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CN-119631977-B - Method for screening AD rat model and evaluating recovery condition of AD rat after drug administration

CN119631977BCN 119631977 BCN119631977 BCN 119631977BCN-119631977-B

Abstract

The invention belongs to the field of AD drug tests by utilizing rats, and in particular relates to a method for screening an AD rat model and a method for evaluating recovery condition of an AD rat after drug administration, wherein the screening method comprises the following steps of carrying out syrup and hot water drinking tests on known normal healthy rats and AD rats to be tested; if the drinking frequency of the AD rat to be tested is obviously reduced in the S area relative to that of a normal healthy rat, and the drinking frequency of the C area is obviously increased, the AD rat to be tested is the AD rat, the method for evaluating the recovery condition comprises the following steps of carrying out syrup and hot water drinking tests on the AD rat which is continuously dosed for a plurality of days, if the drinking frequency of the AD rat in the S area is gradually increased along with the increase of the date, and the drinking frequency of the AD rat in the C area is gradually reduced, the recovery of the AD rat after the dosing is indicated, the method can simply, rapidly and painlessly screen out qualified AD rat, evaluate the recovery condition of the AD rat after the dosing by adopting a low-cost method, and effectively reduce the research and development cost and time of AD special drugs.

Inventors

  • SU XINXIANG
  • Pu Junjun
  • Liao Jinxia
  • WU ZHEN
  • ZHOU YANGFAN

Assignees

  • 桂林医学院

Dates

Publication Date
20260512
Application Date
20241209

Claims (6)

  1. 1. A method of screening a rat model for AD comprising the steps of: step 1, recording and storing ultrasonic waves emitted by a physical strength-enhancing mouse when drinking syrup; step 2, arranging a sugar water area S and a hot water area C on two sides of a test field, and playing the ultrasonic waves on one side of the S area; Step 3, establishing a standard value by using a known normal healthy mouse, putting the normal healthy mouse into the middle part of a test field, and respectively recording the drinking times of the normal healthy mouse in two areas within a specified time period T1, wherein in order to eliminate accidental errors, a plurality of repeated tests are required to be carried out so as to obtain the credible drinking times of the normal healthy mouse in the two areas; step 4, throwing the AD rat to be tested into the middle part of the test field, and respectively recording the drinking times of the AD rat to be tested in two areas within a specified time length T1; And 5, comparing the data recorded in the step 4 with the data recorded in the step 3, and if the drinking frequency of the step 4 is obviously reduced relative to the drinking frequency of the S area in the step 3 and the drinking frequency of the step 4 is obviously increased relative to the drinking frequency of the C area in the step 3, determining that the AD rat to be detected is an AD rat.
  2. 2. The method of screening a rat model for AD according to claim 1, wherein the prescribed time period T1 is not less than 3 minutes.
  3. 3. The method of screening a rat model for AD according to claim 1, wherein in step 3, the repeated test is not less than 8 times.
  4. 4. A method for evaluating recovery condition of AD mice after administration is characterized by comprising the following steps, Step 1, recording and storing ultrasonic waves emitted by a physical strength-enhancing mouse when drinking syrup; step 2, arranging a sugar water area S and a hot water area C on two sides of a test field, and playing the ultrasonic wave on one side S; Step 3, continuously taking the drug of the AD mice for a plurality of days, and sequentially carrying out a plurality of tests during the drug taking period, wherein the tests are that the drug-taking AD mice are put into the middle part of a test field, the drinking times of the drug-taking AD mice in two areas are respectively recorded within a specified time period T2, And 4, comparing the times of drinking water in the two areas recorded in the step 3, if the times of drinking water in the S area gradually rise along with the increase of the date and the times of drinking water in the C area gradually decline, the AD mice are recovered after taking the medicine, and the more obvious rising or declining trend is indicated, so that the more effective medicine is indicated.
  5. 5. The method of evaluating recovery from AD murine use according to claim 4, wherein the prescribed time period T2 is not less than 3 minutes.
  6. 6. The method of claim 4, wherein the administering is continued for a period of not less than 14 days and the number of trials is not less than 4.

Description

Method for screening AD rat model and evaluating recovery condition of AD rat after drug administration Technical Field The invention belongs to the field of AD drug tests by utilizing rats, and particularly relates to a method for screening an AD rat model and evaluating recovery condition of an AD rat after drug administration. Background The evaluation of AD mice includes the identification of AD mice and the evaluation of their extent of recovery. The identification of the AD mice has important significance on the model selection and the behavioral expression of the AD, is helpful for further understanding the pathogenic mechanism of the AD, and the evaluation of the recovery degree is favorable for reflecting the healing effect of the drug, and has important effect on the research and development of new AD specific drugs with better effect. The current common evaluation systems for AD rat models include Morris water maze, MRI technology and detection of pathological marker expression. Morris water maze is used for researching learning and memory ability damage of experimental animals, MRI technology is used for determining AD lesion sites and pathogenesis of AD lesion sites, and detection of cellular molecular level is often used for researching new drug development targets and early screening of diseases due to high specificity and multiple types, and is the key point of the current research. However, they have the disadvantages of large traumatism to rats, high requirements on laboratory staff level, high cost, etc. The Morris water maze system for evaluating the recovery condition of the AD mice is high in standard, and the experimental data are more extensive, so that the data processing capability requirement of experimenters is high. The requirements on laboratory conditions are also relatively high, and the laboratory environment is generally required to keep consistent, including temperature, sound, even time, etc. Require experiment tracking monitoring systems, etc. Meanwhile, the variety, constitution and even mood of the experimental animal can influence the swimming ability of the mice to influence experimental results and have larger errors. Disclosure of Invention The invention aims at providing a method for screening an AD rat model so as to realize efficient, convenient, low-cost and harmless distinction of an AD rat from a normal rat. The technical scheme for achieving the purpose comprises the following steps. A method for screening AD rat model comprises (1) recording and storing ultrasonic wave emitted by a physical strength rat when drinking sugar water; step 2, arranging a sugar water area S and a hot water area C on two sides of a test field, and playing the ultrasonic waves on one side of the S area; Step 3, establishing a standard value by using a known normal healthy mouse, putting the normal healthy mouse into the middle part of a test field, and respectively recording the drinking times of the normal healthy mouse in two areas within a specified time period T1, wherein in order to eliminate accidental errors, a plurality of repeated tests are required to be carried out so as to obtain the credible drinking times of the normal healthy mouse in the two areas; step 4, throwing the AD rat to be tested into the middle part of the test field, and respectively recording the drinking times of the AD rat to be tested in two areas within a specified time length T1; And 5, comparing the data recorded in the step 4 with the data recorded in the step 3, and if the drinking frequency of the step 4 is obviously reduced relative to the drinking frequency of the S area in the step 3 and the drinking frequency of the step 4 is obviously increased relative to the drinking frequency of the C area in the step 3, determining that the AD rat to be detected is an AD rat. Further, the prescribed time period T1 is not less than 3 minutes. Further, in step 3, the repetition test is not less than 8 times. Rats belong to social rodents, normal rats can transmit information through specific structures of normal brain regions and receive ultrasonic waves (USV) to change the behavior and action tendency of the rats, and through tests, normal healthy rats emit positive sounds expressed by 48-63KHZ when drinking sweet water. In order to ensure the effectiveness of positive sound, the invention records the ultrasonic wave of sugar water drunk by the physical strength mice and stores the ultrasonic wave, wherein the physical strength mice are particularly healthy and particularly strong mice screened through multiple physical strength and intelligent tests (such as endurance tests, water maze system tests and the like). The ultrasonic wave is played in a laboratory, if the brain area of the AD rat to be tested is damaged, the ultrasonic wave cannot be responded, the trend of drinking hot water or drinking sweet water of the AD rat to be tested does not have obvious bias (the normal healthy rat inevitably has o