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CN-119592470-B - Dehalogenation bacillus with methylene dichloride anaerobic degradation capability and application thereof

CN119592470BCN 119592470 BCN119592470 BCN 119592470BCN-119592470-B

Abstract

The invention discloses the field of environmental pollution restoration, and in particular relates to dehalogenation bacillus with methylene dichloride anaerobic degradation capability and application thereof. The strain is dehalogenated bacillus strain DCM (Dehalobacter sp.) which has been preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) for 11 and 30 days in 2021, and has a preservation address of CGMCC No.24007 as a collection number of China academy of sciences of China, no. 3, north Star, kogyo, beijing. The novel dehalogenation bacillus strain DCM provided by the invention is separated and purified on polluted river sediment, and the strain is gram positive, rod-shaped, smooth in surface, and has a thallus length of 1-2 mu m and a thallus diameter of 0.3-0.5 mu m. The strain can completely convert 160 mu mol of dichloromethane into formate and acetate in 7 days in an anaerobic inorganic salt culture medium, and the degradation rate is up to 32 mu mol of D ‑1 , so that the strain can efficiently degrade dichloromethane pollutants and has wide application prospects in environmental sewage treatment and environmental remediation.

Inventors

  • JIN HUIJUAN
  • YAN JUN
  • YANG YI
  • LI XIUYING
  • WANG JINGJING
  • CUI YIRU

Assignees

  • 中国科学院沈阳应用生态研究所

Dates

Publication Date
20260505
Application Date
20241209

Claims (9)

  1. 1. A dehalogenation bacillus with the capability of anaerobic degradation of methylene dichloride is characterized in that the strain is dehalogenation bacillus (Dehalobacter sp.) DCM which is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms for 11 months and 30 days in 2021, and the preservation address is North Star Xiya 1 national academy of sciences of China, the preservation number is CGMCC No. 24007.
  2. 2. The dehalogenated bacillus with the capability of anaerobic degradation of dichloromethane according to claim 1, wherein the thallus is in a shape of a rod, gram positive, the length of the thallus is 1-2 μm, and the diameter is 0.3-0.5 μm.
  3. 3. The method of claim 1, wherein the strain is used to degrade methylene chloride contaminants in the environment.
  4. 4. The method of claim 3, wherein the environment is a body of water, sediment or soil.
  5. 5. The method of claim 1, wherein the strain is used to degrade methylene chloride contaminants in an environment under anaerobic conditions.
  6. 6. A formulation for degrading dichloromethane containing dehalogenated bacillus strain DCM according to claim 1.
  7. 7. A preparation for degrading a dichloromethane contaminant according to claim 6, wherein said preparation comprises a cell culture, a cell culture concentrate or a culture bacterial suspension of said strain.
  8. 8. A method for degrading dichloromethane pollutants is characterized in that the strain of claim 1 or the preparation of claim 6 is inoculated into an environmental sample to be treated, sodium sulfide with a final concentration of 0.05 mM is added into the environmental sample as an deoxidizer, and then anaerobic degradation of dichloromethane in the environmental sample is realized.
  9. 9. The method of claim 8, wherein the strain or formulation is inoculated at 0.1% (w/v).

Description

Dehalogenation bacillus with methylene dichloride anaerobic degradation capability and application thereof Technical Field The invention discloses the field of environmental pollution restoration, and in particular relates to dehalogenation bacillus with methylene dichloride anaerobic degradation capability and application thereof. Background Dichloromethane (dichloromethane, DCM) is an important volatile halogenated hydrocarbon and is widely used in industrial production, and the main fields include adhesives, pesticides, metal degreasing agents, paint removers and the like. Along with the continuous acceleration of the urban and industrial processes, the demand of methylene dichloride is increasing. The use of methylene chloride in large quantities has led to its passage through waste water, gases and solid waste into the environment, in particular into groundwater, soil and the atmosphere. The environmental concentration of methylene chloride tends to be high around industrial areas, landfill sites and chemical plants. The compound is volatile, has high water solubility and low adsorption capacity, and has high mobility in the environment. Furthermore, methylene chloride is potentially toxic to humans and the ecosystem. Short-term exposure can cause neurotoxicity, respiratory irritation, and skin damage, while long-term exposure can lead to hepatotoxicity and carcinogenic risk. Thus, the international cancer research Institute (IARC) classifies it as a possible human carcinogen (Group 2B). Dichloromethane is difficult to degrade naturally in the environment, and has environmental durability. In recent years, with the enhancement of environmental awareness and the implementation of related regulations, environmental control of methylene dichloride during production and use is increasingly paid attention to. At present, methylene dichloride is listed in a first list of new pollutants (2023 edition) of key management issued by the ecological environment department of China, and also is listed in a list of toxic and harmful atmospheric pollutants and a list of toxic and harmful water pollutants, which are new pollutants needing key management. The traditional physical and chemical restoration technology has high cost and is easy to produce secondary pollution. In contrast, bioremediation technology utilizes microorganisms to convert methylene chloride into low-toxicity or non-toxic substances, which is a green, economical and efficient solution. The methylene chloride aerobic degradation bacteria reported in succession so far mainly come from the genus Hyphomicrobium、Albibacter、Ancylobacter、Bacillus、Gottschalkia、Lysinobacillus、Paracoccus、Methylophilus、Methylopila、Methylobacterium and other microorganism classifications. However, even in a micro-aerobic and anoxic environment, a large amount of dichloromethane contaminants are distributed, but the anaerobic microorganism population capable of metabolizing dichloromethane is very small. For example, dehalobacteriumformicoaceticum DMC strain reported in 1996 is currently the only pure culture for anaerobic degradation of methylene chloride. As early as 2012, some culture solutions highly enriched with Dehalobacter (dehalogenated bacillus) microorganisms were considered to be capable of anaerobically metabolizing methylene chloride, but pure cultures of this dehalogenated bacillus were not obtained yet limited by the isolation technique. The method directly causes the problems of low application efficiency, insufficient adaptability, limited technical development, increased ecological risk, excessive dependence on other repair methods and the like of the methylene dichloride. Therefore, obtaining a biological material capable of anaerobically degrading dichloromethane, especially a novel dehalogenation bacillus, is a great breakthrough in the field of environmental bioremediation. Disclosure of Invention In view of the above, the present invention aims to provide a dehalogenation bacillus having the capability of anaerobic degradation of methylene dichloride and application thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The dehalogenation bacillus with the capability of anaerobic degradation of methylene dichloride is a dehalogenation bacillus strain DCM (Dehalobacter sp.) which is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms for 11 and 30 days in 2021, and has a preservation address of North Star Xiya 1 and 3 China academy of sciences of Kogyo, beijing and a preservation number of CGMCC No.24007. The thallus is rod-shaped, gram positive, and has a length of 1-2 μm and a diameter of 0.3-0.5 μm. Use of said dehalogenated bacillus, said strain in degrading dichloromethane pollutant in the environment. The use of said strain to degrade methylene chloride contaminants in an environment under anaerobic conditions. The envir