CN-119570678-B - Enterobacter cloacae and application thereof in tobacco growth promotion

Abstract

The invention discloses enterobacter cloacae and application thereof in tobacco growth promotion, and belongs to the technical field of microorganisms. The Enterobacter cloacae FBH1 is preserved in China Center for Type Culture Collection (CCTCC), wherein the preservation address is China Wuhan, university of Wuhan, the preservation number is CCTCC NO: M20241838, and the preservation time is 2024, 08 and 23 days. According to the invention, the enterobacter cloacae (Enterobacter cloacae) FBH1 is obtained by first separation, and the strain FBH1 has better capabilities of phosphate and potassium dissolving, IAA secretion, siderophore production and ACC deaminase production, and can promote seed germination by obviously improving the germination rate, germination vigor, seedling length and root length of seeds, obviously promote plant growth, reduce enrichment of elements such as phosphorus, potassium and the like in soil of a tobacco planting plough layer, and improve fertilizer utilization efficiency, so that the strain FBH1 has good application prospect.

Inventors

• SUN JINGGUO
• ZHANG YUNZHENG
• SONG HONGCHANG
• XU RUI
• YIN ZHONGCHUN
• YIN GANGHUA
• RAO YONG
• SUN XUECHENG
• TIAN HUIWEN
• Yin Zhaoxin
• CHAI LIGUANG
• WU YONG
• CHEN TAO
• LIU GANG
• SUN GUANGWEI
• WANG YIWEI

Assignees

• 湖北省烟草科学研究院

Dates

Publication Date

20260512

Application Date

20241220

Claims (10)

1. 1. The enterobacter cloacae (Enterobacter cloacae) FBH1 is characterized in that the enterobacter cloacae FBH1 is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M20241838.
2. 2. A biological agent comprising the Enterobacter cloacae FBH1 of claim 1.
3. 3. The biological agent of claim 2, wherein the biological agent is a liquid bacterial agent.
4. 4. The biological agent according to claim 3, wherein the content of enterobacter cloacae FBH1 in the liquid bacterial agent is not less than 1X 10 7 cfu/mL.
5. 5. A method for preparing the biological agent according to any one of claims 2 to 4, which comprises the steps of inoculating the enterobacter cloacae FBH1 according to claim 1 into a liquid culture medium for culturing, separating the obtained culture, and then re-suspending the culture with a solvent to obtain the biological agent.
6. 6. Use of the enterobacter cloacae FBH1 of claim 1, the biological agent of any one of claims 2-4 or the biological agent obtained by the preparation method of claim 5 in at least one of the following: A1 Phosphate solubilizing, A2) potassium solubilizing, A3) IAA secretion, A4) siderophore production, A5) ACC deaminase production.
7. 7. Use of the enterobacter cloacae FBH1 of claim 1, the biological agent of any one of claims 2-4 or the biological agent obtained by the preparation method of claim 5 for promoting seed germination.
8. 8. The use according to claim 7, wherein the seed is at least one of rape seed, chinese cabbage seed, rice seed, flue-cured tobacco seed.
9. 9. Use of the enterobacter cloacae FBH1 as claimed in claim 1, the biological agent as claimed in any one of claims 2 to 4 or the biological agent obtained by the preparation method as claimed in claim 5 for improving fertilizer utilization and/or plant growth promotion.
10. 10. The use according to claim 9, wherein the plant is tobacco.

Description

Enterobacter cloacae and application thereof in tobacco growth promotion Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to enterobacter cloacae and application thereof in tobacco growth promotion. Background Flue-cured tobacco is an important cash crop in China, and the total planting area, total yield and total sales of the flue-cured tobacco all stay in the front of the world. The tobacco planting history in Hubei province is long, and is one of the main tobacco producing areas of the whole country. In recent years, the soil microbial community structure imbalance caused by long-term application of chemical fertilizer in the Hubei tobacco area further causes the enrichment of nutrient elements such as nitrogen, phosphorus, potassium and the like in the soil of the tobacco area, so that the fertilizer utilization rate is low, and the tobacco yield and quality are reduced. The growth promoting bacteria can decompose insoluble phosphorus, potassium and other elements in soil, secrete plant hormone, produce siderophores and ACC deaminase, and therefore have important roles in promoting plant growth. Specifically, the phosphate-dissolving microorganisms in the growth-promoting bacteria can convert insoluble phosphorus into soluble phosphorus through acidification, chelation and exchange reactions and release the soluble phosphorus into soil for plant absorption and utilization. The organic acid and the inorganic acid generated by the potassium-decomposing bacteria in the growth-promoting bacteria can directly promote the weathering of potassium-containing minerals by reducing the pH value of the surrounding environment, so that exchangeable potassium is slowly released, the content of quick-acting environmental potassium is increased, and the growth of plants is promoted. Microorganisms in the growth-promoting bacteria can promote plant growth by directly secreting phytohormones (IAA). Microorganisms in the growth promoting bacteria can produce siderophores to form iron chelates, so that the aim of effectively absorbing iron with very low solubility in the environment to promote plant growth is fulfilled. Microorganisms in the growth-promoting bacteria produce ACC deaminase, which breaks down ACC in synthetic ethylene into alpha-butanoic acid and NH 3, reduces ethylene levels in plants, relieves stress on plants, and further promotes plant growth. The microbial agents for promoting growth in the market at present are various in variety and different in strain, but the microbial activity is influenced by factors such as temperature, humidity and soil conditions, and the Chinese smoke area is wide in area and large in natural condition difference, so that the screening of the microbial agents for promoting growth in the Hubei smoke area and the reconstruction of beneficial microbial flora in rhizosphere are key to large-scale popularization and application. However, few reports on enterobacter cloacae simultaneously have the functions of phosphate dissolving, potassium dissolving, IAA secretion, siderophore production, ACC deaminase production, seed germination promotion, plant growth promotion and the like. Disclosure of Invention The invention aims to provide enterobacter cloacae and application thereof in tobacco growth promotion. The enterobacter cloacae FBH1 provided by the invention has better capabilities of phosphate and potassium dissolving, IAA secretion, siderophore production, ACC deaminase production and seed germination promotion and plant growth promotion, and therefore has better application prospects in the aspects of improving fertilizer utilization rate and/or plant growth promotion and the like. In a first aspect, the invention provides a strain of enterobacter cloacae (Enterobacter cloacae) FBH1, wherein the preservation number of the enterobacter cloacae (Enterobacter cloacae) FBH1 is CCTCC NO: M20241838. The strain FBH1 is isolated from rhizosphere soil of flue-cured tobacco from a typical tobacco region in the area of the wild Gu Zhen east lawn in Hubei province, county. The strain FBH1 is cultured on LB solid medium plate at 28 ℃ for 1-2d to form a round shape, white color, diameter of 2-3mm, smooth surface, non-sticky texture, easy picking up, opaque bacterial colony, clear edge and fermented taste. Further, the strain FBH1 was subjected to 16S rDNA sequencing, the 16S rDNA sequences thereof were subjected to BLAST multi-sequence alignment in NCBI (https:// www.ncbi.nlm.nih.gov /), and a phylogenetic tree was constructed, and as a result, it was found that the strain FBH1 was highly homologous to Enterobacter and MK188868.1Enterobacter cloacae strainEC-3 was in the same branch, and the strain FBH1 was named Enterobacter cloacae (Enterobacter cloacae) FBH1 in combination with morphological characteristics and molecular biological identification results. In a second aspect, the invention provides a biological agent comprising the e