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CN-119223846-B - Flow cytometer capable of improving particle resolution and application method thereof

CN119223846BCN 119223846 BCN119223846 BCN 119223846BCN-119223846-B

Abstract

The invention belongs to the field of cell detection, and particularly relates to a flow cytometer capable of improving particle resolution and a use method thereof, comprising a protective shell, a first lens, a second lens and an optical filter, wherein a partition plate is fixedly welded in the protective shell, an adjustable flow chamber is arranged in the protective shell, a uniform processing assembly is arranged in the protective shell, a second hydraulic rod is arranged at the top end of the interior of the protective shell, the bottom end of the second hydraulic rod is fixedly connected with a mounting plate, a first through hole is formed in the mounting plate in a penetrating manner, positioning blocks are fixedly connected to the top ends of the first lens, the second lens and the optical filter, a laser is fixedly arranged on the side end face of the partition plate, and a self-cleaning assembly is arranged in the protective shell, so that the problem that the conventional flow cytometer cannot conveniently adjust the diameter of a flow channel according to different types of samples is solved; and the problem of being unable to automatically clean the individual optical filters that need to be used.

Inventors

  • QIAN XUEQING
  • LU FENG
  • Hou Juanjie

Assignees

  • 上海润达榕嘉生物科技有限公司

Dates

Publication Date
20260505
Application Date
20241008

Claims (8)

  1. 1. A flow cytometer capable of improving particle resolution comprises a protective housing (1), a first lens (14), The novel optical filter is characterized in that a partition plate (2) is fixedly welded in the protective housing (1), a first door plate (3) and a second door plate (4) are mounted on the protective housing (1), a first hydraulic rod (5) is mounted at the inner bottom end of the protective housing (1), a placing frame (6) is fixedly connected to the top end of the first hydraulic rod (5), a test tube (7) is arranged in the placing frame (6), an adjustable flow chamber (8) is mounted in the protective housing (1), a uniform treatment assembly (9) is mounted in the protective housing (1), a supporting plate (10) is fixedly welded on the inner wall of the protective housing (1) and the side end face of the partition plate (2), a second hydraulic rod (11) is mounted at the inner top end of the protective housing (1), a mounting plate (12) is fixedly connected to the bottom end of the second hydraulic rod (11), a first through hole (13) is formed in a penetrating mode on the mounting plate (12), and a first lens (14) is arranged in a penetrating mode The top of second lens (15) and light filter (16) all fixedly connected with locating piece (17), install on the side terminal surface of division board (2) and be fixed with laser instrument (22), install on the side terminal surface of protection shell (1) and be fixed with first detector (23) and second detector (24), install in protection shell (1) from cleaning up subassembly (25), the side end of protection shell (1) bottom runs through sliding connection has collecting box (26), fixedly connected with magnetic plate (27) on the side terminal surface of collecting box (26), magnetic plate (27) magnetic adsorption connects on the bottom side terminal surface of protection shell (1), be provided with second sponge frame (28) in collecting box (26), fixedly connected with sponge board (29) in second sponge frame (28), install on the side terminal surface of protection shell (1) and be fixed with control panel (30), adjustable flow chamber (8) are including backup pad (801), backup pad (801) welded fastening is on the side terminal surface of division board (2), backup pad (801) are fixed with on the side terminal surface of division board (802), fixed with screw thread frame (803) are fixed on the bottom side terminal surface of protection shell (1), the fixed with screw frame (803) is fixed on the top (803), the bottom rotation of threaded rod (804) is connected with connecting plate (805), articulated on the bottom terminal surface of connecting plate (805) have push rod (806), the bottom of push rod (806) articulates there is first guide plate (807), welded fastening has flexible loop bar (808) on the side terminal surface of first guide plate (807), the other end welded fastening of flexible loop bar (808) is on the inner wall of fixed frame (803), the bottom fixedly connected with first rubber piece (809) and first rubber plate (812) of first guide frame (802), the bottom fixedly connected with second guide plate (810) of first rubber piece (809), the bottom fixedly connected with second rubber piece (811) of second guide plate (810), the bottom fixedly connected with of second guide plate (811) is on the top of first guide plate (807), the side terminal surface of second guide plate (810) and the side terminal surface of first guide plate (807) are all fixed mutually with the side terminal surface of first guide plate (812), first guide plate (807) and first guide plate (807) are provided with fourth guide plate (807) alternately, fourth guide plate (807) and fourth guide plate (807) are provided with respectively, fourth guide plate (807) are provided with, A second deflector (810), The first guide plates (807) are in one-to-one correspondence with the threaded rods (804) through connecting plates (805) on the push rods (806), the outer diameters of the fixing frames (803) are smaller than the diameters of the first through holes (13), second guide frames (813) are fixedly connected to the inner top end faces of the first guide frames (802), third guide plates (814) and second guide plates (818) are fixedly connected to the bottom ends of the second guide frames (813), third guide plates (815) are fixedly connected to the bottom ends of the third guide plates (814), fourth guide plates (816) are fixedly connected to the bottom ends of the third guide plates (815), fourth guide plates (817) are fixedly connected to the bottom ends of the fourth guide plates (816), the side end faces of the third guide plates (815) and the side end faces of the fourth guide plates (817) are fixedly connected to the side end faces of the second guide plates (818), the inner walls of the first guide plates (814) are fixedly connected to the fourth guide plates (819) and the outer walls of the fourth guide plates (819) are fixedly connected to the fourth guide plates (818), the fourth guide plates (817) are respectively connected with the fixing rods (819), The fourth rubber block (816), the third guide plate (815) and the third rubber block (814) are in one-to-one correspondence, and the vertical center line of the first guide frame (802) and the vertical center line of the second guide frame (813) are located on the same vertical line.
  2. 2. The flow cytometer capable of improving particle resolution as described in claim 1, wherein the outer wall of the test tube (7) is attached to the inner wall of the placement frame (6), the top end surface of the supporting plate (10) is attached to the bottom end surface of the mounting plate (12), four second hydraulic rods (11) are uniformly distributed around the mounting plate (12), the second lenses (15) are symmetrically distributed on the front side and the rear side of the interior of the protective housing (1), the optical filters (16) are equidistantly distributed on the front side and the rear side of the interior of the protective housing (1), the optical filters (16) are in an inclined shape, the optical filters (16) are in one-to-one correspondence with the second detectors (24), the collecting box (26) is attached to the inner wall of the protective housing (1), the inner wall of the collecting box (26) is attached to the outer wall of the second sponge frame (28), and the sponge plates (29) are equidistantly distributed in the second sponge frame (28).
  3. 3. The flow cytometer capable of improving particle resolution as described in claim 1, wherein a first liquid pump (820) is fixedly mounted at the top side end of the first flow guiding frame (802), a first connecting pipe (821) is flange-connected to the first liquid pump (820), one end of the first connecting pipe (821) is connected to the top of the second flow guiding frame (813), the other end of the first connecting pipe (821) is located right above the placement frame (6), a second liquid pump (822) is fixedly mounted on the inner top end surface of the protective housing (1), a second connecting pipe (823) is flange-connected to the second liquid pump (822), one end of the second connecting pipe (823) is connected with a flow dividing frame (824), the flow dividing frame (824) is welded and fixed to the inner top end surface of the first flow guiding frame (802), flow dividing holes (825) are formed in a penetrating mode at the bottom of the flow dividing frame (824), the whole flow dividing frame (824) is annular, and the flow dividing holes (825) are distributed at the bottom of the flow dividing frame (824) in an equiangular mode.
  4. 4. A flow cytometer capable of improving particle resolution as described in claim 3, wherein the uniformity processing assembly (9) comprises a first storage tank (901), the first storage tank (901) is welded and fixed on an inner bottom end surface of a protective housing (1), a first liquid inlet pipe (902) is connected to the top of the first storage tank (901), a first electromagnetic valve (903) is mounted on the first liquid inlet pipe (902), a servo motor (904) is mounted and fixed on the top end surface of the first storage tank (901), a first conical gear (905) is welded and fixed on an output shaft of the servo motor (904), a second conical gear (906) is connected and meshed and connected on the first conical gear (905), a sleeve (907) is welded and fixed on the second conical gear (906), the sleeve (907) is rotationally connected to the middle portion inside the first storage tank (901), the top of the sleeve (907) is rotationally connected with the other end of the second (823) through a sealing bearing, a first rotary vane (908) is mounted on the sleeve (901), a first rotary vane (908) is connected with a first rotary vane (908) and a first rotary vane (908) is connected with a first rotary vane (908) is welded and fixed on a first rotary vane (908), one end of the first screw rod (911) is fixedly welded with a circular gear (912), tooth blocks (913) are welded on the inner bottom end surface of the first storage box (901) at equal angles, the tooth blocks (913) are meshed with the circular gear (912), the first conveying cylinders (910) are distributed in the first storage box (901) at equal angles, and the first conveying cylinders (910) are in one-to-one correspondence with the fixed pipes (909).
  5. 5. The flow cytometer capable of improving particle resolution as described in claim 1, wherein the side end of the positioning block (17) is provided with a clamping groove (18), a positioning frame (19) is welded and fixed on the bottom end surface of the mounting plate (12), a return spring (20) is welded and fixed on the side end surface of the positioning frame (19), a clamping rod (21) is welded and fixed on the other end of the return spring (20), the clamping rod (21) is slidably connected in the positioning frame (19), the end part of the clamping rod (21) is clamped and connected in the clamping groove (18), the clamping grooves (18) are symmetrically distributed on two sides of the positioning block (17), the clamping grooves (18) are in one-to-one correspondence with the clamping rods (21), and the positioning block (17) is slidably connected in the positioning frame (19).
  6. 6. A flow cytometer for increasing resolution of particles as described in claim 1, wherein: the self-cleaning component (25) comprises a fixed plate (2501), the fixed plate (2501) is welded and fixed on the inner wall of the protective shell (1), a second through hole (2502) is formed in the fixed plate (2501) in a penetrating mode, a first sponge frame (2503) is fixedly connected to the fixed plate (2501), a first conveying pipe (2504), a second conveying pipe (2505) and a third conveying pipe (2506) are fixedly connected to the fixed plate (2501), one end of the first conveying pipe (2504) is connected with the first sponge frame (2503), the other end of the first conveying pipe (2504) and one end of the third conveying pipe (2506) are connected with the second conveying pipe (2505), the other end of the third conveying pipe (2506) is connected with a second conveying cylinder (2507), the second conveying cylinder (2507) is fixedly connected in a second storage box (2512), the second storage box (2512) is fixedly welded on the side end face of the protective shell (1), a second screw rod (2508) is rotationally connected with the second conveying cylinder (2507), the length and the thickness of the first sponge frame (2503) are respectively larger than the diameter and the thickness of the first lens (14), the diameter of the second lens (15) and the diameter of the optical filter (16) are the same, the length of the second screw rod (2508) is larger than that of the second conveying cylinder (2507), and the second conveying cylinder (2507) is fixed at the middle part inside the second storage box (2512).
  7. 7. The flow cytometer capable of improving particle resolution as described in claim 6, wherein a coil (2509) is fixedly connected to the top end of the second spiral rod (2508), a traction rope (2510) is wound on the coil (2509), the traction rope (2510) penetrates through and is slidably connected to the top side end of the second storage box (2512) and the top side end of the protective casing (1), the end of the traction rope (2510) is fixedly connected to the top end surface of the mounting plate (12), a guide wheel (2511) is mounted on the inner wall of the protective casing (1), the traction rope (2510) is slidably connected to the guide wheel (2511), a second liquid inlet pipe (3) is connected to the top side end of the second storage box (2512), a limit frame (5) is fixedly welded on the inner bottom end surface of the second storage box (2512), and a spiral spring (2516) is fixedly welded on the inner wall of the limit frame (2515), and the bottom end of the spiral spring (2506) is fixedly welded on the bottom of the spiral rod (2516).
  8. 8. A method for using a flow cytometer capable of improving the resolution of particles, a flow cytometer for increasing resolution of particles as described in claim 1, comprising the steps of: s1, obtaining materials from cells, tissues or organisms, preparing single-cell suspension, placing the single-cell suspension in a test tube (7), and then placing the test tube (7) in a placing frame (6) in a protective shell (1); s2, before the first lens (14), the second lens (15) and each optical filter (16) are used, the self-cleaning assembly (25) is matched for automatic cleaning, and the sheath liquid to be used is uniformly stirred through the uniform treatment assembly (9); S3, pumping and conveying the cell suspension and the sheath liquid by using an adjustable flow chamber (8), and guiding the cell suspension to pass through a gradually narrowing channel so that the cell population forms single cells before passing through a laser (22) of a monitoring point; s4, each cell in the cell suspension moves on the laser beam emitted by the laser (22), the first lens (14) and the first detector (23) are matched to analyze each cell in the sample individually, when each cell passes through the laser beam, the laser beam is scattered in multiple directions, and the laser beams scattered in multiple directions are collected and detected by the matching of the optical filters (16) and the corresponding second detectors (24).

Description

Flow cytometer capable of improving particle resolution and application method thereof Technical Field The invention relates to the field of cell detection, in particular to a flow cytometer capable of improving particle resolution and a use method thereof. Background The flow cytometer is a technique for cell analysis, which is widely used in the fields of biomedical research, clinical diagnosis, biopharmaceutical and the like, and the working principle is that single cells are suspended in liquid and irradiated by laser or other light sources, and the photoelectric detector is utilized to analyze the characteristics of the cells, the flow cytometer is a powerful tool, can help researchers and clinicians to understand the characteristics and functions of the cells deeply, and has important significance in basic research and clinical application. The existing flow cytometer has some problems in the actual working process, for example, the flow cytometer with the publication number of CN116698710A can adjust the internal temperature and humidity environment of equipment, but in the actual working process, the flow cytometer can not conveniently adjust the diameter of a flow channel according to different types of samples (such as cell suspension, particles and the like), so that the flow characteristic of the samples can be influenced, the particle resolution is lower, the detection result is inaccurate, in the actual working process, each optical filter which needs to be used can not be automatically cleaned, and the proper optical filter can not be conveniently replaced, so that the signal capturing performance is poor, and the accuracy of data is influenced. Disclosure of Invention The present invention has been developed in view of the problems associated with prior flow cytometers and methods of using the same that can improve particle resolution. According to the technical scheme, the flow cytometer capable of improving particle resolution comprises a protective shell, a first lens, a second lens and an optical filter, wherein a partition plate is fixedly welded in the protective shell, a first door plate and a second door plate are installed on the protective shell, a first hydraulic rod is installed at the bottom end inside the protective shell, a placing frame is fixedly connected to the top end of the first hydraulic rod, a test tube is arranged in the placing frame, an adjustable flow chamber is installed in the protective shell, a uniform processing assembly is installed in the protective shell, supporting plates are fixedly welded on the inner wall of the protective shell and the side end faces of the partition plate, a second hydraulic rod is installed at the top end inside the protective shell, a mounting plate is fixedly connected to the bottom end of the second hydraulic rod, a first through hole is formed in the mounting plate in a penetrating mode, a positioning block is fixedly installed at the side end face of the partition plate, a first detector is fixedly connected to the top end face inside the protective shell, a second detector is arranged in the side face inside the protective shell, a magnetic sponge collecting box is fixedly connected to the side face inside the protective shell, a magnetic collecting box is fixedly connected to the side face of the magnetic collecting box, and a magnetic collecting box is fixedly connected to the side face of the magnetic collecting box. As a preferable scheme of the invention, the outer wall of the test tube is attached to the inner wall of the placement frame, the top end surface of the supporting plate is attached to the bottom end surface of the mounting plate, four second hydraulic rods are arranged around the mounting plate and symmetrically distributed on the front side and the rear side of the inside of the protective shell, the optical filters are equidistantly distributed on the front side and the rear side of the inside of the protective shell and are in an inclined shape, the optical filters are in one-to-one correspondence with the second detectors, the collecting box is attached to the inner wall of the protective shell, the inner wall of the collecting box is attached to the outer wall of the second sponge frame, and the sponge plates are equidistantly distributed in the second sponge frame. The adjustable flow chamber comprises a supporting plate, the supporting plate is fixedly welded on the side end face of a partition plate, a first flow guiding frame is fixedly welded on the supporting plate, a fixing frame is fixedly welded at the bottom of the first flow guiding frame, a threaded rod is connected to the top of the fixing frame in a threaded mode, a connecting plate is rotatably connected to the bottom end of the threaded rod, a push rod is hinged to the bottom end face of the connecting plate, a first flow guiding plate is hinged to the bottom end face of the push rod, a telescopic sleeve rod is fixedly welded on the side