CN-120077822-B - Application method of active microalgae organic fertilizer

CN120077822BCN 120077822 BCN120077822 BCN 120077822BCN-120077822-B

Abstract

The invention discloses an application method of an active microalgae organic fertilizer, which comprises the following steps of adding a water-absorbing adhesion material into microalgae liquid or a mixture of microalgae and bacteria, stirring for a set time to enable the water-absorbing adhesion material to adhere to the microalgae or the mixture of the microalgae and the bacteria, filling seeds into soil, watering, covering the water-absorbing adhesion material adhered to the microalgae or the mixture of the microalgae and the bacteria in a region close to the upper part of the seeds, and simultaneously burying 30% -60% of the water-absorbing adhesion material in the soil, and exposing other parts to air. The method can keep the high activity growth advantage of the microalgae, and can continuously exert the functions of bacteria regulation, fertility slow release, moisture retention and the like.

Inventors

ZHUANG LINLAN
ZHANG JIAN
YANG YUHANG
WANG SHENGNAN

Assignees

山东大学

Dates

Publication Date: 20260508
Application Date: 20250306

Claims (7)

1. The application method of the active microalgae organic fertilizer is characterized by comprising the following steps: Adding a water-absorbing adhesion material into the microalgae liquid or the mixture of microalgae and bacteria, and stirring for a set time to enable the water-absorbing adhesion material to adhere to the microalgae or the mixture of microalgae and bacteria; Burying seeds in soil, watering, covering the area close to the upper part of the seeds with a water-absorbing adhesion material for adhering microalgae or a mixture of microalgae and bacteria, burying 30% -60% of the water-absorbing adhesion material in the soil, and exposing other parts to air; The water-absorbing attaching material is cotton fiber, viscose fiber, bamboo fiber, lyocell or polyvinyl alcohol fiber PVA, and is in a shape of a line, 40-200 strands are in a shape of one strip, and each strip is 2-5 cm.
2. The method for applying an active microalgae organic fertilizer according to claim 1, wherein the microalgae is Chlorella, scenedesmus, haematococcus pluvialis or Dunaliella salina.
3. The method for applying an active microalgae organic fertilizer according to claim 1, wherein the bacteria are rhizobium, azotobacter, phosphorus-dissolving bacteria, mycorrhizal fungi auxiliary bacteria, phytohormone-producing bacteria, biocontrol bacteria, ACC deaminase bacteria or bacteria promoting decomposition of organic matters.
4. The method for applying an active microalgae organic fertilizer according to claim 1, further comprising the steps of culturing and expanding microalgae in a culture medium, wherein the culture medium is a microalgae culture medium rich in nitrogen and phosphorus.
5. The method for applying an active microalgae organic fertilizer according to claim 4, wherein in the microalgae cultivation process, the nitrogen source of the culture solution is KNO 3 , the phosphorus source is NaH 2 PO 4 ,KNO 3 , the concentration is 30-60mg/L, and the concentration of NaH 2 PO 4 is 3-8mg/L.
6. The method for applying an active microalgae organic fertilizer according to claim 4, wherein the microalgae are spread for 3-5 days, the water temperature of the spread culture is above 20 ℃, and the microalgae are stirred regularly or irregularly.
7. The method for applying an active microalgae organic fertilizer according to claim 1, wherein the water-absorbing attachment material is added to the culture medium, and the stirring time is 10-30 min.

Description

Application method of active microalgae organic fertilizer Technical Field The invention belongs to the technical field of organic fertilizer application, and particularly relates to an application method of an active microalgae organic fertilizer. Background The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. Chemical fertilizer application is a common way for solving the problem of lack of nitrogen and phosphorus in soil, but the use amount of chemical fertilizer is difficult to control accurately, and the phenomenon of excessive fertilization is quite common, so that the waste of resources is caused. And secondly, the fertilizer is easy to run off, the solubility of nutrients such as nitrogen, phosphorus and the like in the fertilizer in soil is higher, the nutrients are easy to run off along with rainwater or irrigation water, the fertilization effect is poor, and meanwhile, the running-off fertilizer enters water bodies such as rivers and the like and can cause eutrophication of the water bodies. Finally, the long-term application of the fertilizer can damage the soil aggregate structure, further cause soil hardening, aggravate the decrease of air permeability and water permeability, further influence the activity of soil microorganisms, and reduce the decomposition and accumulation of organic matters. The microalgae fertilizer is environment-friendly, can improve crop productivity and reduce synthetic fertilizer pollution. Among the various types of biofertilizers, formulations based on photosynthetic organisms (including eukaryotic microalgae, anoxic phototrophic organisms and cyanobacteria) are becoming increasingly important for their efficient soil fertility maintenance and plant growth promotion. The research shows that the microalgae can enhance plant immunity, such as blue algae can produce hydrolase and bactericidal substances (benzoic acid and the like), can damage cytoplasmic membranes and inhibit protein synthesis, and has antagonism on various plant pathogens. And secondly, the microalgae contains various organic matters and polysaccharide substances, so that the aggregation of soil particles can be promoted, a good soil structure is formed, the porosity and air permeability of the soil are increased, and the polysaccharide substances can also form a protective film on the surface of the soil particles, so that the evaporation of water is reduced, and the water retention capacity of the soil is improved. In addition, release of microalgae Extracellular Polymers (EPS) provides a rich carbon source, nitrogen source and other prebiotics for microorganisms, regulating the composition of bacterial communities and the function of dissolving phosphorus and fixing nitrogen to promote plant growth. The microalgae fertilizer product takes the algae dry powder as a main form, and is generally mixed with soil before crop planting and used as a base fertilizer. Since microalgae lose part of their biological activity during drying, resulting in their lack of microbial activity in the soil, the nutrient release effect may be inferior to that of fresh algae liquid. Disclosure of Invention Aiming at the defects existing in the prior art, the invention aims to provide an application method of an active microalgae organic fertilizer. The method can keep the high activity growth advantage of the microalgae, and can continuously exert the functions of bacteria regulation, fertility slow release, moisture retention and the like. In order to achieve the above object, the present invention is realized by the following technical scheme: An application method of an active microalgae organic fertilizer comprises the following steps: Adding a water-absorbing adhesion material into the microalgae liquid or the mixture of microalgae and bacteria, and stirring for a set time to enable the water-absorbing adhesion material to adhere to the microalgae or the mixture of microalgae and bacteria; the seeds are buried in the soil, watered, and then the water-absorbing attaching material for attaching microalgae or a mixture of microalgae and bacteria is covered in the area close to the upper part of the seeds, 30% -60% of the water-absorbing attaching material is buried in the soil, and other parts are exposed to the air. The microalgae can continuously grow only by being irradiated on the surface layer of the soil, and can conveniently provide nutrition for the seeds when the microalgae are arranged close to the seeds. In some embodiments, the microalgae is chlorella, scenedesmus, haematococcus pluvialis, or dunaliella salina. In some embodiments, the bacteria are rhizobia, azotobacter, phosphate solubilizing bacteria, mycorrhizal fungi helper bacteria, phytohormone producing bacteria, biocontrol bacteria, ACC deaminase bacteria, or bacteria that promote the breakdown of organic matter. In some embodiments, the method further comprises th