Search

CN-119769496-B - Hypertension blood sample protective agent, vacuum blood collection tube for hypertension blood sample detection and application

CN119769496BCN 119769496 BCN119769496 BCN 119769496BCN-119769496-B

Abstract

The invention provides a hypertension blood sample protective agent, a vacuum blood collection tube for hypertension blood sample detection and application thereof, belonging to the technical field of medical appliances, and being characterized in that the hypertension blood sample protective agent comprises the following raw materials in parts by weight: enalapril maleate, an anticoagulant and a complexing agent; the vacuum blood collection tube for detecting the hypertension blood sample is provided with the hypertension blood sample protective agent; the application is that the vacuum blood collection tube for hypertension blood sample detection is adopted to directly store blood. The vacuum blood collection tube for hypertension blood sample detection can reduce complicated steps of reagent preparation and on-site reagent addition, thereby improving detection efficiency and accuracy of detection results.

Inventors

  • GU LIYUAN
  • XIA ZHONGBO
  • GAO HUI
  • SUN JINGNA
  • SHEN LI
  • ZHAO WEI

Assignees

  • 河北鑫乐医疗器械科技股份有限公司

Dates

Publication Date
20260512
Application Date
20241206

Claims (8)

  1. 1. The protective agent for hypertension blood sample detection is characterized by comprising, by weight, 1-1.3 parts of enalapril maleate, 15.2-18.6 parts of anticoagulant and 13.8-16.9 parts of complexing agent, wherein the complexing agent is imidazolidinyl urea and 8-hydroxyquinoline sulfate in a weight ratio of 7.5-9.2:6.3-7.7; the protective agent for hypertension blood sample detection is prefilled into a test tube for sealing, and the interior of the test tube is vacuumized.
  2. 2. The protectant for hypertensive blood sample detection of claim 1, wherein said anticoagulant is dipotassium ethylenediamine tetraacetate.
  3. 3. The protective agent for hypertension blood sample detection according to claim 1, wherein the protective agent for hypertension blood sample detection is prepared by dissolving enalapril maleate, an anticoagulant and a complexing agent in water for injection.
  4. 4. The protective agent for hypertension blood sample detection according to claim 1, wherein the protective agent for hypertension blood sample detection contains 15.2-18.6 g/100mL of ethylene diamine tetraacetic acid dipotassium, 7.5-9.2 g/100mL of imidazolidinyl urea, 6.3-7.7 g/100mL of 8-hydroxyquinoline sulfate and 1.0-1.3 g/100mL of enalapril maleate.
  5. 5. The vacuum blood collection tube for hypertension blood sample detection, which is characterized in that the protective agent for hypertension blood sample detection as claimed in any one of claims 1 to 4 is pre-filled in the vacuum blood collection tube for hypertension blood sample detection, and the vacuum blood collection tube for hypertension blood sample detection is directly added with blood sample without enzyme inhibitor in the using process, and is put into an ice water bath or a refrigerator for storage.
  6. 6. The vacuum blood collection tube for hypertension blood sample detection according to claim 5, wherein 34.0-41.6 mu L of the hypertension blood sample protective agent is sprayed when the specification of the vacuum blood collection tube for hypertension blood sample detection is 3mL, 56.7-69.3 mu L of the hypertension blood sample protective agent is sprayed when the specification of the vacuum blood collection tube is 5mL, and the corresponding amount of the hypertension blood sample protective agent is sprayed according to the proportion of other specifications.
  7. 7. Use of a vacuum blood collection tube for the detection of a hypertensive blood sample, characterized in that the use of a vacuum blood collection tube for the detection of a hypertensive blood sample according to claim 5 or 6 for direct preservation of blood.
  8. 8. The application of the vacuum blood collection tube for detecting the hypertension blood sample according to claim 7, wherein the ratio of the volume of the hypertension blood sample protecting agent to the volume of the blood is 1:50-100 in the process of preserving the blood.

Description

Hypertension blood sample protective agent, vacuum blood collection tube for hypertension blood sample detection and application Technical Field The invention relates to the technical field of medical equipment, in particular to a hypertension blood sample protective agent, a vacuum blood sampling tube for detecting hypertension blood samples and application thereof, and especially relates to a hypertension blood sample protective agent for detecting angiotensin I and angiotensin II, a vacuum blood sampling tube for detecting hypertension blood samples and application thereof. Background With the continuous increase of the prevalence of hypertension and the continuous increase of the mortality rate of cardiovascular and cerebrovascular diseases, people pay more attention to the prevention, detection and treatment of hypertension. The usual indicators for the current hypertension detection are plasma renin, plasma corticotropin, plasma angiotensin II, plasma aldosterone and plasma cortisol. The renin-angiotensin-aldosterone (RAAS) system is an important system for regulating water-salt metabolism and maintaining circulatory blood pressure. Renin is secreted by the juxtaglomerular cells of the renal juxtaglomerular arterioles, and catalyzes the conversion of liver-secreted angiotensinogen to angiotensin I, which is degraded in the pulmonary circulation by angiotensin converting enzymes from pulmonary epithelial cells to angiotensin II. In order to block the conversion of the angiotensin I and the angiotensin II, so that the concentrations of the angiotensin I and the angiotensin II are kept relatively stable to achieve the purpose of accurate detection, an anticoagulant, an enzyme inhibitor and a complexing agent are added into a blood collection tube after blood collection, and the conversion of the angiotensin I and the angiotensin II is prevented by a method commonly used for sample pretreatment for detecting the angiotensin I and the angiotensin II. The sample pretreatment adopted by most detection kits on the market at present is to add an inhibitor after venous blood is collected by using an anticoagulant tube and then uncap, so that the operation not only increases the detection time, but also can not standardize the blood sampling operation, and larger error is easily brought to detection. However, the conventional vacuum blood collection tube cannot provide an enzyme inhibitor for blood collection at hypertension, and therefore cannot be fully applied to detection of blood at hypertension. Disclosure of Invention The invention aims to solve the problem that the existing vacuum blood collection tube cannot be fully applied to hypertension blood detection, and provides a hypertension blood sample protecting agent, a vacuum blood collection tube for hypertension blood sample detection and application thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The hypertension blood sample protective agent comprises, by weight, 1-1.3 parts of enalapril maleate, 15.2-18.6 parts of anticoagulant and 13.8-16.9 parts of complexing agent. Further, the complexing agent is imidazolidinyl urea and 8-hydroxyquinoline sulfate. Further, the weight ratio of the imidazolidinyl urea to the 8-hydroxyquinoline sulfate is 7.5-9.2:6.3-7.7. Further, the anticoagulant is dipotassium ethylenediamine tetraacetate. Further, the preparation method of the hypertension blood sample protective agent comprises the steps of taking enalapril maleate, an anticoagulant and a complexing agent, and adding water for injection for dissolution to prepare the hypertension blood sample protective agent. Further, the hypertension blood sample protective agent contains 15.2-18.6 g/100mL of ethylene diamine tetraacetic acid dipotassium, 7.5-9.2 g/100mL of imidazolidinyl urea, 6.3-7.7 g/100mL of 8-hydroxyquinoline sulfate and 1.0-1.3 g/100mL of enalapril maleate. A vacuum blood collection tube for detecting a hypertensive blood sample, wherein the vacuum blood collection tube for detecting a hypertensive blood sample is provided with the hypertensive blood sample protecting agent. Further, 34.0-41.6 mu L of the hypertension blood sample protective agent is sprayed when the specification of the vacuum blood sampling tube for hypertension blood sample detection is 3mL, 56.7-69.3 mu L of the hypertension blood sample protective agent is sprayed when the specification is 5mL, and the corresponding amount of the hypertension blood sample protective agent is sprayed according to the proportion of other specifications. The application of the vacuum blood collection tube for detecting the hypertension blood sample is that the vacuum blood collection tube for detecting the hypertension blood sample is adopted to directly store blood. Further, in the process of preserving blood, the ratio of the hypertensive blood sample protective agent to the volume of blood is 1:50-100. The invention relates to a hyperten