CN-119804604-B - Biosensor array monitoring system and method for plant organic small molecule distribution imaging

CN119804604BCN 119804604 BCN119804604 BCN 119804604BCN-119804604-B

Abstract

The invention relates to a biosensor array monitoring system and a method for plant organic micromolecule distribution imaging, wherein the system comprises a biosensor array and a control module, the control module comprises a signal output and input conditioning unit and a control and data processing unit, the biosensor array is used for being coupled with a living plant sample, current signals caused by corresponding organic micromolecules are collected based on an applied linear scanning voltage signal, the signal output and input conditioning unit is used for applying the linear scanning voltage signal to the biosensor array and conditioning the current signals collected by the biosensor array to obtain voltage signals, the control and data processing unit is used for calibrating the biosensor array and the signal output and input conditioning unit to generate the linear scanning voltage signals and analyzing and processing the obtained voltage signals to obtain living in-situ distribution images of the organic micromolecules, and the biosensor array monitoring system can be widely applied to the sensor technology agricultural application field.

Inventors

WANG ZHONGYI
WANG ZHIHAO
FAN LIFENG
WANG CHAO
HUANG LAN

Assignees

中国农业大学

Dates

Publication Date: 20260505
Application Date: 20241226

Claims (8)

1. The plant small organic molecule distribution imaging biosensor array monitoring system is characterized by comprising a biosensor array and a control module, wherein the biosensor array comprises a plurality of biosensors, and the control module comprises a signal output and input conditioning unit and a control and data processing unit; The biosensor array is used for coupling with a living plant sample, outputting and inputting linear scanning voltage signals applied by the conditioning unit based on the signals, respectively detecting the concentration of the organic small molecules at the corresponding positions, and collecting current signals caused by the corresponding organic small molecules; the signal output and input conditioning unit is used for applying the linear scanning voltage signal generated by the control and data processing unit to the biosensor array and conditioning the current signal acquired by the biosensor array to obtain a voltage signal; the control and data processing unit is used for calibrating the biosensor array and the signal output and input conditioning unit, generating a linear scanning voltage signal, and analyzing and processing the obtained voltage signal to obtain an in-situ distribution image of the living body of the small organic molecules; The biosensor array comprises more than 6 specific organic small molecules of biosensors, and each biosensor comprises a working electrode, an auxiliary electrode and a reference electrode; The reference electrode is composed of silver nanowires and polydimethylsiloxane, the auxiliary electrode is composed of poly (3, 4-ethylenedioxythiophene) -polystyrene sulfonate and polyimide, and the working electrode is composed of silver nanowires modified by graphene and perfluorinated sulfonic acid resin.
2. The plant small organic molecule distributed imaging biosensor array monitoring system according to claim 1, wherein the signal output and input conditioning unit comprises: signal excitation device for applying the linear scanning voltage signal generated by the control and data processing unit between each working electrode and auxiliary electrode in the biosensor array to make the electrodes produce electrochemical reaction; The signal acquisition device is used for acquiring current signals on each working electrode in the biosensor array, namely current signals caused by corresponding small organic molecules, and converting the current signals into voltage signals.
3. The plant small organic molecule distributed imaging biosensor array monitoring system of claim 2, wherein the signal acquisition device comprises: The programmable amplifier is used for collecting current signals on each working electrode in the biosensor array, and the amplification gain of the amplifier is adjusted through programming so as to match different signal sources, so that the signal collection parameters of the optimal amplifier are determined; and the current measurement conditioning circuit is used for converting a current signal caused by the small organic molecules into a voltage signal and amplifying the voltage signal.
4. A plant small organic molecule distributed imaging biosensor array monitoring system according to claim 2, wherein said control and data processing unit comprises: the analog-digital converter is used for converting the voltage signal conditioned by the signal acquisition device into a digital signal and inputting the digital signal to the microcontroller; The digital-analog converter is used for generating a linear scanning voltage signal and sending the linear scanning voltage signal to the signal excitation device; the microcontroller is used for reversely calculating an original current signal based on the digital signal, performing imaging processing, and outputting a living body in-situ distribution image of the corresponding organic micromolecules by using the pseudo-color image.
5. A plant small organic molecule distribution imaging based biosensor array monitoring method of the plant small organic molecule distribution imaging based biosensor array monitoring system of any one of claim 1 to 4, characterized by comprising the following steps: The control and data processing unit calibrates the biosensor array and the signal output and input conditioning unit; Coupling the biosensor array with a living plant sample, generating a linear scanning voltage signal by a control and data processing unit, and applying the linear scanning voltage signal to the biosensor array through a calibrated signal output and input conditioning unit; the biosensor array collects current signals caused by corresponding small organic molecules based on the applied linear scanning voltage signals; The calibrated signal output and input conditioning unit conditions the current signals acquired by the biosensor array to obtain voltage signals, and the obtained voltage signals are sent to the control and data processing unit; The control and data processing unit analyzes and processes the obtained voltage signals to obtain in-situ distribution images of living bodies of small organic molecules.
6. The method for monitoring the plant small organic molecule distribution imaging biosensor array according to claim 5, wherein the preparation process of the biosensor array is as follows: adopting silver nanowires and polydimethylsiloxane to form a reference electrode of the biosensor; the auxiliary electrode of the biosensor is formed by poly (3, 4-ethylenedioxythiophene) -polystyrene sulfonate and polyimide; forming a working electrode of the biosensor by adopting graphene and a silver nanowire modified by perfluorinated sulfonic acid resin to obtain the biosensor; based on the resulting biosensor, a biosensor array is constructed.
7. The method for monitoring the biosensor array by using the plant small organic molecule (PLMN) imaging according to claim 5, wherein the control and data processing unit calibrates the biosensor array and the signal output and input conditioning unit, and comprises the following steps: the control and data processing unit calibrates the signal output and input conditioning unit: judging the current signals collected by the biosensor array by adopting a standard resistance-capacitance model to calibrate the output and input conditioning unit, confirming whether the output and input conditioning unit works normally or not, and if the collected current signals are in the estimated range, considering that the output and input conditioning unit works normally; the control and data processing unit calibrates the biosensor array: Calibrating each biosensor in the biosensor array by using the prepared m standard concentration solutions of the to-be-detected substances, smoothly filtering the volt-ampere characteristic curve of each biosensor in the biosensor array, and determining the peak point of each volt-ampere characteristic curve by using a first derivative algorithm; Each biosensor measures peak values in m solutions with standard concentrations, establishes a unitary regression model, and stores two coefficients of the unitary regression model as correction parameters for standby.
8. The method for monitoring the biosensor array by using the plant small organic molecule distribution imaging according to claim 7, wherein the control and data processing unit analyzes and processes the obtained voltage signal to obtain an in-situ distribution image of the small organic molecule, and the method comprises the following steps: The analog-digital converter converts the conditioned voltage signal into a digital signal and inputs the digital signal to the microcontroller; the microcontroller obtains an original current signal based on the digital signal through back calculation; the obtained original current signal is carried into a unitary regression model to obtain concentration information of small molecules to be detected in a living plant sample; And (3) carrying out imaging processing on the original current signal, and outputting a living body in-situ distribution image corresponding to the organic small molecules by using the pseudo-color image.

Description

Biosensor array monitoring system and method for plant organic small molecule distribution imaging Technical Field The invention relates to the field of agricultural application of sensor technology, in particular to a biosensor array monitoring system and method for plant organic small molecule distribution imaging. Background In the plant growth process, the method of adopting a multifunctional and miniaturized sensor is used for continuously monitoring the dynamic changes of small molecules and ions in the plant body, and has important significance in the aspects of early plant disease detection, stress sensing, growth prediction and the like. Currently, most wearable sensors can only detect physical growth of plants or atmospheric environmental parameters. Thus, there is a need for a multifunctional, real-time sensing method and system to achieve high sensitivity, high specificity to track the distribution of biochemical signals of plants (e.g., small molecular organic compounds within the plant body, such as hormones within the plant body, etc.). For small molecule organic compounds in plants, the prior art is directed to single point measurement of small molecule organic compounds in plants. However, to master early disease detection, stress sensing and growth laws of plants, it is more important to monitor the signal distribution of small molecular organic compounds (e.g., hormones in plants, etc.), but it is also more challenging, for example, to deal with the signal-to-noise ratio, synchronization of sensor arrays, and especially the problem of mutual interference during measurement. However, there is no system capable of realizing high sensitivity, high selectivity and real-time monitoring on the distribution of small organic molecules of plants in the prior art. Disclosure of Invention Aiming at the problems, the invention aims to provide a biosensor array monitoring system and a biosensor array monitoring method for plant organic small molecule distribution imaging, which can realize high-sensitivity, high-selectivity and real-time monitoring of plant organic small molecule distribution. In order to achieve the aim, the invention adopts the following technical scheme that on one hand, a plant organic small molecular distribution imaging biosensor array monitoring system is provided, which comprises a biosensor array and a control module, wherein the biosensor array comprises a plurality of biosensors, and the control module comprises a signal output and input conditioning unit and a control and data processing unit; The biosensor array is used for coupling with a living plant sample, outputting and inputting linear scanning voltage signals applied by the conditioning unit based on the signals, respectively detecting the concentration of the organic small molecules at the corresponding positions, and collecting current signals caused by the corresponding organic small molecules; the signal output and input conditioning unit is used for applying the linear scanning voltage signal generated by the control and data processing unit to the biosensor array and conditioning the current signal acquired by the biosensor array to obtain a voltage signal; The control and data processing unit is used for calibrating the biosensor array and the signal output and input conditioning unit, generating a linear scanning voltage signal, and analyzing and processing the obtained voltage signal to obtain an in-situ distribution image of the living body of the small organic molecules. Further, the biosensor array comprises more than 6 specific small organic molecules of the biosensor, and each biosensor comprises three electrodes, namely a working electrode, an auxiliary electrode and a reference electrode. Further, the reference electrode is composed of silver nanowires and polydimethylsiloxane, the auxiliary electrode is composed of poly (3, 4-ethylenedioxythiophene) -polystyrene sulfonate and polyimide, and the working electrode is composed of graphene and perfluorinated sulfonic acid resin modified silver nanowires. Further, the signal output and input conditioning unit includes: signal excitation device for applying the linear scanning voltage signal generated by the control and data processing unit between each working electrode and auxiliary electrode in the biosensor array to make the electrodes produce electrochemical reaction; The signal acquisition device is used for acquiring current signals on each working electrode in the biosensor array, namely current signals caused by corresponding small organic molecules, and converting the current signals into voltage signals. Further, the signal acquisition device includes: The programmable amplifier is used for collecting current signals on each working electrode in the biosensor array, and the amplification gain of the amplifier is adjusted through programming so as to match different signal sources, so that the signal collection parameters of the optimal amplifi