CN-119655225-B - Space-time memory task paradigm method combining time discrimination and space memory

CN119655225BCN 119655225 BCN119655225 BCN 119655225BCN-119655225-B

Abstract

The invention discloses a space-time memory task paradigm method combining time discrimination and space memory, which comprises a running machine, an arc-shaped track and two connecting bottom plates, wherein two ends of the arc-shaped track are fixedly connected with one end of one connecting bottom plate respectively, the edges of the other ends of the two connecting bottom plates are respectively close to two ends of the running machine, so that the running machine, the arc-shaped track and the two connecting bottom plates form a closed annular track, rodents move on the track, and the arc-shaped track is provided with spaced marking points, wherein the number of the marking points is 10. The space-time memory task device disclosed by the invention is used for linking time length information with accurate position information, simulating the complex situation of combining scene memory time with space information, enhancing the comprehensiveness of data analysis, and providing a feasible experimental paradigm for the mechanism research related to scene memory and the influence on time reconstruction and dynamic interaction of brain functional connection.

Inventors

ZHENG CHENGUANG
ZHANG XUEMING
WANG XUELING
WANG YIMENG
WANG LING
YANG JIAJIA
MING DONG

Assignees

天津大学

Dates

Publication Date: 20260508
Application Date: 20250103

Claims (7)

1. A space-time memory task model method combining time discrimination and space memory comprises a running machine, an arc-shaped track and two connecting bottom plates, wherein two ends of the arc-shaped track are fixedly connected with one end of one connecting bottom plate respectively, the edges of the other ends of the two connecting bottom plates are respectively close to two ends of the running machine, so that the running machine, the arc-shaped track and the two connecting bottom plates form a closed annular track on which rodents move, the arc-shaped track is provided with spaced marking points, the number of the marking points is 10, The space-time memory task paradigm method comprises the steps of carrying out 14-day formal experiments on rodents by adopting the space-time memory task device, wherein seven days are taken as a period in the formal experiments, the rodents are controlled to eat in the first six days of each period, and the rodents are allowed to eat freely in the last day of each period, and the formal experiments are carried out 1 time per day according to the sequence of steps S1, S2 and S3; Taking one end of the running machine as a starting position, selecting a j-th mark point and a j+2-th mark point from 10 mark points in a formal experiment as rewarding points respectively, wherein j is more than or equal to 3 and less than or equal to 5, j values are random, an indication block is fixed on arc-shaped tracks outside the selected j-th mark point and the j+2-th mark points respectively, a rewarding point close to one end of the rodent leaving the running machine is taken as a near rewarding point, and a rewarding point far away from one end of the rodent leaving the running machine is taken as a far rewarding point; the direction of the rodent passing through the running machine is opposite to the movement direction of the running belt on the upper surface of the running machine; s1, free exploration stage The rodent is placed at the initial position, a medium frequency sound prompt is played to start, the rodent runs through one circle of track at any time and returns to the initial position again, the playing of the medium frequency sound prompt is finished, and no food rewards are set for the rodent in the free exploration stage; S2, rule familiarity stage Cycling the rule familiarity task for five times, wherein each rule familiarity task comprises a first long-time motion memory task and a first short-time motion memory task which are sequentially carried out; A first long-time movement memory task, namely placing food rewards at a near rewarding point, placing a rodent at a starting position, playing an intermediate frequency sound prompt to start, enabling the rodent to move on a running machine at a constant speed for 15s, playing the intermediate frequency sound prompt to end, enabling the rodent to leave the running machine and enter an arc-shaped track, and playing a low-frequency sound prompt to stop at a correct rewarding position when the rodent stops at the near rewarding point and a probe waits; The first short-time motion memory task comprises the steps of placing food rewards at a far rewarding point, placing a rodent at a starting position, playing an intermediate frequency sound prompt to start, enabling the rodent to move on a running machine at a constant speed for 6s, playing the intermediate frequency sound prompt to end, enabling the rodent to leave the running machine and enter an arc-shaped track, and playing a low-frequency sound prompt to stop at a correct rewarding position when the rodent stops at the far rewarding point and a probe waits; S3, a space-time memory task stage Performing 15 second long-time motion memory tasks and 15 second short-time motion memory tasks according to a random sequence as one session, and performing two sessions in total; A second long-time movement memory task, namely placing the rodent at a starting position, starting to play an intermediate frequency sound prompt, enabling the rodent to move on the running machine at a constant speed for 15s, ending to play the intermediate frequency sound prompt, enabling the rodent to leave the running machine and enter an arc-shaped track, and playing a low-frequency sound prompt to stop at a correct rewarding position and giving the rodent food rewarding when the rodent stops at a near rewarding point and a probe waits; A second short-time motion memory task, namely placing the rodent at a starting position, playing an intermediate frequency sound prompt to start, enabling the rodent to move at a constant speed on the running machine for 6s, playing an intermediate frequency sound prompt to end, enabling the rodent to leave the running machine and enter an arc-shaped track, and playing a low-frequency sound prompt to stop at a correct rewarding position and giving food rewards to the rodent when the rodent stops at a far rewarding point and a probe waits; in S2 and S3, during uniform movement of the rodent on the treadmill, the rodent is prevented from leaving the treadmill by the blocking plate so that the rodent can complete uniform movement for a corresponding time.
2. The spatiotemporal memory task paradigm method of claim 1 wherein the arcuate track remains horizontal and the arcuate track is in the same horizontal plane as the top surface of the treadmill.
3. The method of claim 1, wherein the space-time memory task model further comprises a barrier for blocking rodents from exiting the treadmill.
4. The method of claim 1, wherein the diet control criteria is that only rodents are allowed to obtain 20 g-25 g of diet per day.
5. The spatiotemporal memory task paradigm method of claim 1, wherein after each of steps S1, S2 and S3 the rodent holds the rodent off the spatiotemporal memory task device and rests for 5 minutes and wipes the arcuate track surface within 5 minutes of the rodent resting to remove odor marks left by the rodent.
6. The spatiotemporal memory task paradigm method of claim 1, wherein the speed of the treadmill is set to 12m/min.
7. The spatiotemporal memory task paradigm method of claim 1, wherein the rodent is held off the spatiotemporal memory task device between two sessions for 5 minutes and the arcuate track surface is wiped over within 5 minutes to remove odor marks left by the rodent.

Description

Space-time memory task paradigm method combining time discrimination and space memory Technical Field The invention belongs to the technical field of animal memory tasks, and particularly relates to a space-time memory task paradigm method combining time discrimination and space memory. Background The scenario memory is the memory of events (scenarios) experienced by an individual in person and occurring at a certain time and place by taking time and space as coordinates, is the highest-level and latest-matured memory system of a human being, is the memory system most affected by aging, and has a tendency to decrease with age. The hippocampus is an important brain structure for episodic memory, in which there is a class of neurons capable of specifically characterizing spatial information, called positional cells, capable of delivering action potentials at specific locations, referred to as the positional domains of positional cells, which are thought to form the basis of the cognitive abilities of the brain space. Also, there is a class of neurons in the hippocampus that can specifically characterize time information, called time cells, that can emit action potentials at specific moments, time domains called time cells, which are also considered to be the basis of brain time perception. The space cognition ability and the time cognition ability are important parts of the brain cognition ability, the space information and the time information are also important components of the situational memory, the space cognition and the time perception depend on neuron characterization in a task state, and the problem that the brain integrates different types of information and the like has no exact answer, so that the search of internal neural mechanisms of the space information and the time information coded in the brain has great significance. However, at present, in research on rodents, an experimental paradigm for researching the activity mechanism of information coding of time information and space information in the brain and researching the neural mechanism under the situational memory by correlating the time information and the space information still does not appear. Disclosure of Invention Aiming at the defects of the prior art, the invention aims to provide a space-time memory task device. Another object of the present invention is to provide a space-time memory task paradigm method combining time discrimination and spatial position memory, which explores the internal neural mechanism of encoding time information and spatial information in the brain under situational memory by a space-time memory task device. The aim of the invention is achieved by the following technical scheme. A space-time memory task device comprises a running machine, an arc-shaped track and two connecting bottom plates, wherein two ends of the arc-shaped track are fixedly connected with one end of one connecting bottom plate respectively, edges of the other ends of the two connecting bottom plates are close to two ends of the running machine respectively, so that the running machine, the arc-shaped track and the two connecting bottom plates form a closed annular track, rodents move on the track, and the arc-shaped track is provided with spaced marking points, wherein the number of the marking points is 10. In the technical scheme, the arc-shaped track is kept horizontal and is positioned on the same horizontal plane with the top surface of the running machine. In the technical scheme, the space-time memory task device further comprises a blocking plate for blocking rodents from leaving the running machine. A space-time memory task paradigm method combining time discrimination and space memory comprises the steps of adopting a space-time memory task device to conduct 14-day formal experiments on rodents, taking seven days as a period in the formal experiments, controlling the rodents to eat in the first six days of each period, and enabling the rodents to eat freely in the last day of each period, wherein the daily formal experiments are conducted for 1 time according to the sequence of steps S1, S2 and S3; Taking one end of the running machine as a starting position, selecting a j-th mark point and a j+2-th mark point from 10 mark points in a formal experiment as rewarding points respectively, wherein the value of j is more than or equal to 3 and less than or equal to 5,j is random, fixing an indication block on an arc track outside the selected j-th mark point and the j+2-th mark point respectively, taking a rewarding point close to one end of the rodent leaving the running machine as a near rewarding point, and taking a rewarding point far away from one end of the rodent leaving the running machine as a far rewarding point; the direction of the rodent passing through the running machine is opposite to the movement direction of the running belt on the upper surface of the running machine; s1, free exploration stage The rodent is pla