CN-119965312-B - Plant synergistic microorganism power generation method and device

CN119965312BCN 119965312 BCN119965312 BCN 119965312BCN-119965312-B

Abstract

The invention discloses a plant synergistic microorganism power generation method, which comprises the steps of preparing a membrane electrode, preparing plant materials, coupling the electrode with plant tissues, connecting a reference electrode, connecting a circuit, inoculating microorganisms, enhancing the activity of microorganisms, starting a fuel cell and the like. Secondly, the invention expands the variety range of the couplable plants. In addition, the microporous filter membrane is innovatively introduced between the membrane electrode and the plant tissue, so that the contact and the material exchange between the electrode and the plant tissue are optimized, and the performance of the electrode and the power generation efficiency of the fuel cell are improved.

Inventors

LU ZHIHAO
HUANG HUITING
Xie wanghong
QIU YANNAN
YIN DI
ZHANG LEHUA
YANG ZHENGWU
WANG KE
ZHANG XINWAN
MENG GUANGYUAN
SHI YAQI
HU HUAWEI
YAO HAN
XIAO TAO

Assignees

华东理工大学

Dates

Publication Date: 20260508
Application Date: 20250123

Claims (10)

1. The plant synergistic microorganism power generation method is characterized by comprising the following steps of: (1) Preparing a membrane electrode, namely placing a first conductive carbon material, an ion exchange membrane and a second conductive carbon material in sequence, and then performing hot pressing to prepare the membrane electrode; (2) Preparing plant material by cutting the epidermis of the stem or root of the plant to form a bare plant tissue area, and covering the surface of the bare plant tissue area with a microporous filter membrane; (3) The electrode is coupled with plant tissue, namely the membrane electrode is attached to the plant tissue area covered with the microporous filter membrane, and a silica gel pad is covered on the cathode surface of the membrane electrode to fix the membrane electrode; (4) Preparing two syringe needles and a Luggin capillary, connecting one end of the Luggin capillary with the reference electrode, embedding the other end of the Luggin capillary and the two syringe needles between a bare plant tissue area and an anode of a membrane electrode, and sealing and fixing the embedded Luggin capillary and the embedded syringe needles through silica gel; (5) The circuit connection is that the anode and the cathode of the membrane electrode and the load of an external circuit are connected by titanium wires so that the current generated by the membrane electrode can flow to the load; (6) Microorganism inoculation, namely injecting electrochemical active microorganism strains between the exposed plant tissue area and the anode of the membrane electrode through a syringe needle to inoculate, and sealing the needle by a rubber plug after inoculation; (7) Enhancing microbial activity by injecting a concentration of a solution containing an organic carbon source metabolizable by electrochemically active microorganisms into the anode of the membrane electrode to enhance microbial activity in the anode region; (8) And (3) starting the fuel cell, namely finishing the starting of the fuel cell for generating electricity by the plant and the microorganism after the microorganism inoculation in the step (6) is continuously carried out for 2-4 days.
2. The plant synergistic microbial power generation method as claimed in claim 1, wherein: In the step (1), the first conductive carbon material is one of carbon paper, carbon felt, carbon fiber or graphite, and the second conductive carbon material is one of carbon paper, carbon felt, carbon fiber or graphite; in the step (1), the ion exchange membrane is one of a cation exchange membrane, an anion exchange membrane or a proton exchange membrane.
3. The plant synergistic microbial power generation method as claimed in claim 2, wherein: The first conductive carbon material and the second conductive carbon material are platinum-modified materials, nanoparticle-modified materials or other chemical agent-modified materials.
4. The plant synergistic microbial power generation method as claimed in claim 1, wherein: In the step (1), the temperature during hot pressing is 100-180 ℃, the pressure during hot pressing is 6-9 MPa, and the hot pressing time is 1-20 min.
5. The plant synergistic microbial power generation method as claimed in claim 1, wherein: In step (2), the bare plant tissue area comprises xylem, phloem, inner bark, root, and branch and leaf inner plant cells.
6. The plant synergistic microbial power generation method as claimed in claim 1, wherein: in the step (4), the outer parts of the two syringe needles are dipped with polyvinyl chloride materials.
7. The plant synergistic microbial power generation method as claimed in claim 1, wherein: in the step (4), the reference electrode is one of an Ag/AgX composite reference electrode or a saturated calomel reference electrode.
8. The plant synergistic microbial power generation method as claimed in claim 1, wherein: in step (6), the electrochemically active microbial species include genus Geobacillus and genus Shewanella.
9. The plant synergistic microbial power generation method as claimed in claim 1, wherein: In the step (7), the solution containing the organic carbon source which can be metabolized by the electrochemically active microorganism with a certain concentration is one of physiological saline containing 0.3-1.5 g L -1 glucose, physiological saline containing 0.3-1.5 g L -1 fructose or physiological saline containing 0.3-1.5 g L -1 lactic acid.
10. A plant-assisted microbial power plant for use in carrying out a plant-assisted microbial power generation method according to any one of claims 1 to 9, said plant comprising: A test plant having an exposed plant tissue area covered with a microporous filter membrane; A membrane electrode connected to the bare plant tissue area covered with microporous filter membrane, the membrane electrode comprising an anode, an ion exchange membrane and a cathode; a reference electrode connected between the bare plant tissue area and the anode by a Luggin capillary; The load is connected with the anode and the cathode through leads; wherein microorganisms are inoculated by inserting two syringe needles between the bare plant tissue area and the anode.

Description

Plant synergistic microorganism power generation method and device Technical Field The invention relates to the technical field of microbial fuel cells, in particular to a plant synergistic microbial power generation method and device. Background In the current society, along with the rapid development of industrialization and town, the energy consumption is increased sharply, and the traditional fossil energy structure can meet the energy requirement and simultaneously bring serious environmental pollution and climate change problems. To address this challenge, the development and utilization of clean energy has become a global focus of attention. A plant is an organism capable of converting light energy into chemical energy through photosynthesis, and organic matter stored in the body has great energy development potential. However, most of the current methods for utilizing plant energy require complex secondary transformation processes, such as high-temperature high-pressure pyrolysis, chemical treatment, composting and the like, which not only have low energy conversion efficiency, but also consume a large amount of power and energy in the transformation process, and simultaneously have certain negative effects on grains, cultivated lands, forest areas and agricultural resource environments. The microbial fuel cell is used as an emerging energy conversion device, can utilize microorganisms as catalysts to directly convert organic substrates into electric energy, and has the advantages of high efficiency, environmental protection and the like. The plant and the microbial fuel cell are combined to construct a plant-microbial fuel cell system, so that the direct utilization of organic matters in the plant can be realized, and various defects of the traditional method are avoided. However, most of the existing plant-microorganism fuel cell systems adopt a mode of coupling electrochemical active microorganisms with plant root systems, and the mode is mainly suitable for aquatic plants such as rice, calamus, sweet grass, moss and the like, and has great limitation on wide land plants. In addition, the root system coupling mode belongs to the coupling outside the plant body, is easily influenced by environmental factors such as moisture, temperature, illumination, humidity and salinity, and causes unstable operation of the plant-microorganism fuel cell and larger fluctuation of power output. Disclosure of Invention The invention aims to solve the technical problems and provide a plant synergistic microorganism power generation method, which not only expands the variety range of the couplable plants, but also improves the power generation efficiency and reliability. In order to solve the problems, the invention is realized according to the following technical scheme: In a first aspect, the present invention provides a plant synergistic microbial power generation method comprising the steps of: (1) Preparing a membrane electrode, namely placing a first conductive carbon material, an ion exchange membrane and a second conductive carbon material in sequence, and then performing hot pressing to prepare the membrane electrode; (2) Preparing plant material by cutting the epidermis of the stem or root of the plant to form a bare plant tissue area, and covering the surface of the bare plant tissue area with a microporous filter membrane; (3) The electrode is coupled with plant tissue, namely the membrane electrode is attached to the plant tissue area covered with the microporous filter membrane, and a silica gel pad is covered on the cathode surface of the membrane electrode to fix the membrane electrode; (4) Preparing two syringe needles and a Luggin capillary, connecting one end of the Luggin capillary with the reference electrode, embedding the other end of the Luggin capillary and the two syringe needles between a bare plant tissue area and an anode of a membrane electrode, and sealing and fixing the embedded Luggin capillary and the embedded syringe needles through silica gel; (5) The circuit connection is that the anode and the cathode of the membrane electrode and the load of an external circuit are connected by titanium wires so that the current generated by the membrane electrode can flow to the load; (6) Microorganism inoculation, namely injecting electrochemical active microorganism strains between the exposed plant tissue area and the anode of the membrane electrode through a syringe needle to inoculate, and sealing the needle by a rubber plug after inoculation; (7) Enhancing microbial activity by injecting a concentration of a solution containing an organic carbon source metabolizable by electrochemically active microorganisms into the anode of the membrane electrode to enhance microbial activity in the anode region; (8) And (3) starting the fuel cell, namely finishing the starting of the fuel cell for generating electricity by the plant and the microorganism after the microorganism inoculation in the step