CN-119462873-B - Rice SDV1 gene, encoding protein and application thereof

Abstract

The rice dwarf mutant strain height of most reports at present changes drastically, and the yield is seriously affected, so that the rice dwarf mutant strain is difficult to be practically utilized in breeding. At present, the gene for fine tuning is extremely lack of the gene which can dwarf plant height in small amplitude under sd1 background. The application relates to a gene SDV1 for regulating plant height. The nucleotide sequence of the SDV1 gene can be, for example, a mutant, allele or derivative by adding, substituting, inserting or deleting one or more nucleotides in the nucleotide sequence shown in SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO. 4. According to the application, through phenotype analysis, genetic analysis, gene cloning and expression analysis of two dwarf mutants, the functions of the dwarf mutants in the regulation and control of the plant height development of rice are clarified, and a favorable gene resource is provided for rice lodging-resistant breeding.

Inventors

• LI SHUANGCHENG
• ZHANG XU
• ZOU TING
• LI PING
• LIANG YUEYANG
• WANG SHIQUAN
• DENG QIMING
• ZHU JUN
• JIN JINGHUA

Assignees

• 四川农业大学

Dates

Publication Date

20260505

Application Date

20241030

Priority Date

20240226

Claims (5)

1. 1. The application of down regulating the expression level of SDV1 gene in rice or down regulating the expression level of protein coded by SDV1 gene in rice or reducing the activity of protein coded by SDV1 gene in rice in increasing the thickness of stem wall between basal nodes of rice is characterized in that the protein SDV1 sequence is shown as the amino acid sequence of SEQ ID NO. 1.
2. 2. The application of down regulating the expression level of SDV1 gene in rice or down regulating the expression level of protein coded by SDV1 gene in rice or reducing the activity of protein coded by SDV1 gene in rice in shortening the length of stem node of rice is characterized in that the protein SDV1 sequence is the amino acid sequence shown in SEQ ID NO. 1.
3. 3. A method for improving the lodging resistance of rice comprising the steps of: down-regulating the expression quantity of SDV1 gene in rice, Or down-regulating the expression level of the protein encoded by the SDV1 gene in rice, Or decreasing the activity of a protein encoded by the SDV1 gene in rice; Wherein the nucleotide sequence of the SDV1 gene is shown as SEQ ID NO.2 or SEQ ID NO. 3.
4. 4. The method of claim 3, wherein the method of down-regulating the expression level of the SDV1 gene in rice, down-regulating the expression level of the protein encoded by the SDV1 gene in rice, or reducing the activity of the protein encoded by the SDV1 gene in rice comprises: Gene knockout, RNA interference, physicochemical mutagenesis and natural mutation are used for generating mutation in an SDV1 gene or a promoter region, so that the expression level of the SDV1 is reduced, the abundance of the SDV1 protein is reduced or the activity of the SDV1 protein is reduced.
5. 5. The method of claim 3, wherein the method of down-regulating the expression level of the SDV1 gene in rice, down-regulating the expression level of the protein encoded by the SDV1 gene in rice, or reducing the activity of the protein encoded by the SDV1 gene in rice comprises: transferring SDV1 gene or expression construct/vector containing SDV1 gene fragment into rice, targeting construction of down-regulating or inactivating mutant of SDV 1; And performing physicochemical mutagenesis on the rice, and directionally screening mutants of the SDV1 gene.

Description

Rice SDV1 gene, encoding protein and application thereof Technical Field The invention relates to the field of plant molecular breeding, in particular to a rice SDV1 gene, a coding protein and application thereof. Background Lodging is the phenomenon that a vertically grown crop is askew and even the whole plant falls to the ground. Lodging can not only lead to reduced crop yield and quality, but harvesting can also become exceptionally difficult. For example, when wheat and rice suffer severe lodging, the yield may be reduced even by more than half. As lodging usually occurs in the middle and later stages of crop growth, namely the most critical stage of crop inoculation, the loss is more serious when cereal crops such as rice, wheat and the like lodge after node extraction, wherein the plant height is one of the main factors that the crops are affected to cause lodging, and therefore, the rice plant height is closely related to the high yield/stable yield of the crops. The reason for lodging of rice can be summarized as follows: firstly, the variety factors, and the lodging resistance of different varieties is quite different. The lodging-resistant high-quality rice variety generally has the characteristics of short plant height, thick stalks, firm leaves and the like; Secondly, the fertility factor is that excessive application of nitrogen fertilizer, especially in the late growth stage of rice, can lead to the reduction of the cellulose content of stem basal cells, the thinning of cell walls and the loose tissue structure, so that lodging is easy to occur; In addition, water layer management is also an important factor, and the water layer management can keep too high water level for a long time, and has poor soil permeability, poor root system development, easy poisoning and weak root system strength, so that the rice grows too high, the stems are soft and the lodging resistance is reduced; in addition, attack by diseases and insect pests can cause the rice to grow weak and lodge, such as banded sclerotial blight and the like; finally, cultivation management is also an important factor, such as close planting, shallow cultivation and the like, which can lead to the elongation of the basal internodes of paddy rice and the loosening of tissues and easy lodging. At present, the coded gibberellin (Gibberellins, GAs) biosynthetic enzyme of a semi-dwarf mutant gene (sd 1) in the prior art is introduced into modern cultivated rice, so that the rice has the dual advantages of close planting resistance and lodging resistance, thereby greatly improving the yield of the rice. It can be said that this is a "green revolution" starting from plant height. In the current rice breeding process, the semi-dwarf phenotype becomes one of the most important breeding targets, is the basis of high and stable yield of modern rice, and shows the importance of plant height to rice yield. Numerous genetic studies have shown that rice plant types are primarily involved in the synthesis, metabolism and signal transduction pathways and interactions of various plant hormones such as Gibberellin (GA), brassinolide (BR), solitary angle Jin Nazhi (SL) and auxin (IAA). Also disclosed in the prior art are documents related in part to plant height regulation, such as the OsNramp mutant disclosed in CN117447567a and related products and applications thereof, wherein the use of the amino acid sequence of the OsNramp mutant in plant height regulation is mentioned. However, with the change of modern cultivation conditions, such as direct seeding, light and simple cultivation, mechanized operation and the like, higher requirements are put on the plant type and lodging resistance of rice. In order to adapt to the changes, breeders propose a new idea of further reducing the plant height by a small margin on the basis of the semi-dwarf mutant gene and cultivating 'dwarf-medium dwarf' -type rice. The scheme has the advantages of 1) further improving lodging resistance, 2) adapting to direct seeding and mechanized operation, 3) improving planting density, ensuring total effective spikes and avoiding the influence of plant height reduction on yield. Recently, liu et al found a novel rice plant height regulatory gene named Shortened Basal Internodes (SBI), and developed some novel rice varieties with lodging resistance, high yield and stable yield and strong adaptability by using allelic variation, and proved that a molecular breeding strategy for culturing 'dwarf-medium-short' rice based on a semi-short mutant gene is feasible. It is apparent that the implementation of this scheme depends on the mining of gene resources that are capable of fine-tuning plant height on the basis of the semi-dwarf mutant gene. However, such fine-tuning plant height genes are currently relatively scarce. Therefore, the new rice semi-dwarfing gene resource is continuously excavated and deeply researched, the yield potential and stability of the rice can be possibly furthe