CN-119372055-B - Nucleic acid POCT detector

Abstract

The invention discloses a nucleic acid POCT detector. The detection device of the detector comprises a consumable bin, a mobile mechanical arm module, a nucleic acid extraction and purification module and an amplification detection module, wherein the mobile mechanical arm module is provided with a magnetic rod position for installing a magnetic rod, a suction nozzle position and a first clamping jaw, and the amplification detection module is used for amplifying and detecting nucleic acid in a sample to be detected in real time. The invention provides a detector capable of automatically completing nucleic acid detection. The detector is provided with a plurality of detection channels, can detect one to a plurality of samples at the same time, adopts the technical means of rapid temperature rise and reduction, rapid and accurate constant temperature, photoelectric signal noise removal and amplification and the like, achieves high integration and high automation, reduces the volume to the limit, can automatically complete the detection of 1 to 21 pathogen nucleic acids in the whole process from the samples to the results within 30 minutes, and can send the results. Compared with the traditional fluorescence PCR method, the method has the advantages that the detection time is greatly shortened, the convenience is greatly improved, and the requirements on professionals and places are very low, so that the application range is very wide.

Inventors

• LIU HELU
• ZHAO LIJUN
• Huang Xuanmei
• LIU YIXUAN
• CHEN SHUANGLI
• CAI ZIYAN

Assignees

• 深圳市中西医结合医院

Dates

Publication Date

20260508

Application Date

20241105

Claims (6)

1. 1. The utility model provides a nucleic acid POCT detector which characterized in that, include the plummer and establish with at least one detection device on the plummer, detection device all includes: The consumable bin is arranged on the bearing table and is provided with a reagent groove accommodating position for accommodating a reagent groove, a sample Guan Rong position for accommodating a sample tube and a reaction tube Rong Zhiwei for accommodating a reaction tube, the sample Guan Rong position is formed by a second clamping jaw arranged on the bearing table, the second clamping jaw is connected with a fifth driving mechanism in a driving connection manner, and the fifth driving mechanism is used for driving the second clamping jaw to open and close; The mobile mechanical arm module is arranged on the bearing table and is provided with a magnetic rod position for installing a magnetic rod, a suction nozzle position for installing a suction nozzle and a first clamping jaw for clamping the reaction tube and/or the sample tube and carrying out opening/closing cover treatment on the reaction tube and/or the sample tube, the mobile mechanical arm module comprises a sliding rail, a sliding block, a second driving mechanism, a third driving mechanism, a fourth driving mechanism, a magnetic rod installation block, a sixth driving mechanism, a suction nozzle installation block and a seventh driving mechanism, the sliding block is arranged on the sliding rail in a sliding manner and is in driving connection with the second driving mechanism, the second driving mechanism is used for driving the sliding block to slide, the first clamping jaw is arranged on the sliding block and is in driving connection with the third driving mechanism and the fourth driving mechanism respectively, the third driving mechanism is used for driving the first clamping jaw to open/close, the magnetic rod position is arranged on the magnetic rod installation block, the magnetic rod installation block is arranged on the sliding block in a lifting manner and is connected with the seventh driving mechanism in a lifting manner, the magnetic rod installation block is arranged on the sliding block and can be lifted and is connected with the sixth driving mechanism in a lifting manner, and the suction nozzle is arranged on the suction nozzle installation block in a lifting manner and can be lifted and lifted The amplification detection module is arranged on the bearing table and provided with a detection groove for accommodating the reaction tube, and the amplification detection module is used for amplifying and detecting the nucleic acid in the sample to be detected in real time.
2. 2. The nucleic acid POCT detector of claim 1, wherein the detection device further comprises a first drive mechanism drivingly connected to the consumable cartridge and configured to drive the consumable cartridge into and out of sliding relation to the carrier.
3. 3. The nucleic acid POCT detector of claim 1, wherein a movable flag is provided at a notch of the detection slot, and the first jaw is further configured to clamp or push against the movable flag and move the movable flag under the drive of the second drive mechanism.
4. 4. The nucleic acid POCT detector of claim 1, wherein the consumable cartridge further comprises a consumable rack mounted on the carrier and provided with a plurality of magnet bar slots for placing the magnet bars and a plurality of suction nozzle slots for placing the suction nozzles.
5. 5. The nucleic acid POCT detector of claim 1, wherein the detection device further comprises a heating means provided at the bottom of the reagent vessel for performing thermal amplification of the sample to be detected.
6. 6. The nucleic acid POCT detector of any one of claims 1 to 5, further comprising a code scanner disposed on the carrier and disposed adjacent to the sample Guan Rong, the code scanner being configured to identify sample information of the sample tube.

Description

Nucleic acid POCT detector Technical Field The invention relates to the technical field of nucleic acid detection instruments, in particular to a nucleic acid POCT detector. Background In the prior art, a detection person generally sucks a sample by using a suction nozzle, and then drops the sample into a reaction tube, and a PCR amplification detector is used for detecting nucleic acid. However, this detection method relies largely on manual operation, which is inefficient and may result in inaccurate detection results due to improper operation. Disclosure of Invention The invention mainly aims to provide a nucleic acid POCT detector, which aims to realize the automation of nucleic acid detection, improve the detection efficiency and improve the accuracy of detection results. To achieve the above object, the present invention provides a nucleic acid POCT detector, comprising at least one detection device, each of the detection devices comprising: A carrying platform; The consumable bin is arranged on the bearing table and is provided with a reagent groove accommodating position for accommodating a reagent groove, a sample Guan Rong position for accommodating a sample tube and a reaction tube Rong Zhiwei for accommodating a reaction tube; a mobile mechanical arm module arranged on the bearing table and provided with a magnetic rod position for installing a magnetic rod, a suction nozzle position for installing a suction nozzle and a first clamping jaw for clamping and opening/closing the reaction tube and/or the sample tube, and The amplification detection module is arranged on the bearing table and provided with a detection groove for accommodating the reaction tube, and the amplification detection module is used for amplifying and detecting the nucleic acid in the sample to be detected in real time. Optionally, the nucleic acid POCT detector further includes a first driving mechanism, where the first driving mechanism is in driving connection with the consumable cartridge and is used to drive the consumable cartridge to slide in and out relative to the bearing table. Optionally, the mobile mechanical arm module comprises a sliding rail, a sliding block, a second driving mechanism, a third driving mechanism and a fourth driving mechanism, wherein the sliding block is slidably arranged on the sliding rail and is in driving connection with the second driving mechanism, the second driving mechanism is used for driving the sliding block to slide, the first clamping jaw is arranged on the sliding block and is in driving connection with the third driving mechanism and the fourth driving mechanism respectively, the third driving mechanism is used for driving the first clamping jaw to open and close, and the fourth driving mechanism is used for driving the first clamping jaw to rotate so as to open/close the cover. Optionally, the sample Guan Rong is set and is formed by a second clamping jaw disposed on the carrying platform, and the second clamping jaw is connected with a fifth driving mechanism in a driving manner, and the fifth driving mechanism is used for driving the second clamping jaw to open and close. Optionally, a movable light blocking member is arranged at the notch of the detection groove, and the first clamping jaw is further used for clamping or pushing the movable light blocking member and moving the movable light blocking member under the drive of the second driving mechanism. Optionally, the mobile mechanical arm module further comprises a magnetic rod installation block and a sixth driving mechanism, wherein the magnetic rod is arranged on the magnetic rod installation block, the magnetic rod installation block is arranged on the sliding block in a lifting manner and is in driving connection with the sixth driving mechanism, and the sixth driving mechanism is used for driving the magnetic rod installation block to do lifting motion. Optionally, the mobile mechanical arm module further includes a suction nozzle mounting block and a seventh driving mechanism, the suction nozzle is located on the suction nozzle mounting block, the suction nozzle mounting block is liftably disposed on the slider and is in driving connection with the seventh driving mechanism, and the seventh driving mechanism is used for driving the suction nozzle mounting block to perform lifting motion. Optionally, the consumable bin further comprises a consumable rack, wherein the consumable rack is installed on the bearing table and is provided with a plurality of magnetic rod slots for placing the magnetic rods and a plurality of suction nozzle slots for placing the suction nozzles. Optionally, the nucleic acid POCT detector further comprises a heating device, wherein the heating device is arranged at the bottom of the reagent tank and is used for heating and amplifying the sample to be detected. Optionally, the nucleic acid POCT detector further includes a code scanner, where the code scanner is disposed on the carrying platf