CN-119824000-B - NtMIXTA-like gene, coded protein thereof, biological material and application thereof in plant glandular hair growth regulation

Abstract

The application discloses NtMIXTA-like genes, coded proteins and biological materials thereof and application thereof in plant glandular hair growth regulation and control, and aims to solve the technical problem of single function of the existing glandular hair regulation and control genes. According to the application, ntMIXTA-like genes are cloned from tobacco, knock-out and over-expression plants of NtMIXTA-like genes are respectively constructed, and analysis of leaf surface glandular hairs of the plants shows that the density of the glandular hairs is obviously increased after NtMIXTA-like over-expression, which indicates that NtMIXTA-like genes can positively regulate and control the growth/development of the glandular hairs of tobacco. The application has important significance for researching a molecular regulation network of plant glandular hair.

Inventors

• WANG ZHAOJUN
• WANG SHUAI
• CUI HONG
• YAN XIAOXIAO
• ZHANG HONGYING

Assignees

• 河南农业大学

Dates

Publication Date

20260508

Application Date

20241224

Claims (2)

1. 1. The NtMIXTA-like gene with the nucleotide sequence shown in SEQ ID NO.1, the coded protein thereof or the biological material containing the gene is applied to any one of the following (1) - (4): (1) Application of the agent for positively regulating plant glandular hair generation/development or preparing the agent for positively regulating plant glandular hair generation/development; (2) The application of the plant glandular hair density character positive regulation or the preparation of the reagent for the plant glandular hair density character positive regulation; (3) The application of breeding and identifying varieties/lines related to plant glandular wool density traits; (4) The application of the transgenic plant with increased glandular wool density in construction or breeding; The method is characterized in that the density of glandular hairs can be improved by over-expressing or up-regulating the NtMIXTA-like gene, the glandular hairs of plants are at least one of long-handled glandular hairs and short-handled glandular hairs, the plants are tobacco, and the biological material is any one of nucleic acid molecules, over-expression vectors, host cells and engineering bacteria.
2. 2. A method for increasing the glandular wool density of tobacco, comprising the steps of: (1) Constructing an over-expression vector containing NtMIXTA-like genes with nucleotide sequences shown as SEQ ID NO. 1; (2) Transforming the over-expression vector into tobacco tissue or cells; (3) The NtMIXTA-like gene is overexpressed in tobacco tissue or cells.

Description

NtMIXTA-like gene, coded protein thereof, biological material and application thereof in plant glandular hair growth regulation Technical Field The invention relates to the technical field of genetic engineering breeding, in particular to NtMIXTA-like genes, coded proteins and biological materials thereof and application thereof in plant glandular hair growth regulation. Background Many plants (e.g., tobacco, etc.) are surface-coated with a large amount of epidermal hair, with the secretory function of the epidermal hair being called glandular hair. The tobacco glandular hair is further divided into long glandular hair and short glandular hair according to the difference of the number of the stem cells, the long glandular hair is provided with a plurality of stem cells and can synthesize and release secondary metabolites such as diterpenoids and sucrose esters, and the short glandular hair only contains one stem cell and is responsible for synthesizing and secreting protein Phylloplain with antifungal property. The tobacco glandular hair has important significance in enhancing the environmental adaptability of tobacco plants and improving the aroma quality of tobacco leaves. Firstly, the chemical substances secreted by the tobacco glandular hairs have strong biological activity, not only have poisoning and expelling effects on pests, but also the resistant proteins such as Phylloplain in the chemical substances can obviously reduce the probability of the tobacco suffering from fungal diseases, and further improve the disease resistance of the tobacco plants. More importantly, the secretions are also rich in diterpenoid, sucrose ester and other aroma precursor substances, and the precursor substances can be gradually degraded and converted into micromolecular volatile components during the processing, aging and burning processes of tobacco leaves, and the components are important bases for the unique aroma of the tobacco leaves. Therefore, the improvement of the glandular hair density of the tobacco is of great importance, however, the direct screening of the glandular hair density in the field is difficult due to the tiny size of the tobacco glandular hair Mao Xingtai, and the traditional breeding method faces great challenges in the aspect of breeding the high glandular hair density tobacco variety. Deep excavation of gene for regulating glandular hair density, and utilizing gene engineering and other modern biotechnology means to produce new tobacco variety with high glandular hair density. Although some genes which regulate the development of glandular hairs, such as NtCycB, ntHD, ntHD, etc., have been found in tobacco, these genes are involved in the regulation of long-handled glandular hairs only and have no effect on the density of short-handled glandular hairs. Therefore, the gene capable of simultaneously regulating and controlling the density of the long-handled glandular hairs and the short-handled glandular hairs of the tobacco is searched, and the gene has extremely high application value for breeding new varieties of the tobacco with high glandular hairs density. The information disclosed in this background section is only for enhancement of understanding of the background of the disclosure and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art that is well known to a person skilled in the art. Disclosure of Invention In view of at least one of the above technical problems, the present disclosure provides a NtMIXTA-like gene, its coding protein, biological material and application in plant glandular hair generation regulation, aiming at solving the technical problem that the existing glandular hair regulation gene has single function (for example, only participates in regulation of long-handled glandular hair and has no influence on density of short-handled glandular hair). According to a first aspect of the present disclosure, there is provided a NtMIXTA-like gene whose nucleotide sequence is shown as SEQ ID NO. 1. According to a second aspect of the present disclosure, there is provided a protein encoded by the NtMIXTA-like gene, the amino acid sequence of which is shown in SEQ ID NO. 2. According to a third aspect of the present disclosure, there is provided a biomaterial comprising the NtMIXTA-like gene or a fragment thereof. In some embodiments of the disclosure, the biological material is any one of a nucleic acid molecule, an over-expression vector, a host cell, an engineering bacterium, a transformed plant cell. According to a fourth aspect of the present disclosure, the NtMIXTA-like gene, the encoded protein, or the biological material is used in any one of the following (1) to (7): (1) Application of the agent for positively regulating plant glandular hair generation/development or preparing the agent for positively regulating plant glandular hair generation/development; (2) The application of the plant glandular hair density