CN-119592450-B - Composite microecological preparation for improving black matrix of aquaculture pond and application thereof

CN119592450BCN 119592450 BCN119592450 BCN 119592450BCN-119592450-B

Abstract

The invention discloses a compound microecological preparation for improving black matrix of an aquaculture pond and application thereof. The composite microecological preparation contains large listeria GBW-PM6, bacillus subtilis S2, bacillus cereus J19, bacillus amyloliquefaciens HFJ-7, a spore germination activator, zeolite powder, attapulgite and red steady state powder. The composite microecological preparation is prepared into particles with the diameter of 2-3 mm by low-temperature rolling and fluidized bed drying, and can block the metabolic pathway of sulfur-reducing bacteria at the bottom to generate hydrogen sulfide (H 2 S) by utilizing sulfur-containing substances after throwing the culture water body into the bottom of the pond, so that the generation of hydrogen sulfide is reduced, the degradation and conversion of residual organic matters at the bottom of the pond in the culture process are promoted, the problem of blackening and stinking of substrate sludge caused by hydrogen sulfide pollution is solved, the self-cleaning capability of the bottom of the pond and the water body is improved, the ecological culture is promoted, obvious economic benefits can be brought, and the composite microecological preparation has wide market application prospect.

Inventors

ZHANG HONGLING
LIANG JINGJING
LIU BAOTONG
MENG FANKUI
LIU JIE
WANG QIAOXIN
GUO YANLIN
ZHANG ZONGGUO
Zhuang Xuechang

Assignees

青岛尚德生物技术有限公司
山东邦盛达生物科技有限公司

Dates

Publication Date: 20260512
Application Date: 20241122

Claims (7)

1. The large Listeria GBW-PM6 strain is characterized in that the large Listeria strain is classified and named as large Listeria (Priestia megaterium) and is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.32334.
2. A composite microecological preparation for improving black matrix of an aquaculture pond is characterized by comprising the following components of Bacillus megaterium GBW-PM6 with the viable bacteria content of not less than 1.00 multiplied by 10 10 CFU/g and according to claim 1, bacillus subtilis with the viable bacteria content of not less than 2.00 multiplied by 10 9 CFU/g, bacillus cereus with the viable bacteria content of not less than 5.00 multiplied by 10 9 CFU/g, bacillus amyloliquefaciens with the viable bacteria content of not less than 1.50 multiplied by 10 10 CFU/g and a functional carrier, wherein Bacillus subtilis (Bacillus subtilis) with the preservation number of CGMCC No.19824 is selected as S2, bacillus cereus with the preservation number of CGMCC No.10052 is selected as J19, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the preservation number of CGMCC No. 10011 is selected as B.3862), the Bacillus amyloliquefaciens with the viable bacteria content of not less than 1.50 multiplied by 10 CFU/g and the functional carrier is selected as B.35 C.35-20, and the balance of 20% by weight of the composite microecological preparation comprises 20% of attapulgite by weight and 20% of the active microecological powder.
3. The composite microecological preparation for improving the black matrix of an aquaculture pond according to claim 2, wherein the spore germination activator comprises 60% of L-alanine and 40% of fructose, the zeolite powder is oblique zeolite powder with the particle size not smaller than 150 meshes, and the attapulgite is very small granular attapulgite with the diameter of 0.5-1.0 mm.
4. The composite microecological preparation for improving the black matrix of an aquaculture pond according to claim 2, wherein the composite microecological preparation is round particles with the diameter of 2-3 mm, the bacterial strain and the carrier are mixed, added with water, rolled by a roller, and dried at a low temperature of 50-60 ℃ by a fluidized bed.
5. Use of GBW-PM6 of listeria megaterium according to claim 1 for improving the black matrix of an aquaculture pond.
6. The use of the composite micro-ecological preparation according to claim 3 or 4 for improving the black matrix of an aquaculture pond, wherein the composite micro-ecological preparation is used in the black matrix of the pond, and is used once every mu of water surface at an earlier period of 3 days, and 200 g-300 g is thrown every mu of water surface at an interval of 5 days after one month.
7. The use of claim 6, wherein the pond black matrix has a gradually decreasing thickness of black mud and a significant decrease in floating hydrogen sulfide.

Description

Composite microecological preparation for improving black matrix of aquaculture pond and application thereof Technical Field The invention belongs to the technical field of microorganism application, and particularly relates to a compound microecological preparation for improving black matrixes of an aquaculture pond and application thereof. Background The protein content in the feed fed by most of aquatic animal breeding varieties is relatively high, wherein only about 25% of the protein is converted into fish and shrimp organisms, other proteins are discharged into water in the forms of excretion, residual baits and the like and sink into the bottom, the sulfur-containing amino acids mainly comprise methionine (also called methionine), cysteine and cystine, sulfate is produced by the desulfurization process of microorganisms, other sulfur-containing organic matters also comprise sulfanilic acid, sulfonated matters and the like, and sulfur-containing inorganic matters comprise sulfate, sulfite and the like. Under the action of Sulfate reducing bacteria (Sulfate-Reducing Bacteria, SRB), the Sulfate generates hydrogen sulfide (H 2S）.H2 S reacts with iron and manganese elements in water to generate black sulfide suspended particles/precipitates such as FeS, mnS and the like, so that the bottom of the pool is blackened, the bottom mud is blackened mainly by the Sulfate reducing bacteria, and the black color is an important sign of existence of pond H 2 S, when the concentration of water H 2 S is higher than 0.1-0.2 mg/L, dyspnea of fishes, shrimps and crabs is caused, the growth is slow, the vitality and disease resistance are weakened, and even acute poisoning death of animals is caused. In some aging ponds which are not dredging all the year round, residual bait and feces generated in the breeding process are continuously deposited, and in addition, the phenomena of thick black and odorous sediment layer, continuous release of toxic and harmful substances, excessive H 2 S in floating water on the ponds and poisoning of the cultured animals can be met. Microorganisms are the main driving force for promoting sulfur circulation metabolism, and have close coupling relation with carbon, nitrogen and phosphorus circulation processes. Therefore, the functional strain with the effect of controlling H 2 S is screened, the microecological preparation with the effect of removing sulfur is developed, and the method has wide application prospect in improving the black matrix of the aquaculture pond. Disclosure of Invention The invention aims to provide a composite microecological preparation for improving black matrix of an aquaculture pond and application thereof. The invention uses Shewanella alga XWS producing H 2 S as an indicator bacterium, and skillfully uses the principle that H 2 S and Fe 2+ form FeS black precipitate, screens out a functional strain with the effect of controlling H 2 S, and prepares a granular compound microecological preparation after compounding the screened functional strain and a functional carrier (auxiliary agent), and the granular compound microecological preparation is applied to an aquaculture pond with deep black and odorous sediment layer and easy generation of H 2 S for a small amount of times, thereby effectively reducing the thickness of the black and odorous sediment and the H 2 S content in the floating water. The technical scheme of the invention solves the problem that the effect of the strain is difficult to evaluate accurately due to the fact that H 2 S is easy to volatilize in the screening process of the hydrogen sulfide control functional strain. In order to achieve the aim of the invention, the invention is realized by adopting the following technical scheme: The invention provides a large-sized listeria GBW-PM6 which is classified and named as large-sized listeria (Priestia megaterium) and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No.32334. The invention also provides a composite microecological preparation for improving the black matrix of the aquaculture pond, which comprises the following components of the large Listeria GBW-PM6 with the viable bacteria content not lower than 1.00 multiplied by 10 10 CFU/g, the bacillus subtilis with the viable bacteria content not lower than 2.00 multiplied by 10 9 CFU/g, the bacillus cereus with the viable bacteria content not lower than 5.00 multiplied by 10 9 CFU/g, the bacillus amyloliquefaciens with the viable bacteria content not lower than 1.50 multiplied by 10 10 CFU/g and a functional carrier. Further, the Bacillus subtilis is selected from Bacillus subtilis (Bacillus subtilis) S2 with a preservation number of CGMCC No.19824, the Bacillus cereus is selected from Bacillus cereus J19 with a preservation number of CGMCC No.10052, and the Bacillus amyloliquefaciens is selected from Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) HFJ-7 with a pre