CN-119587693-B - Lung-targeted nanocomposite and preparation method and application thereof

CN119587693BCN 119587693 BCN119587693 BCN 119587693BCN-119587693-B

Abstract

The invention discloses a lung-targeted nanocomposite, a preparation method and application thereof, wherein the nanocomposite comprises a carbon nitride two-dimensional nanosheet and an AIE photosensitizer loaded on the surface of the carbon nitride two-dimensional nanosheet. The nanocomposite provided by the invention has excellent aggregation-induced emission property and active oxygen generation capability, can exert the diagnostic effect of a photosensitizer, can generate a better tumor elimination effect, and can synchronously realize the diagnosis and treatment of lung cancer. In addition, the nanocomposite has the advantages of being suitable in size, capable of enhancing the retention of medicines in the lung, prolonging the treatment time, reducing the administration times, capable of being administered through air pipe spraying, quick in acting, high in local medicine concentration, small in dosage, convenient to use, small in systemic adverse reaction, easy to monitor and adjust dosage and the like.

Inventors

WANG YUANWEI
TAN HUI
WANG DONG
MA XIAOPENG
TANG BENZHONG

Assignees

深圳市儿童医院

Dates

Publication Date: 20260505
Application Date: 20240926

Claims (5)

1. The nanocomposite for realizing lung targeting by means of tracheal spray administration is characterized by comprising carbon nitride two-dimensional nano-sheets and AIE photosensitizer loaded on the surfaces of the carbon nitride two-dimensional nano-sheets; the mass ratio of the carbon nitride two-dimensional nano-sheet to the AIE photosensitizer is 1-10:1; the carbon nitride two-dimensional nano sheet is proton functionalized graphite phase carbon nitride; The AIE photosensitizer is loaded on the surface of the carbon nitride two-dimensional nano sheet through electrostatic force; The planar size of the carbon nitride two-dimensional nano sheet is 0.5-2 mu m; the AIE photosensitizer has a structure selected from the group consisting of 、、、 And One of them.
2. A method of preparing the nanocomposite of claim 1, comprising: and dissolving the carbon nitride two-dimensional nano-sheet and the AIE photosensitizer in the mixed solution of the organic phase and the water phase, and stirring for reaction to obtain the nanocomposite.
3. The method of preparing nanocomposite according to claim 2, wherein the stirring reaction is performed at a temperature of 10-35 ℃ for a time of 12-24 hours.
4. The method of claim 3, further comprising washing with water and filtering after the stirring reaction.
5. Use of the nanocomposite according to claim 1 for the preparation of a medicament for the treatment and diagnosis of lung cancer.

Description

Lung-targeted nanocomposite and preparation method and application thereof Technical Field The invention relates to the technical field of biology, in particular to a lung targeting nanocomposite and a preparation method and application thereof. Background The metastatic, diffuse and lethal properties of lung cancer give great significance to lung cancer research. However, the traditional treatment methods, such as chemotherapy and radiotherapy, are difficult to enrich the lung specifically, have great side effects, resulting in poor treatment effect, the traditional imaging methods, such as CT and MRI, have low resolution and poor precision, and especially cannot accurately identify fine early cancerous tissues, resulting in delayed illness state, and the traditional diagnosis and treatment methods cannot be realized synchronously, thus greatly limiting the diagnosis and treatment efficiency of lung cancer. Accordingly, there is a need for improvement and development in the art. Disclosure of Invention In view of the shortcomings of the prior art, the invention aims to provide a lung targeting nanocomposite, a preparation method and application thereof, and aims to solve the problem that the existing lung targeting drug cannot realize diagnosis and treatment synchronously. The technical scheme of the invention is as follows: in a first aspect of the invention, a lung-targeted nanocomposite is provided comprising carbon nitride two-dimensional nanoplatelets and an AIE photosensitizer supported on the surface of the carbon nitride two-dimensional nanoplatelets. Optionally, the mass ratio of the carbon nitride two-dimensional nano-sheet to the AIE photosensitizer is 1-10:1. Optionally, the planar dimension of the carbon nitride two-dimensional nano-sheet is 0.5-2 μm. Alternatively, the AIE photosensitizer has a structure selected from the group consisting ofOne of them. Optionally, the carbon nitride two-dimensional nanoplatelets are proton functionalized graphitic carbon nitrides. Optionally, the AIE photosensitizer is supported on the carbon nitride two-dimensional nanoplatelet surface by electrostatic force. In a second aspect of the present invention, there is provided a method of preparing a nanocomposite as described in the first aspect, comprising: and dissolving the carbon nitride two-dimensional nano-sheet and the AIE photosensitizer in the mixed solution of the organic phase and the water phase, and stirring for reaction to obtain the nanocomposite. Optionally, the temperature of the stirring reaction is 10-35 ℃ and the time is 12-24h. Optionally, after the stirring reaction, the method further comprises water washing and filtering. In a third aspect of the invention, there is provided the use of a nanocomposite as described in the first aspect for the manufacture of a medicament for the treatment and diagnosis of lung cancer. The lung-targeted nanocomposite and the preparation method and application thereof have the beneficial effects that the lung-targeted nanocomposite comprises a carbon nitride two-dimensional nanosheet and an AIE photosensitizer loaded on the surface of the carbon nitride two-dimensional nanosheet. The nanocomposite provided by the invention has excellent aggregation-induced emission property and active oxygen generation capability, can exert the diagnostic effect of a photosensitizer, can generate a better tumor elimination effect, and can synchronously realize the diagnosis and treatment of lung cancer. In addition, the nanocomposite has the advantages of being suitable in size, capable of enhancing the retention of medicines in the lung, prolonging the treatment time, reducing the administration times, capable of being administered through air pipe spraying, quick in acting, high in local medicine concentration, small in dosage, convenient to use, small in systemic adverse reaction, easy to monitor and adjust dosage and the like. Drawings FIG. 1 is a scanning electron microscope image of a g-C 3N4+ nm sheet in example 1 of the present invention. FIG. 2 is a graph showing the change in fluorescence intensity of C 3N4 @ TDP at different ratios in example 1 of the present invention. FIG. 3 is a graph showing the results of the test for the active oxygen generating capacity of C 3N4, TDP and C 3N4 @TDP in example 1 of the present invention. FIG. 4 is a graph of the results of the gassing power test of C 3N4, TDP and C 3N4 @ TDP in example 1 of the present invention. FIG. 5 is a graph showing the results of cytotoxicity and phototoxicity tests of C 3N4, TDP, and C 3N4 @ TDP in example 2 of the present invention. FIG. 6 is a graph showing the results of a pulmonary retention experiment of TDP and C 3N4 @TDP in example 3 of the present invention. FIG. 7 is a graph showing the results of experiments on lung cancer treatment of mice with C 3N4 @TDP in example 4 of the present invention. Detailed Description The invention provides a lung targeting nanocomposite and a preparation metho