CN-119506298-B - Application of AtGT1 gene in plant root system development

CN119506298BCN 119506298 BCN119506298 BCN 119506298BCN-119506298-B

Abstract

The invention discloses an application of an AtGT1 gene in plant root system development, which is characterized in that the base sequence of the AtGT1 gene is shown in a sequence table SEQ ID NO.1, and the AtGT1 gene is used for negatively regulating plant root system development. The invention obtains the AtGT1 gene mutant arabidopsis for the first time, verifies that the application of the AtGT1 gene in the arabidopsis regulates and controls the growth and development of the lower part of the plant, enriches the theoretical research of the AtGT1 gene in the development of the plant root system, provides a theoretical basis for cultivating new varieties of developed plant root systems, has important significance for further understanding the growth mechanism of the plant root systems, and can also provide more genetic resource information for improving the plant root systems on the molecular level.

Inventors

LI JIAJIA
BAI PENGYU
WANG XIAOBO
WANG WEI
WANG GUOFAN
HU XIAOYU

Assignees

安徽农业大学

Dates

Publication Date: 20260512
Application Date: 20241114

Claims (1)

The application of the AtGT1 gene in plant root system development is characterized in that the base sequence of the AtGT1 gene is shown in a sequence table SEQ ID NO.1, the AtGT1 gene is used for negatively regulating plant root system development, and the plant is Arabidopsis thaliana.

Description

Application of AtGT1 gene in plant root system development Technical Field The invention relates to the technical field of genetic engineering, in particular to application of an AtGT1 gene in plant root system development. Background Roots play a vital role in the development of plants, mainly including fixing the plant to the soil, providing mechanical support for the plant, absorbing moisture and nutrients to sustain growth, and establishing beneficial relationships with the microbiota. The space-time structure of the root is called root system configuration (Root system architecture, RSA), which has plasticity in plant growth, and this mechanism ensures that plants can adapt to environmental changes, absorb nutrition and moisture better, and maintain photosynthesis of overground plants. With environmental changes and the advent of various biotic and abiotic stresses, good RSA can provide effective protection to plants and maintain normal growth and development of individuals. The triple-helical transcription factor family is named for its characteristic highly conserved triple-helical domain (helix-loop-helix) which binds specifically to GT elements, a light-responsive DNA element, and is therefore also referred to as the GT family. According to previous studies, the GT family is divided into five subfamilies in plants, GT-1, GT-2, GT gamma, SH4 and SIP1, and furthermore, soybean contains one subfamilies GTdelta. The GT family was found to be primarily responsible for the light response as reported earlier. In Arabidopsis, the mRNA expression level of GT-4 was significantly induced by white light. Rice GT-1 family transcription factor Osrml1 is significantly down-regulated under sustained light conditions, and transcript levels of Osrml1 also exhibit rhythmic changes as light and darkness alternate. Under red light treatment, the transcription level of the soybean GT family transcription factor GmGT-2 in the yellowing seedlings is obviously down-regulated. The GT family is found to play an important role in the growth and development of plants in different periods. The soybean GmGT-2A and GmGT-2B genes contain two triple helix domains, and the activity of the arabidopsis plants over-expressing the two genes is obviously higher than that of the wild type plants under the conditions of salt treatment, drought treatment and low temperature treatment. However, few reports have been made on the influence of GT1 family genes on plant root development, and the biological role of GT1 proteins in influencing plant root development is not clear. Disclosure of Invention The invention aims to provide the application of the AtGT1 gene in the development of plant root systems, which can regulate the growth and development efficiency of the plant root systems, enrich the theoretical research of the AtGT1 gene in the development of the plant root systems and provide a theoretical basis for cultivating new varieties of plants with developed root systems. In one aspect of the invention, the invention provides application of an AtGT1 gene in plant root system development, and according to the embodiment of the invention, the base sequence of the AtGT1 gene is shown in a sequence table SEQ ID NO.1, and the AtGT1 gene is used for negatively regulating plant root system development. Compared with the prior art, the invention has the beneficial effects that: According to the invention, the AtGT1 gene mutant arabidopsis is obtained for the first time, the application of the AtGT1 gene in the arabidopsis for regulating and controlling the growth and development of the plant underground part is verified, the theoretical research of the AtGT1 gene in the development of the plant root system is enriched, a theoretical basis is provided for cultivating new varieties of developed plant root systems, the method has an important meaning for further understanding the growth mechanism of the plant root system, and more genetic resource information can be provided for improving the plant root system at the molecular level. Drawings FIG. 1 is a three-level structure model diagram of the AtGT1 protein in example 1 of the invention; FIG. 2 is a diagram of the three-primer method of example 2 of the present invention for identifying atgt.sup.1 homozygous mutant Arabidopsis, wherein LP and RP are primers for two measurements of the T-DNA insertion site on the plant genome, BP is a primer on the T-DNA segment, and M represents a marker; FIG. 3 is a diagram showing the T-DNA insertion position of atgt mutant in example 2 of the present invention; FIG. 4 is a screen of atgt mutant Arabidopsis positive plants in example 2 of the present invention; FIG. 5 is a graph showing the nutrient growth of atgt mutant plants in example 2 of the present invention compared to that of control plants in 1/2MS medium, wherein (a) root phenotype map, (b) statistics of numbers of side roots of WT and atgt1 mutants, and (c) statistics of main root lengths of WT and