CN-119157697-B - Preparation method of pH response cotton swab for wound infection monitoring

Abstract

A method for preparing pH response cotton swab for monitoring wound infection includes such steps as immersing natural cotton fibres in bromocresol green solution for dyeing, flushing the immersed natural cotton fibres with buffer solution, squeezing out excess buffer solution, baking to obtain orange-yellow natural cotton fibres, and fixing the dried natural cotton fibres to the top of slender rod. The invention uses the color change of the pH response cotton swab after the wound is wiped, rapidly judges the type of wound infection of a patient, wherein the pH value is 4.0-6.0 corresponding to an acute wound, the pH value is 7.0-10.0 corresponding to a chronic wound, and adopts a corresponding treatment strategy based on the wound type, thereby being beneficial to improving the working efficiency of medical staff.

Inventors

• JI HUA
• HU YUFAN
• XIAO SHILI
• XIA LIANGJUN
• LIU YUHANG
• XU WEILIN
• LIU LEI

Assignees

• 稳健医疗(武汉)有限公司
• 武汉纺织大学

Dates

Publication Date

20260508

Application Date

20240805

Claims (7)

1. 1. A method for preparing a pH response cotton swab for wound infection monitoring, which is characterized by comprising the following steps: the preparation method comprises the following steps: soaking natural cotton fibers in a bromocresol green solution for dyeing, wherein the color of the bromocresol green solution is blue in the dyeing process; Washing the soaked natural cotton fiber with a buffer solution, wherein the buffer solution is phosphate buffer solution or acetic acid buffer solution, and the pH value of the buffer solution is 1.0-3.0; extruding redundant buffer solution, drying the natural cotton fiber, and making the dried natural cotton fiber orange-yellow; And fixing the dried natural cotton fibers at the top end of the slender rod to obtain the pH response cotton swab.
2. 2. The method of claim 1, wherein the staining is performed at 60-90 ℃ for 20-30min.
3. 3. The method for preparing a pH responsive cotton swab for wound infection monitoring according to claim 1 or 2, wherein the bromocresol green solution is obtained by dissolving bromocresol green powder in ethanol water solution.
4. 4. The method of claim 3, wherein the mass ratio of bromocresol green powder to natural cotton fiber is 0.5-5:10.
5. 5. The method of claim 4, wherein the ratio of the natural cotton fiber to the bromocresol green solution is 1 g/50 mL.
6. 6. The method of claim 5, wherein the volume ratio of ethanol to deionized water in the aqueous ethanol solution is 1:1-9:1.
7. 7. A method of preparing a pH-responsive swab for wound infection monitoring according to claim 1 or 2, wherein: the drying temperature is 40-60 ℃.

Description

Preparation method of pH response cotton swab for wound infection monitoring Technical Field The invention belongs to the technical field of medical instruments, and particularly relates to a preparation method of a pH response cotton swab for wound infection monitoring. Background Wound microbial infection is a serious problem that can hinder healing of chronic wounds. The method can accurately, timely and early discover the infection, monitor various wound healing biomarkers related to the infection in real time, and is of great help to the treatment and nursing of chronic wounds. However, clinical methods for periodic assessment and care of wounds require a physical visit to a wound care clinic or hospital and require specialized healthcare personnel to regularly spend time judging wound infection. The rapid detection of infection, especially chronic wound infection, is an important step in improving wound management and reducing antibiotic therapy, and medical staff can rapidly give diagnosis results and take measures to treat infected wounds in time, which is important for wound healing. The pH of wound exudates is a key parameter in determining wound infection. Many physiological processes such as inflammatory response, collagen formation and angiogenesis affect changes in the pH of wound exudates. Thus, monitoring the wound pH helps to determine the infection of the wound. The dressing takes bacterial cellulose gel produced by fermenting acetobacter xylinum as a matrix, and generates fluorescent substances through enzymatic reaction of 4-methylumbelliferyl-beta-D glucuronide loaded with fluorescent substrate and beta-glucuronidase (beta-GUS) secreted by bacteria to quickly and in-situ detect bacterial infection, so that whether the wound is infected can be directly judged by observing fluorescence. Similarly, publication number CN201910621326.9 discloses a method for preparing pH-responsive photonic crystal gel matrix for skin wound treatment, which prepares CS/PAA/nano Cu 2WSe4 composite hydrogel and loads pH-responsive photonic crystal matrix, because the wound infection site is weakly acidic, the pH-responsive photonic crystal matrix can perform color reaction under bragg diffraction, and can judge whether the wound is infected or not by naked eye observation of color change. The problems of complex preparation process, high cost, inconvenient use and the like exist in the above technology, and how to prepare the pH response cotton swab which is low in cost, convenient to use and capable of being used for detecting wound infection conditions is a problem to be solved at present. Disclosure of Invention The invention aims at solving the problems existing in the prior art and provides a preparation method of a pH response cotton swab for wound infection monitoring, which is simple in preparation process and low in cost. In order to achieve the above object, the technical scheme of the present invention is as follows: a method of making a pH-responsive cotton swab for wound infection monitoring, the method comprising: soaking natural cotton fibers in a bromocresol green solution for dyeing, wherein the color of the bromocresol green solution is blue in the dyeing process; washing the soaked natural cotton fiber with buffer solution; Extruding redundant buffer solution and drying the natural cotton fiber; And fixing the dried natural cotton fibers at the top end of the slender rod to obtain the pH response cotton swab. The dyeing is carried out at 60-90 ℃ for 20-30min. The bromocresol green solution is obtained by dissolving bromocresol green powder in ethanol aqueous solution. The mass ratio of the bromocresol green powder to the natural cotton fiber is 0.5-5:100. The mass volume ratio of the natural cotton fiber to the bromocresol green solution is 1g:50mL. The volume ratio of the ethanol to the deionized water in the ethanol water solution is 1:1-9:1. The buffer solution is phosphate buffer solution or acetic acid buffer solution. The pH value of the buffer solution is 1.0-3.0. The pH value of the buffer solution is 4.0-5.0. The drying temperature is 40-60 ℃. Compared with the prior art, the invention has the beneficial effects that: The invention relates to a preparation method of a pH response cotton swab for wound infection monitoring, which comprises the steps of firstly, soaking natural cotton fibers in a bromocresol green solution for dyeing, wherein the color of the bromocresol green solution is blue in the dyeing process; washing the soaked natural cotton fiber with buffer solution, extruding excessive buffer solution, stoving, and fixing the stoved natural cotton fiber to the top of the slender rod to obtain pH responding cotton swab. The method can rapidly judge the pH value of the wound of a patient by utilizing the color change of the prepared pH response cotton swab after the wound is wiped, judge the type of wound infection according to the pH value of the wound, judge the type of the