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CN-119368287-B - Device for identifying root of flare and mixed and fake product thereof

CN119368287BCN 119368287 BCN119368287 BCN 119368287BCN-119368287-B

Abstract

The invention belongs to the technical field of identification of medicinal plants and traditional Chinese medicines, and discloses a device for identifying a flare root and a mixed product thereof, which comprises a liquid nitrogen ball mill, a DNA extraction kit and a PCR amplification instrument which are sequentially connected, wherein the liquid nitrogen ball mill is connected with a variable speed motor, the device also comprises a sealing assembly, the sealing assembly comprises a first permanent magnet and a sealing ball, the first permanent magnet and the sealing ball repel each other, the first permanent magnet is slidingly arranged in a first through groove, the sealing ball is slidingly arranged in a wedge-shaped through groove and is connected with a tension spring, the free end of the tension spring is fixedly connected with the wedge-shaped through groove, the first through groove and the wedge-shaped through groove are respectively provided with a sliding groove, the first permanent magnet and the sealing ball are respectively and slidingly connected with the sliding groove, and the diameter of the sealing ball is not smaller than the diameter of an opening of the wedge-shaped through groove. The invention solves the problem that the traditional liquid nitrogen ball mill can not adjust the liquid nitrogen supplementing amount according to the ball milling rotating speed.

Inventors

  • WANG JIANGRUI
  • ZHAO JIANQUAN
  • WANG XUEZHENG
  • ZENG QIN
  • ZHAO JIANGYI

Assignees

  • 重庆市药研院制药有限公司

Dates

Publication Date
20260508
Application Date
20241022

Claims (10)

  1. 1. The device for identifying the spark root and the mixed and false product thereof comprises a liquid nitrogen ball mill (1), a DNA extraction kit and a PCR amplification instrument which are sequentially connected, and is characterized in that the liquid nitrogen ball mill (1) is connected with a variable speed motor, a first through groove (111) is formed in a feed inlet (11) of the liquid nitrogen ball mill (1), the first through groove (111) is communicated with the feed inlet (11), a wedge-shaped through groove (121) is formed in a bearing seat (12) of the liquid nitrogen ball mill (1), the wedge-shaped through groove (121) is communicated with the first through groove (111), the wedge-shaped through groove (121) is communicated with a liquid nitrogen tank through a pipeline, the device further comprises a sealing assembly (2), the sealing assembly comprises a first permanent magnet (21) and a sealing ball (22), the magnetic phase of the first permanent magnet (21) and the sealing ball (22) is repelled by sliding in the first through groove (111), the sealing ball (22) is slidingly arranged in the wedge-shaped through groove (121), and the wedge-shaped through groove (121) is connected with a tension spring (23), and the free end of the wedge-shaped through groove (23) is fixedly arranged in the pipeline (121).
  2. 2. The device for identifying the root of a torch and the mixed and false product thereof according to claim 1, wherein the first through groove (111) and the wedge-shaped through groove (121) are respectively provided with a sliding groove, and the first permanent magnet (21) and the sealing ball (22) are respectively connected with the sliding grooves in a sliding way.
  3. 3. The device for discriminating a root of a torch and a mixed product thereof according to claim 1 wherein said sealing ball (22) has a diameter not smaller than the diameter of the opening of the wedge-shaped through groove (121).
  4. 4. The device for identifying the root of a torch and its mixed and false products according to claim 1, wherein the width of said first permanent magnet (21) is smaller than the inner diameter of the first through groove (111).
  5. 5. The device for identifying the root of a flare and a fake product thereof according to claim 4, wherein the first through groove (111) is an annular through groove, the first permanent magnets (21) are two semicircular permanent magnets which are symmetrically arranged, and the end surfaces of the two first permanent magnets (21) are mutually attached.
  6. 6. The device for identifying the mixture of the turkey roots and the mixture of the turkey roots, as set forth in claim 1, is characterized in that the ball milling tank (13) of the liquid nitrogen ball mill (1) is provided with an inner cylinder (131) and an outer cylinder (132), the inner cylinder (131) is coaxially connected with the outer cylinder (132), a cavity (133) is arranged between the inner cylinder (131) and the outer cylinder (132), the inner cylinder (131) is provided with a second through groove, the device further comprises a screening assembly (3), the screening assembly (3) comprises a baffle plate (31) and a screen plate (32), the baffle plate (31) is fixedly connected with one end of the screen plate (32), the baffle plate (31) and the screen plate (32) are slidably arranged in the second through groove, the other end of the screen plate (32) is connected with a spring (33), the free end of the spring (33) is fixedly connected with the inner cylinder (131), the screen plate (32) is connected with a second permanent magnet (34), and an electromagnet (35) is arranged in the inner cylinder (131) and is used for electrically attracting the second permanent magnet (34).
  7. 7. The device for identifying the root of a torch and the mixed and false products thereof according to claim 6, wherein the outer cylinder (132) is provided with a discharge port, the discharge port is communicated with the cavity (133), and the discharge port is provided with a baffle plate.
  8. 8. The device for identifying the roots of the firearms and the mixed products thereof according to claim 6, wherein the inner cylinder (131) is internally provided with a guide groove, and the baffle plate (31) and the sieve plate (32) are both in sliding connection with the guide groove.
  9. 9. The device for distinguishing a root of a torch from its mixed products according to claim 6, wherein said baffle (31) and said screen (32) have the same radius of curvature as the inner cylinder (131).
  10. 10. The device for identifying the root of a flare and a mixed product thereof according to claim 6, wherein the screening assembly (3) further comprises a driving unit (36), the driving unit (36) comprises a touch switch (361) and a return spring (362), the return spring (362) is fixedly connected with the first through groove (111), the free end of the return spring (362) is fixedly connected with the first permanent magnet (21), the touch switch (361) is arranged in the first through groove (111), the first permanent magnet (21) is used for abutting against the touch switch (361), and the touch switch (361) is electrically connected with the electromagnet (35).

Description

Device for identifying root of flare and mixed and fake product thereof Technical Field The scheme belongs to the technical field of medical plants and traditional Chinese medicine identification, and particularly relates to a device for identifying the root of a torch and a mixed and fake product thereof. Background Referring to the background technology of the prior publication No. CN113862390A, the root of the Chinese torch and the common threewingnut root belong to the common threewingnut root of Celastraceae, other medicinal materials such as the Chinese torch root medicinal material and the common threewingnut root are often used by some suppliers on the market, and medicinal parts are root parts, and the Chinese torch root medicinal materials such as the common threewingnut root, the common threewingnut root and the common threewingnut root are difficult to distinguish due to the factors such as extremely similar appearance, limited knowledge of an identifier and the like, so that the market confusion and the medication accuracy and safety are seriously influenced. The method for extracting genome DNA of the spark root and the mixed and imitative product thereof comprises the steps of scrubbing the surfaces of the spark root and the mixed and imitative product thereof respectively by adopting 75% ethanol, respectively crushing the surfaces into fine powder, respectively taking 60mg of the spark root and the mixed and imitative product thereof, respectively adding liquid nitrogen, respectively grinding for 5min for 30 times/s by using a full-automatic rapid ball mill to respectively obtain samples of the spark root and the mixed and imitative product thereof, respectively extracting total DNA of the samples of the spark root and the mixed and imitative product thereof according to the description of the kit by using an optimized plant DNA extraction kit, and obtaining the DNA template of the respectively extracted spark root and the mixed and imitative product thereof. Therefore, extracting the genome of the flare root and its mixed and imitative products requires grinding the flare root pieces and mixed and imitative products using a liquid nitrogen ball mill. See a frozen liquid nitrogen type ball mill with the prior publication number CN109351444A, which comprises a frame, a low temperature resistant adapter and a grinding tank, wherein a supporting seat and a driving piece for driving the grinding tank to rotate are arranged on the frame, the grinding tank is arranged on the supporting seat, one end of the low temperature resistant Wen Zhuaijie head is detachably connected with the grinding tank, and the other end of the low temperature resistant Wen Zhuaijie head is communicated with the frozen liquid nitrogen tank. According to the ball mill, the frozen liquid nitrogen tank is communicated with the milling tank through the low-temperature-resistant adapter, so that liquid nitrogen is intermittently or continuously supplied to the milling tank to enable the inside of the milling tank to be in a low-temperature environment, and the milling tank can crush or grind materials after being frozen and fragile. Although the ball mill can intermittently or continuously supply liquid nitrogen to the milling tank, when the ball mill grinds materials, a large amount of heat energy is generated due to continuous collision and friction between the grinding balls in the milling tank and the materials and the tank wall under high-speed operation, so that the temperature is increased, and the continuous friction and collision can gradually increase the temperature in the tank even though the liquid nitrogen can keep an initial low-temperature environment, especially when the rotating speed is higher, the heat generation rate is faster. And the excessive temperature can lead to hydrogen bond breakage of materials, such as DNA molecules in medicinal materials, so that the double-helix structure is unwound, thereby reducing the integrity of DNA and affecting the accuracy of subsequent genome analysis and identification results. Thus, the liquid nitrogen make-up and the ball milling rotational speed are interactive with the temperature in the ball milling tank. The higher the rotation speed, the more frequent the collision and friction between the grinding balls in the ball milling tank and the materials and the tank wall, resulting in temperature rise, while the liquid nitrogen supplement is used for maintaining the low temperature environment in the grinding tank. Therefore, the supplementing amount of liquid nitrogen needs to be adjusted according to the change of the rotating speed so as to ensure that the temperature in the grinding tank is kept in a proper range, thereby avoiding the breakage of DNA molecular hydrogen bonds in the medicinal materials caused by the overhigh temperature and ensuring the accuracy of the subsequent genome analysis and identification results. Disclosure of Invention The purpose of t