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CN-119752932-B - Application of soybean GmBBX b gene in delaying senescence of leaves and plants

CN119752932BCN 119752932 BCN119752932 BCN 119752932BCN-119752932-B

Abstract

The invention discloses an application of soybean GmBBX b gene in delaying senescence of leaves and plants, wherein the nucleotide sequence of soybean GmBBX b gene is shown as SEQ ID NO.1, and the amino acid sequence of soybean GmBBX b protein is shown as SEQ ID NO. 2. The invention creates an over-expression transgenic soybean strain for GmBBX b genes, and discovers that compared with a wild type, the flowering time, leaf and plant senescence time of the GmBBX b transgenic soybean are obviously delayed, the branch number is obviously increased, and the plant height and the main stem node number are also obviously increased. Therefore, the over-expression of GmBBX b genes in soybeans is one of effective modes for changing soybean plant types and delaying soybean flowering time, leaf and plant senescence, and has important production and theoretical significance for the seed selection of soybean dominant varieties and the development of germplasm resources.

Inventors

  • XU DONGQING
  • SONG ZHAOQING
  • JIANG QING

Assignees

  • 南京农业大学

Dates

Publication Date
20260508
Application Date
20241220

Claims (16)

  1. 1. Use of any of the following for delaying senescence in soybean leaves, characterized in that said use is effected by overexpressing GmBBX b protein in soybean to delay senescence in soybean leaves: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of the soybean GmBBX b protein is as follows (A1) or (A2): (A1) An amino acid sequence as set forth in SEQ ID NO. 2; (A2) A fusion protein obtained by ligating a protein tag to the N-terminus and/or the C-terminus of (A1); the soybean GmBBX b gene encodes the soybean GmBBX b protein.
  2. 2. Use of any of the following for increasing soybean plant height, number of main stem nodes and number of branches by overexpressing GmBBX b protein in soybean to increase soybean plant height, number of main stem nodes and number of branches: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of the soybean GmBBX b protein is as follows (A1) or (A2): (A1) An amino acid sequence as set forth in SEQ ID NO. 2; (A2) A fusion protein obtained by ligating a protein tag to the N-terminus and/or the C-terminus of (A1); the soybean GmBBX b gene encodes the soybean GmBBX b protein.
  3. 3. Use of any of the following for delaying the flowering-time of soybean, characterized in that said use is effected by overexpressing GmBBX b protein in soybean to delay the flowering-time of soybean: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of the soybean GmBBX b protein is as follows (A1) or (A2): (A1) An amino acid sequence as set forth in SEQ ID NO. 2; (A2) A fusion protein obtained by ligating a protein tag to the N-terminus and/or the C-terminus of (A1); the soybean GmBBX b gene encodes the soybean GmBBX b protein.
  4. 4. Use of any of the following in soybean breeding, characterized in that said use is achieved by a soybean breeding material that achieves a soybean leaf senescence-delaying phenotype by overexpressing GmBBX b protein in soybean: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of the soybean GmBBX b protein is as follows (A1) or (A2): (A1) An amino acid sequence as set forth in SEQ ID NO. 2; (A2) A fusion protein obtained by ligating a protein tag to the N-terminus and/or the C-terminus of (A1); the soybean GmBBX b gene encodes the soybean GmBBX b protein.
  5. 5. The use according to any one of claims 1 to 4, wherein the sequence of the soybean GmBBX b gene is set forth in SEQ ID No. 1.
  6. 6. A method of delaying senescence of soybean leaves, comprising increasing the content and/or activity of GmBBX b protein as described in claim 1.
  7. 7. The method of claim 6, wherein increasing the amount and/or activity of GmBBX b protein is achieved by increasing the expression level of GmBBX b gene.
  8. 8. The method for delaying senescence of soybean leaves according to claim 7, wherein the increase of the expression level of GmBBX b gene is achieved by transferring a recombinant plant expression vector overexpressing GmBBX b into soybean, specifically by constructing GmBBX b gene fragment to JRH0641 vector by homologous recombination.
  9. 9. A method of increasing soybean plant height, number of main stem nodes and number of branches, comprising increasing the amount and/or activity of GmBBX b protein as defined in claim 1.
  10. 10. The method according to claim 9, wherein increasing the amount and/or activity of GmBBX b protein as defined in claim 1 is achieved by increasing the expression level of GmBBX b gene.
  11. 11. The method of claim 10, wherein increasing the expression level of GmBBX b gene is achieved by transferring a recombinant plant expression vector that overexpresses GmBBX b into soybean.
  12. 12. The method according to claim 11, wherein the recombinant plant expression vector is specifically constructed by constructing GmBBX b gene fragment to a JRH0641 vector by homologous recombination to obtain the recombinant plant expression vector.
  13. 13. A method for delaying the flowering time of soybeans is characterized in that, The method comprises increasing the amount and/or activity of GmBBX b protein as described in claim 1.
  14. 14. The method according to claim 13, wherein increasing the amount and/or activity of GmBBX b protein as defined in claim 1 is achieved by increasing the expression level of GmBBX b gene.
  15. 15. The method of claim 14, wherein increasing the expression level of GmBBX b gene is achieved by transferring a recombinant plant expression vector that overexpresses GmBBX b into soybean.
  16. 16. The method according to claim 15, wherein the recombinant plant expression vector is specifically constructed by constructing GmBBX b gene fragment to a JRH0641 vector by homologous recombination to obtain the recombinant plant expression vector.

Description

Application of soybean GmBBX b gene in delaying senescence of leaves and plants Technical Field The invention belongs to the field of biotechnology, and in particular relates to application of GmBBX b gene in delaying senescence of soybean leaves and plants Background Soybean is an important grain and oil crop. Leaf senescence is regulated by endogenous factors such as leaf age and plant hormone, and exogenous conditions such as illumination, temperature and pressure. The bad environments such as shading, drought, plant diseases and insect pests and the like can lead to premature senility of leaves, seriously affect photosynthetic efficiency and nutrition redistribution of crops, and further reduce yield. Therefore, the deep research of the leaf senescence regulation mechanism has important significance for optimizing the production performance of crops. The B-box (BBX) family of proteins is a class of zinc finger transcription factors. It plays an important role in the development of light-regulated processes such as seed germination, seedling photomorphogenesis, shade-avoidance, flowering, and biotic and abiotic stress responses. GmBBX13B protein contains 2B-box domains. So far, the study of soybean GmBBX b gene has not been reported. Therefore, cloning and functional research of GmBBX b gene are of great significance. Disclosure of Invention The invention aims to provide an application of soybean GmBBX b gene for delaying senescence of leaves and plants. The aim of the invention can be achieved by the following technical scheme. In a first aspect, the invention provides the use of any of the following for regulating senescence in soybean leaves and plants: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of GmBBX b protein is as follows (A1) or (A2) or (A3): (A1) A protein consisting of an amino acid sequence shown in SEQ ID NO.2 in a sequence table; (A2) And (3) the protein which is derived from the (1) and has the same function and is obtained by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown in SEQ ID NO.2 in the sequence table. (A3) A fusion protein obtained by ligating a protein tag to the N-terminus and/or C-terminus of (A1) or (A2). In a second aspect, the invention provides the use of any of the following to increase soybean plant height, main stem node number and branch number: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of GmBBX b protein is as follows (A1) or (A2) or (A3): (A1) A protein consisting of an amino acid sequence shown in SEQ ID NO.2 in a sequence table; (A2) A protein which is derived from (1) and has the same function by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown in SEQ ID NO.2 in the sequence table; (A3) A fusion protein obtained by ligating a protein tag to the N-terminus and/or C-terminus of (A1) or (A2). In a third aspect, the invention provides the use of any of the following for regulating flowering time in soybean: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing the soybean GmBBX b gene; The amino acid sequence of GmBBX b protein is as follows (A1) or (A2) or (A3): (A1) A protein consisting of an amino acid sequence shown in SEQ ID NO.2 in a sequence table; (A2) A protein which is derived from (1) and has the same function by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown in SEQ ID NO.2 in the sequence table; (A3) A fusion protein obtained by ligating a protein tag to the N-terminus and/or C-terminus of (A1) or (A2). In a fourth aspect, the invention provides the use of any one of the following in plant breeding: (1) Soybean GmBBX b gene; (2) Soybean GmBBX b protein; (3) Recombinant expression vectors, expression cassettes, transgenic cell lines or recombinant bacteria containing GmBBX b gene; The amino acid sequence of GmBBX b protein is as follows (A1) or (A2) or (A3): (A1) A protein consisting of an amino acid sequence shown in SEQ ID NO.2 in a sequence table; (A2) A protein which is derived from (1) and has the same function by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown in SEQ ID NO.2 in the sequence table; (A3) A fusion protein obtained by ligating a protein tag to the N-terminus and/or C-terminus of (A1) or (A2). In a specific embodiment, the tag refers to a polypeptide or protein that is expressed by fusion with the target protein using in vitro recombinan