CN-118956951-B - Application of RcETC gene in regulating plant flower color

Abstract

The invention provides an application of RcETC gene in regulating plant flower color. The first aspect of the invention provides an application of RcETC gene in regulating plant flower color, wherein RcETC gene is one of A1) -A3), A1) a nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1, A2) a nucleotide sequence derived from the nucleotide sequence shown as SEQ ID NO. 1 through substitution, deletion or addition of one or more nucleotides, and A3) a nucleotide sequence with at least 80% identity with SEQ ID NO. 1. According to the invention, the transcription factor RcETC3 is cloned from the China rose 'month powder', and experiments show that the color of the China rose petals is obviously deepened in the transient gene silencing strain of the China rose, which shows that the RcETC gene plays an important role in regulating and controlling the flower color of the China rose, and provides an important basis for China rose breeding.

Inventors

• HE GUOREN
• WANG BINGSHUANG
• MING FENG
• MENG QI

Assignees

• 上海师范大学

Dates

Publication Date

20260512

Application Date

20240929

Claims (4)

1. 1. The application of the silenced RcETC gene in deepening the flower color of the month powder China rose is characterized in that the RcETC gene is a nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1.
2. 2. The use according to claim 1, characterized in that silencing the expression of RcETC gene in the lunar roses comprises in particular: Operably linking the open reading frame of the RcETC gene to a pTRV2 vector to form a TRV2-RcETC3 recombinant vector containing the RcETC gene; Introducing the TRV2-RcETC3 recombinant vector into agrobacterium, and obtaining the RcETC gene-silenced transgenic YueyueXtensible rose by an agrobacterium-mediated method.
3. 3. A method for deepening the flower color of the month powder China rose is characterized by silencing the expression of RcETC genes in the month powder China rose, wherein the RcETC genes are nucleic acid molecules with nucleotide sequences shown as SEQ ID NO. 1.
4. 4. The method according to claim 3, wherein silencing the expression of RcETC gene in the lunar roses comprises: Operably linking the open reading frame of the RcETC gene to a pTRV2 vector to form a TRV2-RcETC3 recombinant vector containing the RcETC gene; Introducing the TRV2-RcETC3 recombinant vector into agrobacterium, and obtaining the RcETC gene-silenced transgenic YueyueXtensible rose by an agrobacterium-mediated method.

Description

Application of RcETC gene in regulating plant flower color Technical Field The invention relates to the technical field of genetic engineering, in particular to application of RcETC genes in regulating and controlling plant flower colors. Background China rose (Rosa chinensis Jacq.) belongs to Rosa genus of Rosaceae family, and perennial woody plants are bright in color, and development of rich and diverse colors is one of main targets of flower breeders. The flower color of China rose is mainly determined by anthocyanin, in anthocyanin synthesis, the expression of enzyme genes is mainly controlled by transcription factors, and 3 types of transcription factors related to anthocyanin synthesis control, namely MYB type, bHLH and WD40, are found, and generally form MBW complex to promote or inhibit anthocyanin biosynthesis. MYB genes are widely distributed in higher plants and are one of the largest families of transcription factors. Currently, a great deal of research indicates that MYB factors can regulate anthocyanin synthesis, wherein R2R3-MYB and R3-MYB transcription factors have been identified as inhibitors of anthocyanin synthesis, and can influence plant flower color by reducing anthocyanin biosynthesis and accumulation. The regulation of these transcription factors is embodied in different plant species, for example PsMYB in peony is used as transcription inhibitor, and its expression is inhibited by specific binding to PsDFR gene promoter, so that the area of petal stain and total anthocyanin content are reduced, and StMYBATV protein in potato can be combined with bHLHs protein to prevent anthocyanin biosynthesis in potato cell culture. How to excavate transcription factors involved in color regulation in China rose is one of main research contents of China rose breeding. Disclosure of Invention The invention provides an application of RcETC gene in regulating plant flower color. The first aspect of the present invention provides an application of RcETC gene in regulating plant flower color, wherein the RcETC gene is one of A1) -A3): a1 A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1; A2 1) a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO. 1 by substitution, deletion, or addition of one or more nucleotides; A3 A nucleotide sequence having at least 80% identity to SEQ ID NO. 1. Further, expression of RcETC gene was down-regulated to darken the plant petals. The method is characterized in that the anthocyanin content in plant petals is improved. Further, down-regulating the expression of RcETC gene includes silencing the expression of RcETC gene in plants. Further, silencing expression of RcETC gene in plants specifically includes: Operably linking the open reading frame of the RcETC gene to a pTRV2 vector to form a TRV2-RcETC3 recombinant vector containing the RcETC gene; Introducing the TRV2-RcETC3 recombinant vector into agrobacterium, and obtaining the RcETC gene-silenced transgenic plant by an agrobacterium-mediated method. Further, the plant is China rose. The second aspect of the invention provides a method for regulating plant flower color, which regulates the expression of RcETC genes in plants, wherein the RcETC genes are one of A1) -A3): a1 A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 1; A2 1) a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO. 1 by substitution, deletion, or addition of one or more nucleotides; A3 A nucleotide sequence having at least 80% identity to SEQ ID NO. 1. Further, modulating expression of the RcETC gene in a plant includes down-regulating expression of the RcETC gene. Further, down-regulating the expression of RcETC gene includes silencing the expression of RcETC gene in plants. Further, silencing expression of RcETC gene in plants specifically includes: Operably linking the open reading frame of the RcETC gene to a pTRV2 vector to form a TRV2-RcETC3 recombinant vector containing the RcETC gene; Introducing the TRV2-RcETC3 recombinant vector into agrobacterium, and obtaining the RcETC gene-silenced transgenic plant by an agrobacterium-mediated method. Further, the plant is China rose. According to the invention, the transcription factor RcETC3 is cloned from the China rose 'month powder', and experiments show that the color of the China rose petals is obviously deepened in the transient gene silencing strain of the China rose, which shows that the RcETC gene plays an important role in regulating and controlling the flower color of the China rose, and provides an important basis for China rose breeding. Drawings FIG. 1 is a photograph of buds or flowers of China rose 'month powder' at different developmental stages; FIG. 2 shows anthocyanin content in buds or flowers of China rose 'month powder' at different development periods, wherein different letters represent difference significance among groups, t test is carried out, and p is less than 0.05; FIG. 3 show