CN-119842095-B - Vinyl trimethyl silane modified gelatin and preparation method and application thereof

CN119842095BCN 119842095 BCN119842095 BCN 119842095BCN-119842095-B

Abstract

The invention provides vinyl trimethyl silane modified gelatin, a preparation method and application thereof, which belong to the technical field of gelatin modification, wherein vinyl trimethyl silane is adopted to modify gelatin, and the modified gelatin has the advantages of stronger hydrophobicity, oxidation resistance and the like, also has better external force resistance, obviously improves toughness and rigidity, can promote cell migration, has the cell migration rate of 60-65.4% of epidermal cells, and accelerates healing of skin wound surfaces.

Inventors

XU JING
YANG YAOZHEN
WANG YONGLE
ZHANG XIAOHAN
LI TIANDUO
WANG LING
SUN XINYU
WU YANJUAN

Assignees

齐鲁工业大学(山东省科学院)

Dates

Publication Date: 20260505
Application Date: 20231018

Claims (10)

1. The vinyltrimethylsilane modified gelatin is characterized in that the contact angle of the vinyltrimethylsilane modified gelatin is 90-98 degrees, the clearance rate of hydroxyl radicals is 90-98.6%, the clearance rate of DPPH radicals is 73-80.4%, the clearance rate of superoxide radicals is 85-91.5%, the cell mobility of epidermal cells is 60-65.4%, the tensile strength of the vinyltrimethylsilane modified gelatin is increased by 19.3% compared with the gelatin before modification, the Young modulus is increased by 14.8% compared with the gelatin before modification, and the elongation at break is increased by 25.2% compared with the gelatin before modification; The preparation method of the vinyltrimethylsilane modified gelatin specifically comprises the following steps: (1) Preparing a gelatin solution with the weight of 3-5%, standing at room temperature, placing the gelatin solution into a water bath with the temperature of 42-48 ℃ and stirring for 10-15 min, and adjusting the pH value to 9-10; (2) Adding tetrabutylammonium iodide into the gelatin solution in the step (1) under the condition of isolating oxygen to obtain a reactant 1; (3) Dissolving vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, adding the vinyltrimethylsilane solution into the reactant 1, and fully mixing to obtain a reactant 2; (4) Preparing dimethyl sulfoxide and water into a mixed solvent according to a ratio of 5-7:4-5 (v/v), dissolving triethylamine in the mixed solvent to obtain a triethylamine solution, adding the triethylamine solution into a reactant 2, and stirring and reacting for 40-50 h at 42-48 ℃ to obtain a reactant 3; (5) Pouring the reactant 3 into a mold, drying the reactant in a baking oven at 40-45 ℃ under normal pressure, leaching the reactant by using acetone, and drying the reactant again to obtain the vinyltrimethylsilane modified gelatin.
2. The vinyltrimethylsilane modified gelatin of claim 1, wherein the infrared spectrum of the vinyltrimethylsilane modified gelatin exhibits a Si-O characteristic peak at 1140cm -1 and an amide II band of N-H bond flexural vibration at 3110cm -1 , a telescopic vibration peak of C-H bond in an olefin at 3020cm -1 , an amide II band of N-H bond flexural vibration at 1590cm -1 , an amide III band of C-N bond telescopic vibration at 1480cm -1 , a symmetrical telescopic vibration peak of methyl C-H bond at 1360cm -1 ,1300cm -1 , a bending vibration peak of C-H bond at 979cm -1 .
3. The vinyltrimethylsilane-modified gelatin according to claim 1, wherein the standing time at room temperature in said step (1) is 1h or more.
4. The vinyltrimethylsilane modified gelatin according to claim 1, wherein the oxygen separation in step (2) is performed by continuously introducing nitrogen gas into the gelatin solution in step (1) for 10 minutes, and then adding tetrabutylammonium iodide, and continuously introducing nitrogen gas for 30 minutes.
5. The vinyltrimethylsilane modified gelatin of claim 1, wherein the weight ratio of gelatin, tetrabutylammonium iodide, vinyltrimethylsilane, triethylamine is 30-40:1:5:1.
6. Use of vinyltrimethylsilane modified gelatin as defined in claim 1 in the preparation of a skin care product.
7. The use according to claim 6, wherein the skin care product comprises a lotion, a cream, a moisturizing cream, a hand cream, a foot cream, a bath product, a shampoo product, a hair care product, and the skin care product comprises a water, an emulsion, a cream, a gel, a mask product.
8. The application of the vinyl trimethylsilane is characterized in that the vinyl trimethylsilane is applied to improving the tensile strength of gelatin, and the application method is as follows: (1) Preparing a gelatin solution with the weight of 3-5%, standing at room temperature, placing the gelatin solution into a water bath with the temperature of 42-48 ℃ and stirring for 10-15 min, and adjusting the pH value to 9-10; (2) Adding tetrabutylammonium iodide into the gelatin solution in the step (1) under the condition of isolating oxygen to obtain a reactant 1; (3) Dissolving vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, adding the vinyltrimethylsilane solution into the reactant 1, and fully mixing to obtain a reactant 2; (4) Preparing dimethyl sulfoxide and water into a mixed solvent according to a ratio of 5-7:4-5 (v/v), dissolving triethylamine in the mixed solvent to obtain a triethylamine solution, adding the triethylamine solution into a reactant 2, and stirring and reacting for 40-50 h at 42-48 ℃ to obtain a reactant 3; (5) Pouring the reactant 3 into a mold, drying the reactant in a baking oven at 40-45 ℃ under normal pressure, leaching the reactant with acetone, and drying the reactant again to obtain vinyltrimethylsilane modified gelatin; The contact angle of the vinyltrimethylsilane modified gelatin is 90-98 degrees, the clearance rate of hydroxyl radicals is 90-98.6%, the clearance rate of DPPH radicals is 73-80.4%, the clearance rate of superoxide radicals is 85-91.5%, the cell mobility of the epidermal cells is 60-65.4%, the tensile strength of the vinyltrimethylsilane modified gelatin is increased by 19.3% compared with the gelatin before modification, the Young modulus is increased by 14.8% compared with the gelatin before modification, and the elongation at break is increased by 25.2% compared with the gelatin before modification.
9. The application of the vinyltrimethylsilane is characterized in that the vinyltrimethylsilane is applied to improving the oxidation resistance of gelatin, and the application method is as follows: (1) Preparing a gelatin solution with the weight of 3-5%, standing at room temperature, placing the gelatin solution into a water bath with the temperature of 42-48 ℃ and stirring for 10-15 min, and adjusting the pH value to 9-10; (2) Adding tetrabutylammonium iodide into the gelatin solution in the step (1) under the condition of isolating oxygen to obtain a reactant 1; (3) Dissolving vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, adding the vinyltrimethylsilane solution into the reactant 1, and fully mixing to obtain a reactant 2; (4) Preparing dimethyl sulfoxide and water into a mixed solvent according to a ratio of 5-7:4-5 (v/v), dissolving triethylamine in the mixed solvent to obtain a triethylamine solution, adding the triethylamine solution into a reactant 2, and stirring and reacting for 40-50 h at 42-48 ℃ to obtain a reactant 3; (5) Pouring the reactant 3 into a mold, drying the reactant in a baking oven at 40-45 ℃ under normal pressure, leaching the reactant with acetone, and drying the reactant again to obtain vinyltrimethylsilane modified gelatin; The contact angle of the vinyltrimethylsilane modified gelatin is 90-98 degrees, the clearance rate of hydroxyl radicals is 90-98.6%, the clearance rate of DPPH radicals is 73-80.4%, the clearance rate of superoxide radicals is 85-91.5%, the cell mobility of the epidermal cells is 60-65.4%, the tensile strength of the vinyltrimethylsilane modified gelatin is increased by 19.3% compared with the gelatin before modification, the Young modulus is increased by 14.8% compared with the gelatin before modification, and the elongation at break is increased by 25.2% compared with the gelatin before modification.
10. The application of the vinyltrimethylsilane is characterized in that the vinyltrimethylsilane is applied to improving the hydrophobicity of gelatin, and the application method is as follows: (1) Preparing a gelatin solution with the weight of 3-5%, standing at room temperature, placing the gelatin solution into a water bath with the temperature of 42-48 ℃ and stirring for 10-15 min, and adjusting the pH value to 9-10; (2) Adding tetrabutylammonium iodide into the gelatin solution in the step (1) under the condition of isolating oxygen to obtain a reactant 1; (3) Dissolving vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, adding the vinyltrimethylsilane solution into the reactant 1, and fully mixing to obtain a reactant 2; (4) Preparing dimethyl sulfoxide and water into a mixed solvent according to a ratio of 5-7:4-5 (v/v), dissolving triethylamine in the mixed solvent to obtain a triethylamine solution, adding the triethylamine solution into a reactant 2, and stirring and reacting for 40-50 h at 42-48 ℃ to obtain a reactant 3; (5) Pouring the reactant 3 into a mold, drying the reactant in a baking oven at 40-45 ℃ under normal pressure, leaching the reactant with acetone, and drying the reactant again to obtain vinyltrimethylsilane modified gelatin; The contact angle of the vinyltrimethylsilane modified gelatin is 90-98 degrees, the clearance rate of hydroxyl radicals is 90-98.6%, the clearance rate of DPPH radicals is 73-80.4%, the clearance rate of superoxide radicals is 85-91.5%, the cell mobility of the epidermal cells is 60-65.4%, the tensile strength of the vinyltrimethylsilane modified gelatin is increased by 19.3% compared with the gelatin before modification, the Young modulus is increased by 14.8% compared with the gelatin before modification, and the elongation at break is increased by 25.2% compared with the gelatin before modification.

Description

Vinyl trimethyl silane modified gelatin and preparation method and application thereof Technical Field The invention relates to the technical field of biology, in particular to vinyl trimethyl silane modified gelatin, and a preparation method and application thereof. Background Gelatin is a derivative of collagen, the major structure and connective protein of animals, derived from collagen denaturation, containing polypeptide sequences with Gly-X-Y repeats, where X and Y are most commonly proline and hydroxyproline residues, which form triple-chain helical structures and affect the gelation ability of gelatin polypeptides, presently available gelatin being extracted by animal fur and bone processing, typically of bovine and porcine origin. The biophysical property of gelatin makes it a versatile material, and thus is widely used in the fields of medicine, imaging, industry, cosmetics, food and beverage, etc., as a valuable and versatile product. In order to obtain gelatin products with more excellent performance, a plurality of reports are reported to record a gelatin modification or optimization method, wherein the invention patent application with the publication number of CN107519035A (application number: 201710766008.2) discloses application of a gelatin-hydrolyzed gelatin compound in skin care products, and the compound has more permeability to skin after being added into cosmetics due to the similarity of gelatin and protein structures, can promote synthesis of collagen in skin, supplement collagen of skin and achieve the effects of skin health and elasticity recovery. The invention patent with publication number of CN107513173A (application number of 201710731344.3) discloses a preparation method of an organosilicon modified collagen film, which comprises the steps of firstly completely dissolving animal collagen with acetic acid solution to prepare a collagen solution, removing bubbles, casting to form a film, drying to obtain a white transparent collagen film, then placing the prepared collagen film into an inorganic alkali solution at normal temperature, adding organosilicon to react, and washing the surface after finishing, and drying to obtain the modified collagen film. Both patents describe related compounding or modification methods of gelatin, so that the modified gelatin is obviously improved in skin care and the like, but the methods and applications related to gelatin modification are still less described at present. Vinyl trimethyl silane is colorless transparent liquid, has the smell of ester, has obvious effect in modern medicine synthesis, can be used in ethylene equivalent electrophilic substitution reaction and addition reaction, and can also be used for preparing different unsaturated aryl derivatives, silicon etherification reagent, unsaturated aldehyde, chiral three-membered ring synthesis, stereoselective hydrogenation and the like. In addition, vinyl trimethyl silane is a silane coupling agent, which is a compound composed of organic groups and silicon, different reactive groups capable of being respectively chemically combined with inorganic materials and organic materials are arranged in molecules, and the silane coupling agent is used for material modification, so that the compatibility between inorganic fillers and high-molecular organic materials can be increased, and the performance of the composite material is greatly improved. For example, in the invention patent with publication number of CN104945563A (application number: 201510406043.4), vinyl trimethylsilane is introduced for grafting by a chlorination in-situ grafting method, a dispersing aid agent is used for enabling the reaction to be more complete, and PVC chlorination in-situ grafting reaction is adopted, so that compared with a general graft copolymer, the vinyl trimethylsilane can further improve the polarity and comprehensive performance of the polymer, and the mechanical property and heat resistance of the modified PVC resin are also improved. In order to further explore more applications of vinyltrimethylsilane and more modification methods of gelatin, the invention aims to modify gelatin by using vinyltrimethylsilane, and find more potential applications of vinyltrimethylsilane in different fields. Disclosure of Invention Aiming at the problems, the invention provides the vinyltrimethylsilane modified gelatin, and the preparation method and application thereof, wherein vinyltrimethylsilane is adopted to modify gelatin, and the modified gelatin has the advantages of stronger hydrophobicity, oxidation resistance and the like, also has better external force resistance, obviously improves toughness and rigidity, can promote cell migration, has the cell migration rate of 60-65.4% to the epidermal cells, and accelerates the healing process of skin wound surfaces. The technical scheme of the invention is as follows: A vinyltrimethylsilane modified gelatin is prepared by modifying gelatin with vinyltrimethylsilan