CN-119120496-B - ZmRLR1 gene and application of coded protein thereof in regulation and control of corn root system development

Abstract

The invention discloses ZmRLR gene and application of coding protein thereof in regulating and controlling corn root system development. The nucleotide sequence of ZmRLR gene is shown in sequence table SEQ ID No. 1, and the protein sequence is shown in sequence table SEQ ID No. 2. The number of root stocks, the total length of the lateral roots and the total lateral root density of the root system of the ZmRLR protein over-expression transgenic plant in the seedling stage are all obviously increased, the number of root systems and the size of the root system of the fifth crown root in the mature stage are obviously increased, and the weight of rhizosphere soil is increased.

Inventors

• LI WENXUE
• DU QINGGUO
• LV WENSHUAI

Assignees

• 中国农业科学院作物科学研究所

Dates

Publication Date

20260508

Application Date

20240909

Claims (3)

1. The application of ZmRLR1 gene or ZmRLR protein in up-regulating corn root system development is characterized in that the nucleotide sequence of ZmRLR gene is shown as SEQ ID No. 1, and the amino acid sequence of ZmRLR protein is shown as SEQ ID No. 2.
2. 2. The use of claim 1, wherein the ZmRLR gene or ZmRLR protein increases the root system of corn plants, increases the total length of root system of fifth crown root, and increases the weight of rhizosphere soil.
3. 3. A method for increasing the root system of corn is characterized in that ZmRLR gene is over-expressed in corn plant body, and the nucleotide sequence of ZmRLR gene is shown as SEQ ID No. 1.

Description

ZmRLR1 gene and application of coded protein thereof in regulation and control of corn root system development Technical Field The invention belongs to the technical field of biology, and particularly relates to ZmRLR gene and application of coding protein thereof in regulation and control of corn root system development. Background Increasing corn yield is a significant need in the current corn industry. Increasing corn planting density is an important measure to increase corn yield. However, an increase in corn planting density increases the risk of corn lodging. The root system is used as an important organ for absorbing moisture and nutrient of plants, and good root system configuration is beneficial to enhancing lodging resistance of crops and efficient absorption and utilization efficiency of nutrient. In particular, lateral roots act as important organs for plants to absorb moisture, nutrients and to cope with stress, and play a vital role in adapting to various environmental conditions. Therefore, the development of key genes for regulating and controlling the development of corn root systems is of great significance for cultivating new lodging-resistant corn varieties. Disclosure of Invention The invention aims to provide ZmRLR gene and application of coded protein thereof in regulating and controlling corn root system development. ZmRLR1 gene, wherein the polynucleotide of the ZmRLR gene is represented by (a), (b), (c) or (d): (a) A polynucleotide shown as a sequence table SEQ ID No.1 or (B) Polynucleotide which can hybridize with the complementary sequence of SEQ ID No. 1 under stringent hybridization condition, the protein encoded by the polynucleotide still has the function of regulating and controlling the development of corn root system; (c) A polynucleotide having at least 90% or more homology to the polynucleotide shown in SEQ ID No. 1, or (D) The polynucleotide mutant obtained by deleting, substituting or inserting one or more bases on the basis of the polynucleotide shown in SEQ ID No.1 still has the function of regulating and controlling the development of corn root systems. ZmRLR1 protein, wherein the amino acid sequence of the ZmRLR protein is shown as (a), (b) or (c): (a) An amino acid sequence shown as a sequence table SEQ ID No. 2 or (B) Amino acid having at least 90% or more homology with the amino acid shown in SEQ ID No.2, or (C) The protein mutant obtained by deleting, substituting or inserting one or more amino acids on the basis of the protein shown in SEQ ID No. 2 still has the function of regulating and controlling the development of corn root systems. A primer for amplifying any fragment of the ZmRLR gene. Recombinant expression vectors containing the ZmRLR gene. The ZmRLR gene or the ZmRLR protein is applied to regulation and control of corn root system development. The ZmRLR gene or ZmRLR1 protein increases the root system of the corn plant, increases the total length of the root system of the fifth crown root and increases the weight of rhizosphere soil. A method for increasing corn root system comprises over-expressing ZmRLR gene in corn plant. The invention has the beneficial effects that the corn ZmRLR gene and the coding protein thereof can regulate and control the development of corn root systems. The number of root stocks, the total length of the lateral roots and the total lateral root density of the root system of the ZmRLR protein over-expression transgenic plant in the seedling stage are all obviously increased, the number of root systems and the size of the root system of the fifth crown root in the mature stage are obviously increased, and the weight of rhizosphere soil is increased. Drawings FIG. 1 shows the relative expression of ZmRLR1 at each tissue site in maize seedling and mature stages. FIG. 2 shows the root phenotype of rlr 1.1 ems mutants at seedling and maturity; In the figure, a is a mutation site schematic diagram of a rlr1 ems mutant, b is the number of lateral root primordia of main roots after the rlr1 ems mutant is subjected to water culture for 7 days, c is the lateral root density of root systems after the rlr1 ems mutant is subjected to water culture for 7 days, d is the lateral root total length of root systems after the rlr1 ems mutant is subjected to water culture for 7 days, e is the root system phenotype of the rlr1 ems mutant in the mature period, f is the total length of fifth-layer crown roots of the rlr1 ems mutant in the mature period, and g is the rhizosphere soil weight of the rlr1 ems mutant in the mature period. FIG. 3zmrlr construction and identification of knockout mutants; In the figure, a is a target site of zmrlr1 knockout mutant, and b is an editing type of zmrlr1 knockout mutant. FIG. 4 is ZmRLR identification of transgenic plants overexpressing ZmRLR. FIG. 5 is a root phenotype analysis of ZmRLR knockout mutants and overexpressing transgenes; in the graph, a is the number of the lateral root primordia of the main root