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CN-119372111-B - Bacillus albosis LZ01 and application thereof in preparation of preparation for preventing and treating brown planthopper of rice

CN119372111BCN 119372111 BCN119372111 BCN 119372111BCN-119372111-B

Abstract

The invention discloses bacillus alzhi LZ01 and application thereof in preparation of a preparation for preventing and controlling brown planthoppers of rice, and provides bacillus alzhi LZ01 which can improve the resistance/tolerance of pest-sensing rice varieties to brown planthoppers after being used for treating rice culture matrixes, and has good application prospects.

Inventors

  • WANG WEIXIA
  • HU ZHENGHAO
  • LAI FENGXIANG
  • FU QIANG
  • WAN PINJUN

Assignees

  • 中国水稻研究所

Dates

Publication Date
20260508
Application Date
20241121

Claims (9)

  1. 1. Bacillus aryabhattai (Bacillus aryabhattai) LZ01 is preserved in China center for type culture Collection, with preservation number CCTCC NO: M20241485, and preservation date 2024, month 07, and 4, address Chinese university of Wuhan.
  2. 2. Use of bacillus arvensis LZ01 according to claim 1 for preparing a formulation for controlling brown planthopper of rice.
  3. 3. The use according to claim 2, wherein the preparation is prepared from bacterial liquid of bacillus aryabhattai LZ01 expanded culture and rice nutrient solution.
  4. 4. The use according to claim 3, wherein the rice nutrient solution is Yoshida rice nutrient solution.
  5. 5. The use according to claim 3, wherein the concentration of the bacterial cells in the formulation is 0.5 to 1 x10 8 CFU/mL.
  6. 6. The application of the bacillus subtilis LZ01 as set forth in claim 3, wherein the bacterial liquid is prepared by inoculating bacillus aryabhattai LZ01 into an LB plate medium, culturing at 37 ℃ for 24 hours for activation, picking up single bacterial colony after activation, inoculating the single bacterial colony into the LB liquid medium, culturing at 37 ℃ for 24 hours to obtain a seed liquid, inoculating the seed liquid into the LB medium with the inoculum size of 1-3% of volume concentration, and culturing at 37 ℃ until the OD600 is 0.6-0.8 to obtain the bacterial liquid.
  7. 7. The use according to claim 2, wherein said formulation is added to a sterilized cultivation substrate and the rice seeds are sown, effective to increase the resistance of the rice plant to brown planthoppers.
  8. 8. The use according to claim 7, wherein the cultivation substrate comprises sand and soil.
  9. 9. The use according to claim 7, wherein the formulation is used in an amount of 0.5-1.0mL/g based on the weight of the cultivation substrate.

Description

Bacillus albosis LZ01 and application thereof in preparation of preparation for preventing and treating brown planthopper of rice Field of the art The invention relates to the field of agricultural microorganisms, in particular to bacillus arvensis with a function of improving brown planthopper resistance of rice and application thereof. (II) background art Bacillus has strong stress resistance, rapid colonization capability, strong nutrition competition, plant resistance induction and other capabilities, and many reports are made on plant disease control, growth promotion and other aspects. For example, bacillus subtilis can stably survive and reproduce on the surfaces of soil and plant roots and leaves, can secrete antibiotics and hormone for stimulating plant growth, and can promote parasitic plants to generate disease resistance and resist pathogenic diseases. Some bacillus is reported to have good control effects on tomato bacterial wilt, wheat scab, watermelon fusarium wilt, tobacco bacterial wilt, cotton fusarium wilt, rice sheath blight and the like at home and abroad, and the yield and income of the product are promoted. The bacillus thuringiensis can produce protein with insecticidal activity, is widely applied to plant pest control, and has good control effect on lepidoptera pest larvae, such as cabbage caterpillar, rice bract, inchworm, pine moth, tobacco budworm, corn borer, cotton bollworm, rice leaf roller, straw moth, cutworm and the like. Bacillus aryabhattai (Bacillus aryabhattai) is widely distributed and found in soil, animal intestinal tracts, plant rhizosphere and the like. The strain is mainly applied to material degradation, natural product synthesis, plant growth promotion, plant stress resistance improvement and the like. The bacillus arvensis has stronger environmental adaptability, is easy to survive and colonize in farmland soil, plant habitats and the like, and has the characteristics of environmental friendliness, safety, no toxicity and the like. There are also some reports and patents on agricultural research. Patents CN201610036163.4, CN202111203354, CN202111159072.7, CN202210026480 describe several strains of bacillus aryabhattai. The strain can produce auxin IAA, siderobore, ammonia, cellulase, protease and the like, has the capability of dissolving organic phosphorus compounds, has obvious growth promoting effect on plants such as tomatoes, potatoes and the like, can effectively inhibit the growth of pathogenic bacteria of potato scab, has application value in biological control and saline-alkali soil improvement of potato scab, has activity of inhibiting pathogenic bacteria such as septoria pinnatifida, rhizoctonia solani, poplar rot germ, apple ring rot germ, tomato gray mold germ, tobacco brown spot germ, watermelon soft rot germ, tobacco black shank rice blast, fusarium wilt, potato scab, trichoderma reesei, aflatoxin and the like, has related patents of the bacillus albopictus with the functions of degrading polyvinyl plastics (CN 20161136508. X) and the like, has reports that the bacillus albopictus can degrade residues and produce glucose and produce the activity of preventing and controlling the pathogenic bacteria, has the function of protecting and controlling the bacillus albopictus with the quality of the soil, and the mineral substance of the polysaccharide from being well-degrading the soil of the soil-borne nematode, such as the bacillus Czotobacter, the polysaccharide is produced by the bacillus Czotobacter with the functions of the strain with the quality of the strain is well as the strain is capable of degrading the mineral substance of producing the mineral substance of the strain with the mineral substance of the strain to produce the strain to the soil. Therefore, the bacillus aryabhattai strain is rich in diversity and has various functions and different application values. The related studies and applications of Guan Ashi bacillus are still not deep and extensive, and in particular, screening and applications of bacillus arvensis strains with specific functions are few. Rice is one of the most important grain crops in China, and the total annual rice yield reaches 2100 hundred million kilograms and accounts for more than 40% of the total grain yield. However, rice is stressed by various external environmental factors at various stages of growth and development. It has been reported that hundreds of insects can feed on rice. Brown planthoppers (NILAPARVATA LUGENS stal.) are monophagic rice pests and belong to the family of the plant hoppers of the order Hemiptera (Hemiptera: DELPHACIDAE). Brown planthoppers suck juice in the phloem of rice through a mouth needle and transmit plant viruses. Mild damage to brown planthoppers results in reduced vigor and yield of rice plants. When the pest amount is large, the rice basal part is blackened, rotten and smelly, the rice is agglomerated in a short period, the dead stems are fallen in slices, and the parti