CN-119530097-B - Streptomyces luteolin and application thereof in preventing and controlling tomato leaf mold

Abstract

The invention discloses streptomyces luteus and application thereof in preventing and controlling tomato leaf mold. Streptomyces luteolin (Streptomyces luteoverticillatus) RZ0102 with a strong inhibiting effect on tomato leaf mold is screened from river mud at the junction of sunlight Fu Tonghe and the sea, and the preservation number of the strain is CGMCC NO.15219. Experiments show that the strain has genetic stability in function, the fermentation supernatant has excellent thermal stability, acid-base stability and ultraviolet radiation stability, and the strain RZ0102 and the metabolite thereof can inhibit the growth of the tomato leaf mold bacteria with high efficiency.

Inventors

• SHEN PANHUI
• FANG ZHAN
• ZHAO LIN
• SONG KAIQIANG

Assignees

• 浙江欧格纳科海洋生物科技有限公司

Dates

Publication Date

20260505

Application Date

20241230

Claims (5)

1. 1. Streptomyces luteolin (Streptomyces luteoverticillatus) RZ0102, wherein the preservation number of the Streptomyces luteolin RZ0102 is CGMCC NO.15219.
2. 2. The fermentation broth of Streptomyces luteolin RZ0102 according to claim 1.
3. 3. The preparation method of the fermentation broth of claim 2, which is characterized in that after activating streptomyces luteus RZ0102 strain, seed broth is prepared by shaking culture of a culture medium of Gao's first at a constant temperature of 28-32 ℃ for 10-15 h, and the seed broth is inoculated into the fermentation broth according to an inoculum size of 1-10% and is fermented and cultured for 16-20 h at a temperature of 28-32 ℃ to obtain the fermentation broth.
4. 4. The method for preparing a fermentation broth according to claim 3, wherein the fermentation medium comprises, by weight, 2% of soluble starch, 0.05% of KNO 3 0.1%,K 2 HPO 4 , 0.5% of glucose, 0.2% of urea, 0.2% of peptone, 0.05% of MgSO 4 ·7H 2 O, and the balance of water, and has a pH of 7.2-7.5.
5. 5. Use of streptomyces luteus RZ0102 according to claim 1 or the zymogen liquid according to claim 2 for controlling tomato leaf mold.

Description

Streptomyces luteolin and application thereof in preventing and controlling tomato leaf mold Technical Field The invention relates to streptomyces luteus and application thereof in preventing and controlling tomato leaf mold, belonging to the technical field of microorganisms. Background Streptomyces luteus (Streptomyces luteoverticillatus) is an actinomycete with high development value in Streptomyces (Streptomyces). At present, less research is conducted on streptomyces luteus. CN108192829a reports that streptomyces luteus strain HY61 can be used for preventing and controlling colletotrichum gloeosporioides, botrytis cinerea, fusarium oxysporum, gibberella wheat and curvularia zeae plant pathogen, CN112899181a reports that streptomyces luteus cctccc No. M2020262 has an inhibiting effect on peanut southern blight, peanut root rot and peanut crown rot. However, no report of preventing and controlling tomato leaf mold by using streptomyces luteus is found at present. Tomato leaf mold is a very serious foliar disease on tomatoes caused by brown mold (Cladosporium fulvum). Once the leaf mold occurs, the leaf mold can be rapidly expanded, the popularity is extremely strong, the yield and the yield of the light people are reduced, and the heavy people are out of order. At present, chemical agent control is the mainstream, but the problems of environmental pollution, agricultural product safety and the like caused by chemical agents are increasingly serious. At present, biological control is widely focused on the concept of safety, health and green, but the biological control on tomato leaf mold is mainly concentrated on bacillus, and the excavation of actinomycete resources is less. Disclosure of Invention Aiming at the problems, the invention provides the streptomyces luteus and the application thereof in preventing and controlling the tomato leaf mold, the streptomyces luteus has genetic stability in function, the fermentation supernatant has excellent thermal stability, acid-base stability and ultraviolet irradiation stability, can efficiently antagonize the pathogen of the tomato leaf mold, namely the fulvia lutea, can be developed into a microbial agent aiming at the tomato leaf mold, and realizes green and efficient prevention and control of the tomato leaf mold. Streptomyces luteolin (Streptomyces luteoverticillatus) RZ0102 with a strong inhibiting effect on tomato leaf mold is screened from river mud at the junction of sunlight Fu Tonghe and the sea, and the strain is preserved in China general microbiological culture Collection center (preservation address: china academy of sciences 3 of the West China, north Star, university of Kogyo, beijing) with a preservation number of CGMCC No.15219 in1 month 15 of 2018. Experiments show that the strain has genetic stability in function, the fermentation supernatant has excellent thermal stability, acid-base stability and ultraviolet irradiation stability, the strain RZ0102 and the metabolite thereof can inhibit the growth of the tomato leaf mold bacteria with high efficiency, and the strain has wide application prospect in the control of the tomato leaf mold. The invention also provides a fermentation broth of the streptomyces luteus (Streptomyces luteoverticillatus) RZ 0102. The preparation method of the fermentation broth is characterized in that after the strain is activated, a first Gao culture medium is adopted to perform constant-temperature shaking culture for 10-15 hours at 28-32 ℃ to prepare a seed broth, and a large amount of fermentation culture medium is inoculated into the seed broth according to the inoculation amount of 1-10% to perform fermentation culture for 16-20 hours at 28-32 ℃ to obtain the fermentation broth. The mass fermentation culture medium comprises, by weight, 2% of soluble starch, 0.05% of KNO 3 0.1％,K2HPO4, 0.5% of glucose, 0.2% of urea, 0.2% of peptone, 0.05% of MgSO 4·7H2 O, the balance of water, and the pH is 7.2-7.5. The invention also provides application of streptomyces luteus RZ0102 or fermentation broth thereof in tomato leaf mold control. The beneficial effects of the invention are as follows: 1. High antibacterial effectiveness The strain can efficiently antagonize the pathogenic brown mold of tomato leaf mold. The inhibition rate of the flat plate is up to 90.83%, the fermentation broth can effectively inhibit the occurrence and the diffusion of the tomato leaf mold, the in-vitro test of the tomato leaf blade shows that the control effect of the tomato leaf mold reaches 74.94%, the field effect test shows that the control effect of the tomato leaf mold reaches 86.85%, and the tomato leaf mold has potential in control of the tomato leaf mold. 2. Antibacterial stability The strain has genetic stability functionally, and the fermentation supernatant has excellent thermal stability, acid-base stability and ultraviolet irradiation stability. The actinomycetes-streptomyces luteus with better control effect on the tomato leaf m