by sequentially adding a reducing agent, a Raman signal molecule and a stabilizing agent into chloroauric acid serving as a gold source and silver nitrate serving as a silver source and adopting a seed growth method. The method is simple and mild, green and environment-friendly, the prepared hollow gold and silver nano particles have multimode imaging functions such as surface enhanced Raman scattering (surface enhancement of RAMAN SCATTERING, SERS) imaging, photo-acoustic (PAT) imaging, fluorescence (FL) imaging and the like, and can realize the accurate visualization of tumor surgical excision; in addition, the hollow nano particles prepared by the method are single in species and narrow in application range, and the application of the hollow nano particles as a photo-thermal conversion material is further limited. In summary, the preparation method of the material with high photo-thermal conversion efficiency, which is simple and easy to operate and wide in application range, meets the treatment strategy requirement of the rapid and accurate regulation of nerve regulation, and has important significance in assessing the functions and activities of nerves and even preventing and treating diseases. Disclosure of Invention In view of the above-mentioned drawbacks of the prior art, in a first aspect of the present invention, a preparation method of a material with high light-heat conversion efficiency, which has a simple process and a wide application range, is provided, comprising the following steps: (1) The liquid metal belongs to a solvent, the particle size of liquid metal particles is reduced to the nanometer level through ultrasonic treatment, and liquid metal nanoparticle dispersion liquid is obtained through collection; (2) And adding a metal salt solution into the liquid metal nanoparticle dispersion liquid, and reacting to obtain the hollow metal nano shell (MNP-shell), namely the material with Gao Guangre conversion efficiency through metal salt galvanic couple substitution reaction. Preferably, in the step (1), the type of the liquid metal includes gallium, indium, tin, bismuth, zinc and alloys thereof; Preferably, in the step (1), the time of the ultrasonic treatment is 0.5-6 h. Preferably, in the step (1), the size of the