CN-119423018-B - Method for mixed feeding of two aphid parasitic wasps

CN119423018BCN 119423018 BCN119423018 BCN 119423018BCN-119423018-B

Abstract

The invention belongs to the technical field of natural enemy insect artificial propagation and agricultural pest biological control, and discloses a method for mixed feeding of two aphid parasitic bees, which is mainly technically characterized by comprising the following steps: the method comprises the steps of breeding host plants, inoculating and breeding aphids, obtaining and inoculating two aphid parasitic wasp species, collecting parasitic wasps, removing impurities and separating the two parasitic wasps. According to the method for mixed feeding of two aphid parasitic bees, two parasitic natural enemies of the aphidius gifuensis and the aphidius gossypii are respectively inoculated in different time periods, so that the parasitic efficiency of a single bee is improved, the interspecies competition among adult bees is avoided, and meanwhile, the two parasitic bees are collected, and manpower and material resources are saved.

Inventors

FANG MEIJUAN
ZHOU HONGYAN
WANG YUBO
HE XIAOQING
LIU DONG
SONG KAI
ZHANG HENG
JIA JINGLIANG
WANG ZHIJIE
SHI XUEQIAN

Assignees

河北省农林科学院旱作农业研究所

Dates

Publication Date: 20260508
Application Date: 20241113

Claims (2)

1. The method for mixed feeding of two aphid parasitic wasps is characterized by comprising the following steps: First step propagation of host plants Filling nutrition soil into a small-sized nutrition pot to a height of 4-6 cm, and then sowing eggplant seeds for seedling, transplanting the eggplant seedlings into a medium-sized seedling raising pot when the eggplant seedlings are 4-6 cm high, and spraying water periodically until the eggplant seedlings grow to 6-8 leaves for later use; second step of aphid inoculation and propagation Inoculating aphids which are green peach aphids, cutting the leaf blades of the eggplant seedling with the aphids into squares with the side length of 2cm, putting the back of each square on new eggplant seedling plants with 6-8 pieces downwards, putting the single plant in an insect-raising cage with 120 meshes, wherein the temperature in artificial climate is 20-28 ℃ and the humidity is 50-80%, removing old leaves of the squares after the aphids are transferred to new eggplant seedling plants, and inoculating aphid cocoons when the number of the aphids on the new eggplant seedling plants reaches 800-1000 per plant after 4-5 days; Third step, obtaining and inoculating two aphid parasitic wasp species Collecting pupa of two parasitic bees of Aphidius gifuensis and Aphidius gossypii on eggplant seedling, selecting individual with big head and bright color, placing in a pest culturing tube, arranging a round hole on the cover of the pest culturing tube, sticking the round hole with 100 meshes, placing the pest culturing tube in a constant temperature box with the temperature of 24-26 ℃ and the humidity of 60-80%, observing the emergence condition of the pupa every day, placing the adult Aphidius gifuensis in 24 hours in a pest culturing cage, inoculating the adult Aphidius gifuensis with the inoculation density of 40-50 heads/strain, inoculating the adult Aphidius gifuensis in the pest culturing cage again with the inoculation density of 40-50 heads/strain within 24 hours after inoculating, and placing in a artificial climate chamber with the temperature of 25-28 ℃ and the humidity of 50-80 ℃ after inoculating; fourth step parasitic wasp collection When only white or light brown spherical parasitic pupa of aphidius gifuensis in the insect raising cage appears on the eggplant seedling leaves, starting to record time, waiting for 2-3 days, collecting a large number of black pupa of aphidius gifuensis, picking off the leaves carrying two parasitic pupa, and flushing with clear water of a pressurized water gun to obtain two parasitic pupa; Fifth step of removing impurities Repeatedly washing and separating the two kinds of parasitic wasp pupa, removing heavy small soil and sand, air drying the two kinds of parasitic wasp pupa on gauze, and sieving the two kinds of parasitic wasp pupa with 40 mesh sieve after 1-2 days to remove fine impurities, wherein both parasitic wasp pupa will not fall down; Sixth step, two parasitic wasps are separated The pupas of the two parasitic bees are different in size, and the pupas of the aphidius gifuensis are larger than those of the Aphis gossypii; the method comprises the steps of separating two types of bee pupas through screens with different meshes, placing a 40-mesh screen at the lower layer, ensuring that the screened bee pupas cannot fall down, placing a 25-mesh screen and a 30-mesh screen at the middle layer, separating smaller aphidius gifuensis and cotton aphidius gifuensis pupa, wherein the upper layer is a 20-mesh screen for separating larger aphidius gifuensis, sieving sequentially from top to bottom, firstly placing the two types of bee pupas on the upper layer screen, repeatedly sieving for 2 times, leaving the larger aphidius gifuensis pupa by the upper layer screen, dropping into the smaller aphidius gifuensis and cotton aphidius gifuensis pupa of a 25-mesh screen and a 30-mesh screen, sieving the two types of screens at the middle layer from high to low for 2-3 times, leaving the middle layer screen as aphidius gifuensis pupa, leaking to the lower layer as cotton aphidius gifuensis pupa, separating the two types of parasitic bee pupas, and respectively collecting the two types of bee pupas for standby.
2. The method for mixed feeding of two aphid parasitic wasps according to claim 1, wherein the small-size nutrition pot is in a barrel shape with the diameter of 8cm and the height of 7.5 cm, the medium-size seedling raising pot is in a barrel shape with the diameter of 12 cm and the height of 7.5 cm, the length of the insect raising cage is 50 cm, the width of the insect raising cage is 50 cm, the height of the insect raising cage is 50 cm, the diameter of the insect raising pipe is 2.5 cm, the height of the insect raising pipe is 8cm, the diameter of a round hole of the insect raising pipe cover is 1.5 cm, the rated flow of the pressurizing water gun is 2.4m 3 /h, and the power is 0.75kW.

Description

Method for mixed feeding of two aphid parasitic wasps Technical Field The invention belongs to the technical field of natural enemy insect artificial propagation and agricultural pest biological control, and particularly relates to a method for mixed feeding of two aphid parasitic bees. Background Aphids can not only harm crops but also spread viruses, greatly influencing the yield of the crops. The aphidius gifuensis and the aphidius gossypii are parasitic natural enemies of various aphids such as cotton aphids, peach aphids and the like, and are applied to control of greenhouse vegetable cotton aphids in some countries. At present, most natural enemy insects are independently raised, and parasitic bees prefer to parasitic 2-3 years old aphids, so that a large number of 1-year old aphids are not parasitic, and in addition, the aphids are continuously bred into offspring, so that a single natural enemy is inoculated, a large number of low-year old aphids are easily caused to be not parasitic, thereby causing resource waste and low breeding efficiency. Disclosure of Invention The technical problem to be solved by the invention is to provide a method for mixed feeding of two aphid parasitic wasps, which is short in time and good in effect. In order to solve the technical problems, the technical scheme adopted by the invention comprises the following steps: First step propagation of host plants Filling nutrition soil into a small-sized nutrition pot to a height of 4-6 cm, and then sowing eggplant seeds for seedling, transplanting the eggplant seedlings into a medium-sized seedling raising pot when the eggplant seedlings are 4-6 cm high, and spraying water periodically until the eggplant seedlings grow to 6-8 leaves for later use; second step of aphid inoculation and propagation Inoculating aphids which are green peach aphids, cutting the leaf blades of the eggplant seedling with the aphids into squares with the side length of 2cm, putting the back of each square on new eggplant seedling plants with 6-8 pieces downwards, putting the single plant in an insect-raising cage with 120 meshes, wherein the temperature in artificial climate is 20-28 ℃ and the humidity is 50-80%, removing old leaves of the squares after the aphids are transferred to new eggplant seedling plants, and inoculating aphid cocoons when the number of the aphids on the new eggplant seedling plants reaches 800-1000 per plant after 4-5 days; Third step, obtaining and inoculating two aphid parasitic wasp species Collecting pupa of two parasitic bees of Aphidius gifuensis and Aphidius gossypii on eggplant seedling, selecting individual with big head and bright color, placing in a pest culturing tube, arranging a round hole on the cover of the pest culturing tube, sticking the round hole with 100 meshes, placing the pest culturing tube in a constant temperature box with the temperature of 24-26 ℃ and the humidity of 60-80%, observing the emergence condition of the pupa every day, placing the adult Aphidius gifuensis in 24 hours in a pest culturing cage, inoculating the adult Aphidius gifuensis with the inoculation density of 40-50 heads/strain, inoculating the adult Aphidius gifuensis in the pest culturing cage again with the inoculation density of 40-50 heads/strain within 24 hours after inoculating, and placing in a artificial climate chamber with the temperature of 25-28 ℃ and the humidity of 50-80 ℃ after inoculating; fourth step parasitic wasp collection When only white or light brown spherical parasitic pupa of aphidius gifuensis in the insect raising cage appears on the eggplant seedling leaves, starting to record time, waiting for 2-3 days, collecting a large number of black pupa of aphidius gifuensis, picking off the leaves carrying two parasitic pupa, and flushing with clear water of a pressurized water gun to obtain two parasitic pupa; Fifth step of removing impurities Repeatedly washing and separating the two kinds of parasitic wasp pupa, removing heavy small soil and sand, air drying the two kinds of parasitic wasp pupa on gauze, and sieving the two kinds of parasitic wasp pupa with 40 mesh sieve after 1-2 days to remove fine impurities, wherein both parasitic wasp pupa will not fall down; Sixth step, two parasitic wasps are separated The pupas of the two parasitic bees are different in size, and the pupas of the aphidius gifuensis are larger than those of the Aphis gossypii; the method comprises the steps of separating two types of bee pupas through screens with different meshes, placing a 40-mesh screen at the lower layer, ensuring that the screened bee pupas cannot fall down, placing a 25-mesh screen and a 30-mesh screen at the middle layer, separating smaller aphidius gifuensis and cotton aphidius gifuensis pupa, wherein the upper layer is a 20-mesh screen for separating larger aphidius gifuensis, sieving sequentially from top to bottom, firstly placing the two types of bee pupas on the upper layer screen, repeatedly sieving for 2 t