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CN-119236146-B - Magnetic particle-protein fiber dressing and preparation method and application thereof

CN119236146BCN 119236146 BCN119236146 BCN 119236146BCN-119236146-B

Abstract

The invention belongs to the technical field of scar-free skin injury repair, and particularly relates to a magnetic particle-protein fiber dressing, and a preparation method and application thereof. The invention coats magnetic nano particles, the obtained coated magnetic nano particles are mixed with water-soluble copper salt and water for carrying out copper ion loading, the obtained coated magnetic nano particles loaded with copper ions are mixed with functional protein and buffer solution for incubation, the obtained magnetic microsphere-protein composite solution is placed in an external magnetic field for magnetic attraction, and then directional assembly is carried out in the external magnetic field, so as to obtain the magnetic particle-protein fiber assembly. The magnetic particle-protein fiber assembly and the medical dressing modified by the magnetic particle-protein fiber coating can realize the scar-free rapid repair of wounds and restore the integrity of skin functions, and the preparation method is simple, thereby having important clinical application value and market prospect.

Inventors

  • WANG DAILONG
  • HE CHENLONG

Assignees

  • 同济大学

Dates

Publication Date
20260508
Application Date
20240930

Claims (5)

  1. 1. Use of a magnetic particle-protein fiber dressing for the preparation of a medical device for scar-free wound healing, characterized in that the preparation method of the magnetic particle-protein fiber dressing comprises the steps of: Coating the magnetic nano particles to obtain coated magnetic nano particles, wherein the coating material of the coated magnetic nano particles is negatively charged polymer, and the negatively charged polymer is polydopamine; Mixing the coated magnetic nano-particles, water-soluble copper salt and water for carrying out copper ion loading to obtain coated magnetic nano-particles loaded with copper ions; mixing and incubating the copper ion-loaded coated magnetic nanoparticles, functional proteins and buffer solution to obtain a magnetic microsphere-protein complex solution, wherein the functional proteins comprise one or more of fibrinogen, albumin, collagen, fibronectin, laminin and cytokines; Placing the magnetic microsphere-protein complex solution in an external magnetic field to perform magnetic attraction to obtain a magnetic composite microsphere intermediate assembly; Transferring the heavy suspension of the magnetic composite microsphere intermediate assembly to the surface of a substrate, and then carrying out directional assembly under the control of a constant externally-applied magnetic field to obtain the magnetic particle-protein fiber dressing, wherein the magnetic field strength of the constant externally-applied magnetic field used for directional assembly is 0.5-1 mT, and the time of the directional assembly is 10-30 min.
  2. 2. The use of claim 1, wherein the magnetic nanoparticles comprise one or more of Fe 3 O 4 nanoparticles, γ -Fe 2 O 3 nanoparticles, coFe 2 O 4 nanoparticles, and prussian blue nanoparticles; The particle size of the magnetic nano particles is 1-300 nm.
  3. 3. The use according to claim 1, wherein the coating is at room temperature for a period of time not less than 8 h; The copper ion loading time is more than or equal to 12 h; the incubation time is 10-20 min; The external magnetic field used by magnetic attraction is provided by a magnetic frame, and the time of the magnetic attraction is 2-5 min.
  4. 4. The use according to claim 1, wherein the substrate comprises one or more of a methacryloylated gelatin hydrogel, a methacryloylated hyaluronic acid hydrogel, a sodium alginate hydrogel, and a PEG hydrogel.
  5. 5. The use according to claim 1 or 4, wherein the magnetic particle-protein fiber dressing comprises a substrate and a magnetic particle-protein fiber assembly disposed on a surface of the substrate; The magnetic particle-protein fiber assembly comprises colloid fibers which are arranged in an oriented mode, wherein the colloid fibers comprise a framework and functional proteins covered on the surface of the framework, and the framework is formed by coated magnetic nano particles loaded with copper ions.

Description

Magnetic particle-protein fiber dressing and preparation method and application thereof Technical Field The invention belongs to the technical field of scar-free skin injury repair, and particularly relates to a magnetic particle-protein fiber dressing, and a preparation method and application thereof. Background The skin is used as the strongest organ of human body, is the first barrier of human body, and can resist external injury or pathogen microorganism invasion. However, in the natural world, the skin is relatively fragile, and is damaged by high temperature, scratch, biochemical substances and other factors, especially chronic wounds, burns and skin wound infection, and the skin is required to be subjected to hard long-term treatment, so that the skin becomes an important cause for seriously damaging the physical and mental health of people and reducing the life and life quality of people. Clinical wound healing problems are widespread, ranging from "under-healing" (i.e. chronic/non-healing wounds) to "over-healing" (i.e. scarring/fibrosis). Wound healing includes the classical three phases, inflammation, proliferation, remodeling, and new insight shows that the overlapping or parallel (Baron J M,Glatz M,Proksch E.Optimal Support of Wound Healing:New Insights[J].Dermatology,2020,236(6):593-600.). of these three phases in time and space of the wound broadly speaking, a deregulation or interruption of wound healing at either phase results in healing beyond normal limits. In a narrow sense, they represent both ends of a wound healing time curve. On the one hand, the wound is slow to heal, and in the case of stress/tissue ischemia, bacterial inflammation or immune dysfunction (usually caused by systemic causes such as diabetes or atherosclerosis), the wound may not heal at all. However, chronic unhealed wounds remain open to the outside, which is highly susceptible to complications, infections that may develop from the skin to the underlying structure, requiring surgical intervention and even amputation. Another aspect is scarring after excessive healing. At present, implantable medical devices for tissue repair are rapidly developed, and various biological materials such as antibacterial gauze, cotton balls, hydrogel films, microspheres, nanofibers and the like have been developed. Although the implantable biomaterials described above are generally capable of promoting wound healing, scar formation due to implantable biomaterials and rapid injury repair remains a challenge to be addressed. Because implants cause complex immune responses in the body and promote proliferation of fibroblasts and collagen deposition in large amounts, fibrosis occurs, which is weaker in tensile strength than normal skin and loses the integrity of skin function. In conclusion, the current tissue repair materials cannot realize the rapid repair of the skin without scars. Disclosure of Invention The invention aims to provide a magnetic particle-protein fiber assembly, a preparation method and application thereof, a magnetic particle-protein fiber dressing, and a preparation method and application thereof, wherein the magnetic particle-protein fiber assembly and the magnetic particle-protein fiber dressing provided by the invention can realize the rapid repair of wounds without scars and restore the integrity of skin functions; the preparation method is simple, and has important clinical application value and market prospect. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a preparation method of a magnetic particle-protein fiber assembly, which comprises the following steps: coating the magnetic nano particles to obtain coated magnetic nano particles, wherein the coating material of the coated magnetic nano particles is a negatively charged polymer; Mixing the coated magnetic nano-particles, water-soluble copper salt and water for carrying out copper ion loading to obtain coated magnetic nano-particles loaded with copper ions; Mixing the coated magnetic nano particles loaded with copper ions, functional protein and buffer solution for incubation to obtain a magnetic microsphere-protein complex solution; Placing the magnetic microsphere-protein complex solution in an external magnetic field to perform magnetic attraction to obtain a magnetic composite microsphere intermediate assembly; and carrying out directional assembly on the heavy suspension of the magnetic composite microsphere intermediate assembly under the control of a constant externally-applied magnetic field to obtain the magnetic particle-protein fiber assembly. Preferably, the magnetic nanoparticles comprise one or more of Fe 3O4 nanoparticles, γ -Fe 2O3 nanoparticles, coFe 2O4 nanoparticles, and prussian blue nanoparticles; the particle size of the magnetic nano particles is 1-300 nm. Preferably, the negatively charged polymer comprises one or more of polydopamine, chitosan and hydroxym