CN-118929858-B - Method for inactivating protogenic amoeba and endophytes thereof by electrocatalytic water oxidation

Abstract

The invention belongs to the technical field of drinking water disinfection, and particularly relates to a method for inactivating protozoon amoeba and endophytes thereof by electrocatalytic water oxidation. In order to develop a feasible technology for effectively inactivating amoeba and intracellular microorganisms thereof, the invention provides an electrocatalytic water oxidation method for inactivating amoeba and intracellular organisms thereof based on a high-grade oxidation technology, and the method can generate strong oxidizing substances such as hydrogen peroxide, hydroxyl radicals and the like in situ so as to inactivate amoeba and intracellular bacteria thereof, and has excellent inactivation effect. Meanwhile, the method has the advantages of simple equipment, convenient operation, low cost, environmental protection and the like, and provides a feasible electrocatalytic water oxidation technology for effectively inactivating amoeba and microorganisms in spores thereof.

Inventors

• SHU LONGFEI
• HU ZHUOFENG
• OU ZHESHUN
• WANG ZIHE
• SUN MENGDI
• DUAN CHENGYU

Assignees

• 中山大学

Dates

Publication Date

20260512

Application Date

20240905

Claims (8)

1. 1. A method for inactivating protozoon amoeba and endophytes thereof by electrocatalytic water oxidation is characterized in that a carbon-based material loaded with a water oxidation material is used as an anode, and the amoeba and intracellular microorganisms thereof are inactivated by electrocatalytic water oxidation: firstly, loading a water oxidation material on a carbon-based material to prepare an anode electrode slice, loading nano zero-valent iron on the carbon-based material to prepare a cathode electrode slice, then inserting the anode electrode slice into water containing amoeba, adding bicarbonate electrolyte, and electrifying, so as to inactivate the amoeba and intracellular microorganisms thereof by generating strong oxidizing substances; the water oxidation material comprises bismuth antimonate, antimony tungstate and bismuth tungstate, and is prepared by a hydrothermal method.
2. 2. A method for inactivating protozoan amoeba and their endophytes by electrocatalytic water oxidation according to claim 1, wherein the energizing time is more than 2 hours.
3. 3. A method for inactivating protozoan amoeba and its endophytes by electrocatalytic water oxidation according to claim 1, wherein the concentration of bicarbonate electrolyte is 0.5M-3M.
4. 4. A method for inactivating protozoan amoeba and their endophytes by electrocatalytic water oxidation according to claim 1, wherein the energizing voltage is controlled between 2.7 and 4V relative to the reversible hydrogen electrode.
5. 5. The method for inactivating protozoon amoeba and its endophytes by electrocatalytic water oxidation according to claim 1, wherein the stirring treatment is carried out during the energizing process, and the stirring speed is 100-1000 rpm.
6. 6. The method for inactivating protozoon and endophyte thereof by electrocatalytic water oxidation according to claim 1, wherein the operation of preparing bismuth antimonate by a hydrothermal method comprises the steps of dissolving Sb 2 O 3 and Bi (NO 3 ) 3 ·5H 2 O in water, adjusting pH, placing the mixture in a hydrothermal reaction at 170-190 ℃ for 40-50 h, and finally collecting, washing and drying the precipitate.
7. 7. The method for inactivating protozoon and endophyte thereof by electrocatalytic water oxidation according to claim 1, wherein the operation of preparing antimony tungstate by a hydrothermal method is that SbCl 3 and Na 2 WO 4 ·2H 2 O are dissolved in ethylene glycol, then the ethylene glycol is subjected to hydrothermal reaction at 150-170 ℃ for 7-13 h, and finally the precipitate is collected, washed and dried to obtain the antimony tungstate.
8. 8. The method for inactivating protozoon and endophyte thereof by electrocatalytic water oxidation according to claim 1, wherein the operation of preparing bismuth tungstate by a hydrothermal method is that Bi (NO 3 ) 3 ·5H 2 O and Na 2 WO 4 ·2H 2 O) are dissolved in water, then the bismuth tungstate is subjected to hydrothermal reaction for 20-30 hours at 170-190 ℃, and finally the precipitate is collected, washed and dried to obtain the bismuth tungstate.

Description

Method for inactivating protogenic amoeba and endophytes thereof by electrocatalytic water oxidation Technical Field The invention belongs to the technical field of drinking water disinfection, and particularly relates to a method for inactivating protozoon amoeba and endophytes thereof by electrocatalytic water oxidation. Background At present, the disinfection and sterilization means for the water body mainly comprise chemical means and physical means, wherein the physical means comprise a heating (high temperature) method, a gamma radiation method, an ultraviolet radiation method, a filtering method and the like, and the chemical means comprise disinfection methods of adding heavy metal ions (such as silver and copper), adding alkali or acid, adding a surfactant chemical agent, adding an oxidant and the like. Among the physical means, the most commonly used physical sterilization means for water is a filtration method at present, but due to reasons that a filter element needs to be cleaned and replaced regularly, microorganisms cannot be completely eliminated, and the microorganisms can form a biological film which is difficult to remove in the filter element or a pipeline, and can be enriched in the filter element, so that more serious biosafety risks are caused. Ultraviolet and high-temperature sterilization and other means are generally difficult to be commonly used due to equipment and equipment problems, high cost and other reasons. Among the chemical means, disinfection with oxidants is most widely used, with chlorine and its compounds being the most common for disinfection, followed by ozone disinfection. However, methods of adding heavy metal ions (such as silver and copper), adding alkali or acid, adding surfactant and the like are difficult to apply in many situations due to the fact that the method is not friendly to the environment, byproducts are difficult to remove and the like, and methods of adding oxidizing agents (such as chlorine disinfection and the like) have good inactivation effects on microorganisms such as bacteria and the like, but have unsatisfactory inactivation effects on protozoa (such as amoeba including pathogenic amoeba including amoeba histolytica, acanthamoeba, foggy-resistant amoeba and the like) and endophytes thereof. Therefore, at present, few effective disinfection methods are available for amoeba and endophytes thereof, and common disinfection methods (such as chloridization, chlorine dioxide, ultraviolet rays and the like) cannot effectively inactivate the amoeba. Such as acanthamoeba encapsulation, can tolerate 100mg/L chlorine for 10 minutes, 50mg/L chlorine for 18 hours. Meanwhile, the amoeba spores have good tolerance to strong acid and strong alkali, high salt concentration and high temperature. Even in high concentration hydrogen peroxide, part of amoeba spores can still keep activity. Moreover, the hydrogen peroxide with higher concentration has no good inactivation effect on amoeba encapsulation, and the hydrogen peroxide is used as a common oxidation disinfectant, has high risks of storage, transportation and the like, is easy to decompose, and is still greatly restricted in application on the premise that no good means for preparing the hydrogen peroxide in situ exist at present. In addition, the current main electrochemical disinfection means is an oxygen reduction method, and the inactivation effect on microorganisms such as bacteria is good. However, the traditional oxygen reduction method needs to be carried out under the condition of sufficient oxygen, and is difficult to function under the anoxic condition, and meanwhile, only hydrogen peroxide can be generated, or amoeba cannot be effectively inactivated. Thus, there is a need to explore a viable technique for effectively inactivating amoeba and its intracellular microorganisms. Research shows that the strong oxidative free radicals such as hydroxyl free radicals generated by the advanced oxidation method can effectively inactivate the amoeba protozoa. Therefore, the construction of the water environment electrocatalytic disinfection system by the external power supply device is a disinfection method with application potential. The existing electrocatalytic method mainly comprises two types of electrocatalytic oxygen reduction and electrocatalytic water oxidation, wherein the electrocatalytic oxygen reduction is very dependent on an oxygen exposing device, meanwhile, only hydrogen peroxide can be generated, amoeba protozoa cannot be effectively inactivated, and an activating agent or a device for activating the hydrogen peroxide must be additionally added for generating the activated hydrogen peroxide, so that a plurality of problems are brought to practical application. The electrocatalytic water oxidation does not need an additional device, hydrogen peroxide generated in real time can be activated through the coupling of the reduction reaction of the cathode, meanwhile, because the electrolyte