CN-119080236-B - Mixed nutrition type denitrification electron donor and construction method thereof

CN119080236BCN 119080236 BCN119080236 BCN 119080236BCN-119080236-B

Abstract

The application provides a mixed nutrition type denitrification electron donor and a construction method thereof, which relate to the field of biological denitrification and comprise the steps of adopting a simulation reactor to simulate a sewage anoxic tank to culture denitrifying bacteria by taking a liquid sulfur source as the electron donor, and finishing domestication of sulfur autotrophic denitrifying bacteria; the sewage treatment anaerobic tank denitrification system comprises a sewage plant anaerobic tank, a denitrification load and a pH value adjusting device, wherein the sewage plant anaerobic tank is used for controlling the hydraulic retention time to be consistent with that of the anaerobic tank, the denitrification load and the pH value adjusting device are adjusted to a preset range by adding a carbon source or an alkaline buffer, and the agent proportion is determined according to the added liquid sulfur source, carbon source or alkaline buffer and is used for preparing a mixed nutritional denitrification electron donor which is used as the denitrification electron donor of the sewage treatment anaerobic tank denitrification system. The application can obtain the mixed denitrification electron donor aiming at the sewage quality, and reduce the organic carbon source consumption.

Inventors

PENG MENGWEN
CHEN YASONG
HUI ERQING
ZHOU XIAOGUO
LIU MENGMENG
LI LI
JIA BOYANG
SUN WAN

Assignees

中国长江三峡集团有限公司
长江生态环保集团有限公司

Dates

Publication Date: 20260505
Application Date: 20240924

Claims (7)

1. The construction method of the mixed nutrition type denitrification electron donor is characterized by comprising the following steps: Adopting a simulation reactor to simulate a sewage anoxic tank to culture denitrifying bacteria by taking a liquid sulfur source as an electron donor, and finishing domestication of sulfur autotrophic denitrifying bacteria; Controlling the hydraulic retention time to be consistent with that of an anoxic tank of a sewage plant by taking water fed into the anoxic tank as a water source, culturing heterotrophic denitrifying bacteria by adding a carbon source to adjust the denitrification load until the denitrification load is not less than a preset load value, recording the total adding amount of the carbon source, and adjusting the pH value to a preset range by an alkaline buffer, wherein the heterotrophic denitrifying bacteria and the sulfur autotrophic denitrifying bacteria are in the same simulation reactor; the method for culturing denitrifying bacteria by using a simulated reactor to simulate a sewage anoxic tank and using a liquid sulfur source as an electron donor, and finishing domestication of sulfur autotrophic denitrifying bacteria comprises the following steps: inoculating sludge in an anoxic pond of a sewage plant in a simulation reactor, taking inflow water of the anoxic pond of the sewage plant as a water source, and determining the addition amount of the liquid sulfur source according to the concentration of nitrate radical in the simulation reactor, wherein the addition amount of the liquid sulfur source is determined according to the ratio of the amount of substances of sulfur element to the amount of substances of the liquid sulfur source, and the amount of substances of the sulfur element is determined according to the nitrogen-sulfur ratio of 1:1-1:2; And monitoring the nitrate concentration in the simulation reactor, acquiring a denitrification rate according to the nitrate concentration, and determining that the sulfur autotrophic denitrifying bacteria acclimation is successful if the denitrification rate tends to be stable.
2. The method according to claim 1, wherein the culturing of heterotrophic denitrifying bacteria by adding a carbon source to adjust denitrification load, and the adjusting of pH to a preset range by an alkaline buffer, comprises: acquiring denitrification load in the denitrification process according to the nitrate concentration; Judging whether the denitrification load is smaller than the preset load value or not; If the denitrification load is smaller than the preset load value, adding a carbon source to the simulation reactor until the denitrification load is not smaller than the preset load value; And if the pH value is smaller than the preset pH value, adding an alkaline buffer until the pH value is not smaller than the preset pH value.
3. The method according to claim 1, wherein the amount of the substance of elemental sulfur is determined based on a nitrogen to sulfur ratio of 1:1.1.
4. The method of claim 2, wherein said obtaining the denitrification load in the denitrification process as a function of nitrate concentration comprises: acquiring the nitrate concentration before and after a preset period, and acquiring the denitrification rate of the period according to the ratio of the nitrate concentration variation to the corresponding time interval; and obtaining the denitrification load according to the denitrification rate.
5. The method of claim 1, wherein the liquid sulfur source is sodium sulfide or liquid sodium thiosulfate.
6. The method of claim 2, wherein the predetermined pH is set in the range of 6.5 to 8.0.
7. The method of claim 2, wherein the alkaline buffer is sodium carbonate or sodium bicarbonate.

Description

Mixed nutrition type denitrification electron donor and construction method thereof Technical Field The application relates to the field of biological denitrification, in particular to a mixed nutrition type denitrification electron donor and a construction method thereof. Background Denitrification is a microorganism-mediated biochemical process that occurs primarily in anoxic environments, involving the reduction of nitrate (NO 3-) and nitrite (NO 2-) to gaseous nitrogen compounds (such as N 2、N2 O), which ultimately release active nitrogen in the water or soil to the atmosphere in the form of nitrogen. In sewage treatment, the anaerobic or anoxic treatment unit is designed and operated to promote the growth of denitrifying bacteria and denitrification reaction, so that the nitrogen in the sewage is effectively removed, and the emission standard or resource recovery requirement is met. The denitrification process has important value for nitrogen balance of an ecological system, sewage treatment and environmental protection. The traditional heterotrophic denitrification process relies on an organic carbon source, and for sewage plants with insufficient water inlet carbon sources, the additional organic carbon sources such as glucose, sodium acetate and the like are needed, so that the operation cost is high, and the risk of carbon source penetration possibly exists. In view of the above drawbacks, there is a need for a mixed nutritional type denitrification nitrogen removal electron donor and a construction method thereof, which can obtain a mixed denitrification nitrogen removal electron donor for wastewater quality and reduce organic carbon source consumption. Disclosure of Invention The application provides a mixed nutrition type denitrification nitrogen removal electron donor and a construction method thereof, which can obtain the mixed denitrification nitrogen removal electron donor aiming at sewage quality and reduce organic carbon source consumption. In a first aspect, the application provides a method for constructing a mixed nutritional denitrification nitrogen removal electron donor, comprising the following steps: Adopting a simulation reactor to simulate a sewage anoxic tank to culture denitrifying bacteria by taking a liquid sulfur source as an electron donor, and finishing domestication of sulfur autotrophic denitrifying bacteria; Controlling the hydraulic retention time to be consistent with that of an anoxic tank by taking the inflow water of the anoxic tank of a sewage plant as a water source, and adjusting denitrification load and pH value to a preset range by adding a carbon source or an alkaline buffer; And determining the proportion of the medicament according to the added liquid sulfur source, carbon source or alkaline buffer, wherein the proportion is used for preparing a mixed nutritional type denitrification electron donor which is used as a denitrification electron donor of the sewage treatment anoxic tank denitrification system. In one possible design, the method for adjusting denitrification load and pH to a preset range by adding a carbon source or an alkaline buffer comprises the following steps: acquiring denitrification load in the denitrification process according to the nitrate concentration; Judging whether the denitrification load is smaller than the preset load value or not; If the denitrification load is smaller than the preset load value, adding a carbon source to the simulation reactor until the denitrification load is not smaller than the preset load value; And if the pH value is smaller than the preset pH value, adding an alkaline buffer until the pH value is not smaller than the preset pH value. In one possible design, the training of denitrifying bacteria by using a simulated sewage anoxic tank with a liquid sulfur source as an electron donor to complete the domestication of sulfur autotrophic denitrifying bacteria comprises the following steps: inoculating sludge in an anoxic tank of a sewage plant in a simulation reactor, and taking inflow water of the anoxic tank of the sewage plant as a water source; And monitoring the nitrate concentration in the simulation reactor, acquiring a denitrification rate according to the nitrate concentration, and determining that the sulfur autotrophic denitrifying bacteria acclimation is successful if the denitrification rate tends to be stable. In one possible design, the determining the amount of the liquid sulfur source added based on the concentration of nitrate includes: Determining the amount of the substance of the required sulfur element according to the nitrogen-sulfur ratio of 1:1.1; The addition amount of the liquid sulfur source is determined according to the ratio of the amount of the substance of the sulfur element and the amount of the substance of the sulfur element in the liquid sulfur source. In one possible design, the obtaining the denitrification load in the denitrification process according to the nitrate c