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CN-119410662-B - Application of OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice

CN119410662BCN 119410662 BCN119410662 BCN 119410662BCN-119410662-B

Abstract

The invention relates to application of an OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice, and a rice breeding method for synergistically improving the drought resistance and the nitrogen utilization efficiency of the rice, which comprises the step of improving the expression level of the OsWOX13 gene in the rice. The invention digs the multifunctional function of OsWOX13 in regulating and controlling the drought stress response and nitrogen utilization efficiency of rice, provides a new view for creating high-quality rice resources, and provides a molecular basis for the future collaborative cultivation of rice with drought tolerance and high nitrogen utilization efficiency.

Inventors

  • ZHAO YU
  • Tan Mingfang
  • ZHOU DAOXIU

Assignees

  • 华中农业大学

Dates

Publication Date
20260508
Application Date
20241216

Claims (6)

  1. The application of the OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice is disclosed, wherein the amino acid sequence coded by the OsWOX13 gene is shown as SEQ ID NO. 2.
  2. 2. The use according to claim 1, wherein the nucleotide sequence of the OsWOX13 gene is shown in SEQ ID No. 1.
  3. 3. A rice breeding method for synergistically improving drought resistance and nitrogen utilization efficiency of rice is characterized by comprising the step of improving the expression level of an OsWOX13 gene in the rice, wherein the amino acid sequence coded by the OsWOX13 gene is shown as SEQ ID NO. 2.
  4. 4. The method according to claim 3, wherein the expression level of the OsWOX13 gene is increased by transferring the OsWOX13 gene into a super-expression cassette.
  5. 5. The method according to claim 3, wherein the expression level of the OsWOX13 gene is increased by introducing the OsWOX13 gene having a higher expression level into rice by hybridization.
  6. 6. The method according to claim 3, wherein the nucleotide sequence of the OsWOX13 gene is shown in SEQ ID NO. 1.

Description

Application of OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice Technical Field The invention relates to the field of rice molecular breeding, and relates to application of an OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice, and a rice breeding method for synergistically improving the drought resistance and the nitrogen utilization efficiency of the rice. Background Among the near half of the global population's grain needs, rice (Oryza sativa l.) is one of the most important crops. In recent years, the lack of water resources caused by environmental changes has been a serious problem for all mankind, and the lack of water resources affects the absorption and utilization ability of nitrogen and the viability of crops, which results in great yield reduction of crops. In order to ensure sustainable development of agriculture, it is critical to enhance drought resistance and nitrogen utilization efficiency of rice. There is a clear interaction between drought tolerance and nitrogen utilization efficiency of crops. Studies have shown that nitrogen assimilation in rice is inhibited when dealing with environmental stresses such as drought. The C 2H2 z nc-f i nger transcription factor DST (drought and sa l t to l erance) responding to drought and salt stress of rice inhibits nitrogen assimilation under drought stress by regulating and controlling the expression of nitrate reductase OsNRT1.2, and shows an adaptive metabolic reprogramming. Such metabolic reprogramming helps to improve drought tolerance of rice, but is a challenge for breeding rice varieties with high drought tolerance and high nitrogen utilization efficiency. OsWOX13 is a plant-specific member of the WUSCHEL-related homeobox (WOX) gene family, which contains a highly conserved DNA binding motif ATTGATT in its Homeobox Domain (HD). At present, no research report on whether OsWOX13 regulates drought tolerance and nitrogen utilization efficiency of rice exists. Disclosure of Invention The invention provides application of an OsWOX13 gene in synergistically improving drought resistance and nitrogen utilization efficiency of rice. In a specific embodiment, the amino acid sequence encoded by the OsWOX13 gene is shown as SEQ ID NO. 2. In a specific embodiment, the nucleic acid sequence of the OsWOX13 gene is shown as SEQ ID NO. 1. The invention also provides a rice breeding method for synergistically improving the drought resistance and the nitrogen utilization efficiency of the rice, which comprises the step of improving the expression level of the OsWOX13 gene in the rice. In a specific embodiment, the expression level of the OsWOX13 gene is increased by transferring into an overexpression cassette of the OsWOX13 gene or introducing a mutation for increasing the in-situ expression of the OsWOX13 gene. In a specific embodiment, the OsWOX13 gene having a higher expression level is introduced into rice by hybridization to increase the expression level of the OsWOX13 gene. In a specific embodiment, the amino acid sequence encoded by the OsWOX13 gene is shown as SEQ ID NO. 2. In a specific embodiment, the nucleic acid sequence of the OsWOX13 gene is shown as SEQ ID NO. 1. The invention digs the multifunctional function of OsWOX13 in regulating and controlling the drought stress response and nitrogen utilization efficiency of rice, provides a new view for creating high-quality rice resources, and provides a molecular basis for the future collaborative cultivation of rice with drought tolerance and high nitrogen utilization efficiency. Drawings FIG. 1 shows a schematic diagram of OsWOX13 gene structure and CRISPR editing mode. The upper half of the figure shows a gene structure schematic diagram of OsWOX13, and simultaneously shows an editing mode of the sequence by using CRI SPR knockout technology. FIG. 2 identification of OsWOX13 overexpression and complementation material positive pedigree. Panel (A) is the identification of OsWOX13 overexpressing material positive families. (B) The figure shows the creation mode of OsWOX13 complementary material and the identification of positive family. FIG. 3 OsWOX13 upregulates rice response to drought stress. (A) The figures are phenotypic statistics of WT (ZH 11), osWOX13 overexpression (OE 13), osWOX mutant, and complementary material (Com13#1) before drought treatment and after rehydration. Panels (B-E) are water loss and survival statistics after drought treatment of the above materials. FIG. 4 representative plant photographs of WT, osWOX13 overexpression (OE 13) and OsWOX mutant materials under different nitrogenous fertilizer supply conditions (0, 100 and 200kg ha -1). FIG. 5 photographs of rice ears of representative plants after drought treatment of WT, osWOX13 overexpression (OE 13) and OsWOX mutant materials. FIG. 6 tillering statistics for WT, osWOX13 overexpression (OE 13) and OsWOX mutant mater