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CN-119792175-B - Sturgeon caviar extract and preparation method and application thereof

CN119792175BCN 119792175 BCN119792175 BCN 119792175BCN-119792175-B

Abstract

The invention belongs to the field of daily chemicals, and discloses a preparation method of a sturgeon caviar extract. According to the invention, a Soxhlet extraction method is adopted, ethanol or diethyl ether is used as a solvent for extraction, so that the sturgeon caviar extract is obtained, the DHA content of the obtained extract is 3.1-8.1wt%, the vitamin E content is 500-1150 mug/g, the phosphatidylcholine content is 3-4.8wt%, and the content of the active ingredients is far beyond that of a enzymolysis method. The invention also aims at providing the application of the sturgeon caviar extract.

Inventors

  • WU SHENGHU
  • KANG JIAXIN
  • TAN XIAOLING
  • SUN YONG
  • PANG SHIFENG
  • Liu Longrui

Assignees

  • 珠海伊斯佳科技股份有限公司

Dates

Publication Date
20260508
Application Date
20250207

Claims (6)

  1. 1. A preparation method of a sturgeon caviar extract is characterized in that clean sturgeon caviar is filled into a filter bag, filter paper is rapidly analyzed by a lower layer pad, the specific pore diameter of the filter paper is 80 meshes, then the filter paper and the filter paper are placed in a reaction extraction device, an extraction solvent is added into a reactor to be extracted by adopting a Soxhlet extraction method, the extraction solvent is 70% ethanol, the addition amount of the extraction solvent is 9 times of the mass of the sturgeon caviar, the treatment temperature is 90 ℃, after 1.5 hours of extraction, a sturgeon caviar extract is poured into the reactor, and then concentration and sterilization treatment are carried out to obtain the sturgeon caviar extract; Before extraction, performing pretreatment on sturgeon caviar, wherein the pretreatment is one or more of cleaning, impurity removal, decolorization, deodorization, sodium removal and cleaning and filtering; The sturgeon caviar extract contains 8.07wt% DHA, 1136.61 μg/g vitamin E, 4.77wt% phosphatidylcholine, 2.1g/100g amino acid, 1.70mg/100mL vitamin B 1 , 3.39mg/100mL vitamin B 2 , 8.04mg/100mL vitamin B 6 , 309.0 μg/100g vitamin D 3 , 4480 μg/100g niacin, 95.0mg/100g taurine, 70.5 μg/100g biotin, 9.59mg/100g pantothenic acid; The sturgeon caviar extract has hyaluronidase activity inhibiting, free radical scavenging, tightening and anti-wrinkle effects.
  2. 2. The preparation method according to claim 1, wherein the pretreatment is a cleaning and impurity removal, a decoloring treatment, a deodorization treatment, a sodium removal treatment, a cleaning and filtering operation which are sequentially performed; the decolorization treatment adopts a decolorizer to treat sturgeon roes; the deodorization treatment adopts a deodorization agent to treat sturgeon roes; the sodium removal treatment adopts a sodium removal agent to treat sturgeon roes.
  3. 3. The preparation method of the composite material of the diatomite is characterized in that the decoloring agent is diatomite, the smell of the deodorizing agent is bacillus subtilis liquid, the sodium removing agent is a mixed liquid of 0.1-0.35 wt% of hydrogen peroxide, 0.05-0.2 wt% of calcium chloride, 0.05-0.2 wt% of glacial acetic acid and the balance of water, and the concentration of the hydrogen peroxide is 30wt%; The treatment temperature of the sodium removal treatment is 20-25 ℃ and the treatment time is 0.5-1.5 h; the concentration treatment takes ethanol removal as a treatment endpoint.
  4. 4. A sturgeon caviar extract prepared by the method of any one of claims 1 to 3.
  5. 5. Use of sturgeon caviar extract according to claim 4 for the preparation of cosmetics.
  6. 6. A cosmetic product, which is characterized in that, a sturgeon caviar extract according to claim 4.

Description

Sturgeon caviar extract and preparation method and application thereof Technical Field The invention relates to the field of cosmetics, in particular to a preparation method and application of sturgeon roe sauce extract. Background Most of the prior art extracts active ingredients (DHA, vitamin E, phosphatidylcholine, amino acid and the like) in sturgeon roe by using biological protease, but the extraction content is low, the extraction is insufficient, and the extraction efficiency of the method is low due to the stability and activity problems of the enzyme, so that the requirements of practical application cannot be met. In addition, the method may damage the structure of the active ingredient during extraction, further reducing the activity of the extract. The subject matter of the publication No. CN116999379B is sturgeon roe extract, a preparation method and application thereof, and the preparation method and application thereof are disclosed, wherein the preparation method comprises the following steps of (1) breaking the wall of sturgeon roe, adding enzyme for enzymolysis, wherein the enzyme adopted for enzymolysis is protease and lipase, the weight ratio of the sturgeon roe, the protease and the lipase is 50 (1.15-5.15) to 0.15, the protease is papain and trypsin, and (2) centrifuging and filtering to obtain the sturgeon roe extract. In the research process of the project, papain, alkaline protease, lecithin and neutral protease are adopted to carry out enzymolysis extraction on sturgeon roe, and the content of DHA, vitamin E and phosphatidylcholine is found to be unable to meet the ideal requirement. Disclosure of Invention The invention aims to provide a preparation method of a sturgeon caviar extract, which comprises the steps of adopting a Soxhlet extraction method and using ethanol or diethyl ether as a solvent to extract, so as to obtain the sturgeon caviar extract, wherein the DHA content of the obtained extract is 3.1-8.1wt%, the vitamin E content is 500-1150 mug/g, the phosphatidylcholine content is 3-4.8wt%, and the content of the active ingredients is far beyond that of the sturgeon caviar extract. The invention also aims at providing the application of the sturgeon caviar extract. In order to achieve the above purpose, the invention provides a preparation method of sturgeon caviar extract, which adopts Soxhlet extraction method and ethanol or diethyl ether as solvent for extraction to obtain sturgeon caviar extract. The sturgeon caviar, which is a food rich in DHA, amino acid, vitamin E, phosphatidylcholine and other active ingredients, is always an object of the extraction of the researchers. The extraction of sturgeon caviar is a challenging task due to the low levels of active ingredients obtained by the bioprotease extraction techniques provided by the literature, patents, and the like. The biological protease extraction technology mainly extracts active ingredients in sturgeon roe sauce through the catalysis of enzyme. However, this method is not efficient in extraction because of stability and activity problems of enzymes, and cannot meet the demands of practical applications. In addition, the method may damage the structure of the active ingredient during extraction, further reducing the activity of the extract. Meanwhile, according to analysis on commercially available sturgeon caviar extracts, most of the commercially available sturgeon caviar extracts are water-soluble extracts, and meanwhile, different enzymolysis methods are tried to prepare sturgeon caviar extracts, and the properties of the sturgeon caviar extracts in terms of oxidation resistance, wrinkle resistance and the like are unsatisfactory whether the sturgeon caviar extracts are commercially available products or products obtained by various enzymolysis methods. To solve this problem, the present project development has attempted a new extraction method, soxhlet extraction. The Soxhlet extraction method is a traditional organic chemical extraction technology, and utilizes a solvent to dissolve and reflux substances, so that sturgeon roe can be extracted repeatedly and fully. Soxhlet extraction has a number of advantages over the bioprotease extraction technique. First, the Soxhlet extraction method has higher extraction efficiency, and can extract more active ingredients in a short time. Secondly, the Soxhlet extraction method does not destroy the structure of the active ingredient during the extraction process, so that the activity of the extract can be ensured. Finally, the Soxhlet extraction process is relatively simple to operate, requiring no complicated equipment and high costs. In the preparation method, the weight of the ethanol or the diethyl ether is 8-10 times of the weight of the sturgeon roe. The preparation method comprises the steps of extracting at 80-100 ℃, extracting for 1-2 hours, placing sturgeon seeds in a filter bag for extraction, wherein the mesh number of the filter bag is 80-100. In the p