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CN-119700706-B - Near-infrared response antibacterial nano material and preparation method and application thereof

CN119700706BCN 119700706 BCN119700706 BCN 119700706BCN-119700706-B

Abstract

The near infrared response antibacterial nano material takes CuO 2 nano particles and ICG as cores, takes phase change material PCM as a shell, has good antibacterial property, no drug resistance and stability, realizes the combined antibacterial of three modes of CDT/PDT/PTT, and has broad-spectrum antibacterial property and good biocompatibility.

Inventors

  • ZHU YINGNAN
  • DONG YUZE
  • Si Jiahang
  • ZHANG RUIKANG
  • XU TIANTIAN

Assignees

  • 郑州大学

Dates

Publication Date
20260505
Application Date
20241126

Claims (7)

  1. 1. A near infrared response antibacterial nano material is characterized in that the near infrared response antibacterial nano material takes CuO 2 nano particles and ICG as cores and takes a phase change material PCM as a shell; The preparation method of the near infrared response antibacterial nanomaterial comprises the following steps: 1) Copper chloride dihydrate and hydrogen peroxide are mixed and reacted under alkaline conditions, and ultrafiltration is carried out to obtain CuO 2 nano particles; 2) Dispersing hexadecanol and oleic acid in ethanol, carrying out ultrasonic treatment, and then carrying out vacuum drying to obtain a phase change material PCM; 3) Mixing CuO 2 nano particles, ICG and a surface modifier to obtain a solution 1, dissolving a phase change material PCM in ethanol to obtain a solution 2, mixing the solution 1 and the solution 2, performing ultrasonic treatment, immediately placing in an ice bath, and dialyzing after the ice bath to obtain a near infrared response antibacterial nano material; in the step 3), in the solution 1, the concentration of CuO 2 nano particles is 1-5mg/mL, the concentration of ICG is 0.1-5mg/mL, the surface modifier is lecithin and DSPE-mPEG, the concentration of lecithin is 1-10mg/mL, the concentration of DSPE-mPEG is 0.5-10mg/mL, in the solution 2, the concentration of phase change material PCM is 5-20mg/mL, and the volume ratio of the solution 1 to the solution 2 is 10:0.5-1; In the step 3), the ultrasonic temperature is 42-50 ℃, the ultrasonic time is 5-20min, and the time of the ice-water bath is 5-20min.
  2. 2. The near infrared response antibacterial nanomaterial of claim 1, wherein the particle size of the CuO 2 nanoparticles is 5-20nm, the particle size of the near infrared response antibacterial nanomaterial is 100-350nm, and the loading amount of the CuO 2 nanoparticles is 60% -90%.
  3. 3. The near infrared responsive antibacterial nanomaterial of claim 1, wherein the phase change material PCM has a melting point of 40-45 ℃.
  4. 4. A near infrared responsive antibacterial nanomaterial as claimed in claim 1 or 3 wherein the phase change material PCM is prepared from cetyl alcohol and oleic acid by esterification.
  5. 5. The near infrared responsive antibacterial nanomaterial of claim 4, wherein in step 1), the mixing reaction time is 30-60min and the temperature is 20-30 ℃.
  6. 6. The near infrared responsive antibacterial nanomaterial of claim 5, wherein in step 2), the mass ratio of oleic acid to cetyl alcohol is 1:2-5, and the ultrasonic treatment is performed for 5-20min.
  7. 7. Use of a near infrared responsive antibacterial nanomaterial according to any of claims 1-6 for the preparation of an antibacterial drug.

Description

Near-infrared response antibacterial nano material and preparation method and application thereof Technical Field The invention relates to the technical field of antibacterial materials, in particular to a near infrared response antibacterial nanomaterial and a preparation method and application thereof. Background Bacteria can cause various types of skin and soft tissue infections, and are one of the biggest enemies for human health today. Current therapies are mainly directed to the use of large doses of antibiotics against bacterial infections, but the rapid development and evolution of bacterial resistance makes the treatment of clinical infections difficult. In recent years, antimicrobial strategies based on chemo-and photodynamic therapy (PDT) to generate Reactive Oxygen Species (ROS) have received widespread attention. However, H 2O2 and O 2 are deficient in the skin-infected microenvironment, severely reducing their antimicrobial efficacy. The metal peroxide can react with H 2 O to produce H 2O2, and can be decomposed to produce O 2 under the heating condition. However, the application of the metal peroxide in the biomedical field is greatly limited by the characteristics of instability, easy decomposition, strong toxic and side effects and the like. Literature (Self-enhanced ROS generation by responsive co-delivery of H2O2and O2 based on a versatile composite biomaterial for hypoxia-irrelevant multimodal antibiofilm therapy,Jiayu Xiao et al, CHEMICAL ENGINEERING Journal, volume 465,1June 2023,142958), loading CuO 2 and ICG onto the surface of Polydopamine (PDA) particles, resulting in PDA/CP/ICG, where CuO 2 is only 41.5% and lower, and where PDA/CP/ICG concentrations are above 100 μg/mL, irradiation time of the near infrared light source is 10 minutes in antimicrobial applications, irradiation time is long, long near infrared irradiation may cause nanoparticle overheating, causing damage to surrounding tissues, and later application in clinic will also cause discomfort to the patient, affecting compliance with treatment. Disclosure of Invention The ICG/CuO 2 @PCM prepared by the method has good antibacterial property, no drug resistance and stability, realizes the combined antibacterial of three modes of CDT/PDT/PTT, and has broad-spectrum antibacterial property and good biocompatibility. The technical scheme of the invention is realized by adopting a near infrared response antibacterial nanomaterial, wherein the near infrared response antibacterial nanomaterial (ICG/CuO 2 @PCM) takes CuO 2 nano particles and indocyanine green (ICG) as cores and takes a phase change material PCM as a shell. Further, the particle size of the CuO 2 nm to 20nm, and the particle size of the near infrared response antibacterial nanomaterial is 100 nm to 350nm. Further, the melting point of the phase change material PCM is 40-45 ℃. Further, the phase change material PCM is prepared from cetyl alcohol and oleic acid by esterification. Further, the loading of the CuO 2 nano-particles is 60% -90%. The preparation method of the near infrared response antibacterial nanomaterial comprises the following steps: 1) Copper chloride dihydrate and hydrogen peroxide are mixed and reacted under alkaline conditions, and ultrafiltration is carried out to obtain CuO 2 nano particles; 2) Dispersing hexadecanol and oleic acid in ethanol, carrying out ultrasonic treatment, and then carrying out vacuum drying to obtain a phase change material PCM; 3) Mixing CuO 2 nano particles, ICG and a surface modifier to obtain a solution 1, dissolving a phase change material PCM in ethanol to obtain a solution 2, mixing the solution 1 and the solution 2, performing ultrasonic treatment, immediately placing into an ice bath, and dialyzing after the ice bath to obtain the near infrared response antibacterial nano material. Further, in step 1), the mixing reaction time is 30-60min, the temperature is 20-30 ℃, and the centrifugation is performed at 5000-7000rpm using a 50kD ultrafiltration tube. Further, in the step 2), the mass ratio of oleic acid to hexadecanol is 1:2-5, the ultrasonic treatment is carried out for 5-20min, and the vacuum drying temperature is 40-60 ℃. Further, in the step 3), the concentration of CuO 2 nano particles in the solution 1 is 1-5mg/mL, the concentration of ICG is 0.1-5mg/mL, the surface modifier is lecithin and DSPE-mPEG, the concentration of lecithin is 1-10mg/mL, the concentration of DSPE-mPEG is 0.5-10mg/mL, the concentration of phase change material PCM in the solution 2 is 5-20mg/mL, and the volume ratio of the solution 1 to the solution 2 is 10:0.5-1. Further, in the step 3), the ultrasonic temperature is 42-50 ℃, the ultrasonic time is 5-20min, the time of an ice-water bath is 5-20min, the molecular weight cut-off of a dialysis bag is 8-20kD, and the dialysis time is 24-48h. An application of near infrared response antibacterial nano material in preparing antibacterial medicine. The invention has the beneficial effects