CN-119662415-B - Endophytic fungus LH001 and application thereof

Abstract

The invention discloses endophytic fungi LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from a wild rice root system, is classified and named Pseudophialophora sp.LH001, and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20241751. The endophytic fungus LH001 strain and rice are co-cultured to enable the endophytic fungus LH001 strain to be colonized at the root of rice seedlings, so that the damage of the leaves generated by rice blast bacteria can be reduced, the disease resistance of the rice seedlings to the leaf blast is enhanced, and the control effect reaches 94.05%. Therefore, the endophytic fungus LH001 has great value for popularization and application of biological control effect on rice blast in the agricultural field.

Inventors

• LIN FUCHENG
• LI LIN
• HE MENGFAN
• SU ZHENZHU
• ZHU XUEMING
• BAO JIANDONG

Assignees

• 浙江省农业科学院

Dates

Publication Date

20260512

Application Date

20240930

Claims (6)

1. 1. The endophytic fungus LH001 is separated from a wild rice root system, is classified and named Pseudophialophora sp, and is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20241751.
2. 2. The use of the endophytic fungus LH001 according to claim 1 for controlling rice blast.
3. 3. The use according to claim 2, wherein the application comprises colonizing the rice root tissue with endophytic fungus LH 001.
4. 4. The use according to claim 2, characterized in that it comprises co-cultivation with the endophytic fungus LH001 after germination of the rice seeds, so as to allow it to colonize the roots of the rice seedlings, in order to increase the resistance of the rice seedlings to Miao Shewen.
5. 5. The use according to claim 4, wherein the rice seeds germinate under 22-25 ℃ after surface disinfection, and the seeds are transferred into a 1/2MS culture medium after being exposed to the white, and simultaneously are inoculated into an endophytic fungus LH001 bacterial cake for co-cultivation.
6. 6. The use according to claim 4, wherein the co-cultivation is carried out at 22-25 ℃ until the three leaves are in one heart stage, 16 h are illuminated daily, and 8 h are dark cultivated.

Description

Endophytic fungus LH001 and application thereof Technical Field The invention relates to the technical field of plant disease control, in particular to endophytic fungi Pseudophialophora sp.LH001 and application thereof in controlling rice blast. Background The rice blast is a disease caused by Pyricularia oryzae and occurring in rice. Rice blast can occur in the whole growth period of rice, and is a pest to seedlings, leaves, ears, nodes and the like, which are respectively called seedling plague, leaf plague, ear plague and node plague, wherein the most common and most harmful are leaf plague and ear plague. The rice blast preventing and controlling method mainly selects disease-resistant varieties, cultivates high-quality seedlings, adopts fertilizer and water management technical measures, strengthens field management, realizes preventing and controlling measures, controls chemical agents and the like. The cultivation of the disease-resistant variety is one of the most economic and effective ways for preventing and treating the rice blast germ, but the physiological feculence of the rice blast germ is numerous, the mutation is strong, the adaptability is fast, so that the cultivation of the disease-resistant variety is very challenging. Chemical control is also a common method for controlling rice blast, but the method can pollute the environment and easily cause the rice blast bacteria to generate drug resistance. Therefore, the search for efficient, environment-friendly, green and safe biological control measures is a research hotspot for plant disease control. Biological control is to control plant pathogens by using living organisms or metabolic active ingredients generated by organisms, and has the advantages of environmental friendliness, difficult generation of drug resistance, small damage to natural enemies of harmful organisms, low risk to human and animal health and the like. Compared with the traditional chemical control method, the method accords with the development trend of modern agriculture, and common biological control measures are that firstly antagonistic bacteria are applied, wherein the antagonistic bacteria refer to beneficial bacteria which can produce metabolites such as antibiotics, enzymes, hormones and the like and can control pathogenic microorganisms in a bacteriostasis, competition and other modes. And secondly, applying a bioremediation agent, wherein the bioremediation agent is a microbial preparation which can decompose organic substances in soil, construct soil flora and improve soil fertility. Thirdly, applying biological bacterial fertilizer, wherein the biological bacterial fertilizer is a fertilizer rich in beneficial microorganisms, and the effect of enhancing the immunity of rice is achieved by exerting the advantage effect of microbial communities in the soil environment. Fourthly, plant-derived biological agents are applied, wherein the plant-derived biological agents refer to organic compounds extracted from plants or extracted through fermentation, and the agents can improve the immunity of rice through a plant interaction mechanism and effectively prevent and treat rice blast. A large number of beneficial microorganisms are harbored in the natural ecosystem, one of which is the endophytic fungus of the plant. Endophytic fungi are fungi that are capable of infecting, at least a portion of their life history, into healthy plant tissue without significant host disease. Is commonly in an ecological system and forms a reciprocal symbiotic relationship with host plants, thereby promoting plant growth, improving the capability of the plants for resisting biotic and abiotic stress, and the like. Therefore, the endophytic fungi can be used as potential resource bacteria such as biological antibacterial agents, biological nitrogen fixation agents, plant growth promoters and the like, and can be widely applied to crops such as grains, vegetables, fruit trees and the like. Therefore, the excavation of beneficial microorganisms with control effect on rice blast from plant endophytic fungi has important significance for the control of rice blast. Disclosure of Invention The invention aims to provide a wild rice endophytic fungus which can improve the disease resistance of rice to rice blast through symbiotic interaction with rice so as to realize the prevention and treatment of rice seedling leaf blast. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention separates a new strain of pseudobottle mould endophytic fungi from a wild rice root system, and is mainly characterized in that the diameter of a bacterial colony reaches 5.4cm after the plant grows on a PDA flat plate at 25 ℃ for 12 days, the bacterial colony is yellow green, aerial hypha is undeveloped, the hypha is creeping attached to the surface of a culture medium, the hypha is provided with a diaphragm, and conidium is elliptical or pear-shaped and has no diaph