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CN-118931763-B - Lysobacter P18 and application thereof

CN118931763BCN 118931763 BCN118931763 BCN 118931763BCN-118931763-B

Abstract

The invention belongs to the field of microorganisms, and relates to a lysobacter P18 and application thereof, wherein the lysobacter P18 is lysobacter Lysobacter soli, and the lysobacter P18 is submitted to CGMCC for preservation in the 6 th month 7 of 2024, and the preservation number is NO.30877. The lysobacter P18 provided by the invention has a growth promoting effect on tomatoes.

Inventors

  • LAI HANGXIAN
  • FU WENJIANG
  • SUN CHENYU
  • SUN BIN
  • LI PENGFEI
  • WANG SHIYU
  • GUO QIAO

Assignees

  • 西北农林科技大学

Dates

Publication Date
20260505
Application Date
20240722

Claims (7)

  1. 1. The lysobacter P18 is characterized in that the lysobacter P18 is lysobacter Lysobacter soli, and the lysobacter P18 is submitted to CGMCC for preservation at the date of 7 and 6 of 2024, and the preservation number is NO.30877.
  2. 2. Use of the lysobacter sp 18 of claim 1 for the promotion of tomato growth or for the improvement of tomato fruit quality.
  3. 3. The method according to claim 2, wherein the lysobacter P18 is a cell-free fermentation broth of the lysobacter P18 or a bacterial suspension of the lysobacter P18.
  4. 4. The method according to claim 3, wherein when the lysobacter P18 is a cell-free fermentation broth of the lysobacter P18, the cell-free fermentation broth of the lysobacter P18 is prepared by placing single colonies of the lysobacter P18 cultured in the NA medium at 24h in the NB medium, culturing at 36℃and 160 r/min for 24h, preparing a bacterial suspension having an effective viable count of not less than 1X 10 8 cfu•mL -1 from the bacterial suspension in the NB medium, centrifuging the culture broth 90 mL in a 100mL centrifuge tube at 4℃and 12000 r/min for 10 min, filtering the supernatant with a sterile filter membrane of 0.22. Mu.m, and diluting the filtrate with sterile water to obtain cell-free fermentation broths of different multiples.
  5. 5. The method according to claim 4, wherein when the lysobacter P18 is a bacterial suspension of the lysobacter P18, the bacterial suspension of the lysobacter P18 is prepared by placing single colonies of the lysobacter P18 cultured in the NA medium at 24 h in the NB medium, culturing the single colonies at 36℃and 160 r/min at 24 h, taking the culture solution 90 mL in a 100 mL centrifuge tube, centrifuging the culture solution at 4℃and 12000r/min at 10 min, discarding the supernatant, and preparing a bacterial suspension at 1X 10 8 cfu·mL -1 with 1X PBS buffer.
  6. 6. The application according to claim 3, wherein the application is executed by a root application.
  7. 7. The method of claim 6, wherein the root application is performed at a distance of 1.5cm-2cm from the periphery of the root of the tomato.

Description

Lysobacter P18 and application thereof Technical Field The invention belongs to the field of microorganisms, relates to a lysobacter and application thereof, and particularly relates to a lysobacter P18 and application thereof. Background Tomatoes are one of the most important horticultural crops in the world and are also an important source of nutrition for humans. Tomatoes have become one of the most popular vegetables and fruits in the world because of their unique flavor and high nutritional value, and are also an important source of trace elements in the human diet. Indeed, tomatoes are the second most commonly consumed fruits and vegetables in many western countries. The global tomato consumption has increased by approximately three times over the past 40 years. Therefore, the global economic importance of tomatoes and their nutritional value is undoubted. Lysobacte (Lysobacter) is a class of gram-negative bacilli with slip properties, high g+c content, safe to humans and animals, and lysozyme active against a variety of pathogenic fungi, bacteria and nematodes. The genus lysobacter is now belonging to the phylum gamma-proteobacteria, order xanthomonas, family xanthomonas. Its distribution is very wide, and it exists in soil (rhizosphere), river, sewer and other environments. The early stage divided the genus lysobacter into 4 species based on the strain phenotype, lysobacter enzymogenes (Lysobacterenzymogenes), lysobacter antibioticus (Lysobacterantibioticus), lysobacter palmi (Lysobacterbrunescens) and lysobacter gelatinosum (Lysobactergummosus), respectively. The lysobacter reported in the literature is mainly lysobacter enzymogenes, has remarkable antibacterial effects on sclerotinia rot of colza, soft rot of sweet potato, phytophthora capsici and Pythium aphanidermatum and the like (Qian G L et al, 2010, biological Control), and can effectively prevent and treat festuca arundinacea leaf spot caused by Bipolarissorokiniana (Kilic-Ekici O et al, 2003, phytopathology) and turf brown spot caused by Rhizoctonia solani (Yuan G Y,2001, international Turfgrass Society Research Journal). Ji Anhai et al reported that the antibiotic lysobacter 13-1 can inhibit various pathogenic bacteria such as bacterial leaf blight of rice (J i G H et al, 2008, biological Contro). The related patents of the genus Lysobacter are mainly the enzyme-producing lysobacter (patent application numbers: 200710190998.6 and 201310207022.0) and the antibiotic-producing lysobacter (patent application numbers: 200510011078.4 and 201310405482.4). Lysobacter is one of the common plant rhizosphere-promoting bacteria, most members of this genus having antagonistic activity against a range of other microorganisms, including gram-negative and gram-positive bacteria, fungi, oomycetes and nematodes (Reichenbach 2006). They are therefore known to produce a variety of extracellular enzymes and antimicrobial compounds (VASILYEVA ET al 2014). Although there has been some previous study on lysobacter, most of the focus has been on its disease resistance and disease resistance mechanism, and studies on the growth of plants (e.g., tomatoes) and fruit quality of lysobacter have not been reported yet. Disclosure of Invention In order to solve the technical problems in the background art, the invention provides a lysobacter P18 with a growth promoting effect on tomatoes and application thereof. In order to achieve the above purpose, the present invention adopts the following technical scheme: the lysobacter P18 is characterized in that the lysobacter P18 is lysobacter Lysobacter soli, and the lysobacter P18 is submitted to CGMCC for preservation at the date of 7 and 6 of 2024, and the preservation number is NO.30877. The use of the aforementioned lysobacter P18 for promoting the growth of crops or improving the quality of fruits. Use of the aforementioned lysobacter P18 for promoting tomato growth or improving fruit quality. The use as described above, characterized in that the lysobacter P18 is a cell-free fermentation broth of the lysobacter P18 or a bacterial suspension of the lysobacter P18. When the lysobacter P18 is a cell-free fermentation broth of the lysobacter P18, the cell-free fermentation broth of the lysobacter P18 is prepared by placing single bacterial colonies of the lysobacter P18 cultured in an NA medium at 24h in an NB medium, culturing 24h at 36 ℃ and 160 r/min, preparing a bacterial suspension with an effective viable count of not less than 1X 10 8cfu•mL-1 by using the NB medium, centrifuging the culture broth 90 mL in a100 mL centrifuge tube at 4 ℃ and 12000 r/min for 10min, filtering the supernatant with a sterile filter membrane of 0.22 mu m, and diluting the filtrate with sterile water to obtain cell-free fermentation broths with different multiples. When the lysobacter P18 is a bacterial suspension of the lysobacter P18, the bacterial suspension of the lysobacter P18 is prepared by placing a single colony of the lysobacter P18