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CN-120041354-B - Golden camellia plant endophytic bacterium, bacterial agent thereof and application of golden camellia plant endophytic bacterium in resisting influenza A virus

CN120041354BCN 120041354 BCN120041354 BCN 120041354BCN-120041354-B

Abstract

The application provides a golden camellia plant endophytic bacterium, a bacterial agent thereof and application of the golden camellia plant endophytic bacterium in resisting influenza A virus, wherein the golden camellia plant endophytic bacterium is bacillus thuringiensis and is stored in the China general microbiological culture Collection center (CGMCC) with the storage number of 32945. The strain fermentation product has good antiviral activity in-vitro and in-vivo experiments, can effectively inhibit replication of influenza virus, protect infected mice from being injured by lethal influenza virus, and has the effect of regulating immune response, and can enhance secretion of IFN-gamma by downregulating secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving immunity of organisms.

Inventors

  • YAN JIANHUA
  • LI SANMU
  • LU HONGYU
  • XIONG YI

Assignees

  • 广西大学

Dates

Publication Date
20260505
Application Date
20250326

Claims (6)

  1. 1. The golden camellia endophytic bacterium is bacillus thuringiensis (Bacillus thuringiensis) which is stored in the China general microbiological culture Collection center with the preservation number of CGMCC No.32945.
  2. 2. A microbial agent comprising the golden camellia plant endophytic bacterium of claim 1.
  3. 3. Use of the golden camellia endophytic bacterium according to claim 1 or the microbial inoculum according to claim 2 in the preparation of a medicament for inhibiting influenza a virus, The influenza virus is H1N1 or H9N2 influenza virus.
  4. 4. The use of the golden camellia endophytic bacterium according to claim 1 or the microbial inoculum according to claim 2 in the preparation of a medicament or health-care product for enhancing immunity, Through down regulating the secretion of pro-inflammatory factors IL-6 and IL-10, the secretion of IFN-gamma is enhanced, so that the immunity resistance of the organism is improved.
  5. 5. A medicament for inhibiting influenza a virus, characterized in that it comprises a camellia chrysantha plant endophytic bacterium according to claim 1 or a microbial agent according to claim 2.
  6. 6. The medicament of claim 5, further comprising a pharmaceutically acceptable carrier.

Description

Golden camellia plant endophytic bacterium, bacterial agent thereof and application of golden camellia plant endophytic bacterium in resisting influenza A virus The invention field: The application belongs to the field of microorganisms, and particularly provides golden camellia plant endophytic bacteria, a bacterial agent thereof and application of the golden camellia plant endophytic bacteria in resisting influenza A virus. The background technology is as follows: Influenza Virus (IV) is one of the major pathogens responsible for acute respiratory infections, especially in seasonal Influenza and global pandemics, the transmission of which can lead to high morbidity and mortality. Influenza viruses belong to the Orthomyxoviridae (Orthomyxoviridae) family and are characterized by a single-stranded negative-sense RNA genome. Influenza viruses are mainly classified into A, B, C types, wherein influenza a viruses are a research hotspot due to their wide host range, easy variation and cross-species transmission. The influenza A virus can be subjected to rapid variation through antigen drift and antigen conversion, so that the antigenicity of the virus is continuously changed, and great challenges are brought to prevention and control. Variability in influenza virus makes the vaccine and antiviral drugs required each year necessary to be constantly adjusted and updated. Endophytes refer to microorganisms that are present in plants and that generally have a physiological effect on the host plant. Isolation and identification of endophytes is an important research task in microbiology and plant pathology, mainly aimed at screening strains with potential biological activity. Because of its unique biological activity, endophytes become a potential source in antiviral drug development, and a variety of compounds for inhibiting viruses have been isolated from a variety of endophytes. Camellia nitidissima (CAMELLIA NITIDISSIMA) belongs to the family Theaceae, is a special plant in Guangxi and North Vietnam in China, and is called a panda in the plant kingdom because of the rarity. Camellia nitidissima contains various bioactive chemical components such as flavonoids, volatile oils, saponins and various microelements. The components have the pharmacological effects of resisting tumor, resisting oxidation, regulating immunity, resisting inflammation, reducing blood sugar and blood lipid, etc. Researches show that the golden camellia is not only a precious traditional Chinese medicine resource, but also a natural medicine source with huge potential, and particularly has a good application prospect in the antiviral field. Through separating endophytes in camellia chrysantha plant leaf tissues, the camellia chrysantha plant leaf tissue is found to have microorganisms and active substances for resisting influenza viruses, and a new thought is provided for development of antiviral drugs in the future. Disclosure of Invention Endophytes are separated and purified from golden camellia plants, a strain YE10 with remarkable anti-influenza virus activity is screened, and the potential of the strain YE10 against H1N1 and H9N2 subtype influenza viruses is displayed in a cell level and animal model through the supernatant of fermentation liquor. The strain is determined to be bacillus thuringiensis through biochemical identification and 16S rDNA gene sequencing. Experiments show that the fermentation product of the YE10 strain has good antiviral activity in vitro and in vivo experiments, can effectively inhibit the replication of influenza virus, and can protect infected mice from being damaged by lethal influenza virus. In addition, the supernatant of the fermentation broth has the effect of regulating immune response, and can enhance the secretion of IFN-gamma by downregulating the secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving the immunity of the organism. In one aspect, the application provides a golden camellia plant endophytic bacterium which is bacillus thuringiensis (Bacillus thuringiensis) and is stored in the China general microbiological culture Collection center with the preservation number of CGMCC No.32945. In another aspect, the application provides a microbial inoculum comprising the golden camellia endophytic bacteria. The microbial inoculum can be in a liquid or solid form, and can also comprise components such as a carrier, a freeze-drying protective agent, a culture medium and the like besides the golden camellia plant endophytic bacteria. In another aspect, the application provides a ferment, which is the fermentation broth supernatant of the golden camellia endophytic bacteria. Further, the fermented product is prepared by culturing the above golden camellia plant endophytic bacteria strain for 12-36 hours by using a liquid culture medium, collecting a fermentation broth, and taking a supernatant. Further, the liquid culture medium is LB culture medium, and the golden camellia pl