CN-120192884-B - Bacillus megaterium with multiple functions and application thereof

Abstract

The invention belongs to the fields of crop control technology and pesticide residue treatment, and discloses bacillus megatherium with multiple functions and application thereof. The bacillus megatherium is bacillus megatherium (Bacillus megaterium) ZIGUI6588, the preservation number is CCTCC NO: M20242075, the strain can be used for preventing and controlling diseases such as citrus penicillium root-knot nematode and the like, has a function of degrading fruits and soil avermectin, and has a wide application prospect in agricultural production.

Inventors

• Tan Chunliu
• ZHANG CHANGSHUI
• WANG YAPING

Assignees

• 宜昌欣沃裕农业服务有限公司
• 湖北大学

Dates

Publication Date

20260512

Application Date

20250327

Claims (8)

1. 1. An isolated bacillus megaterium (Bacillus megaterium) ZIGUI6588, wherein the preservation number of the bacillus megaterium is CCTCC NO: M20242075.
2. 2. The fermentation broth of bacillus megatherium ZIGUI-6588 of claim 1, wherein the fermentation broth comprises viable bacillus megatherium ZIGUI-6588.
3. 3. A composition comprising bacillus megatherium ZIGUI6588 of claim 1 or the fermentation broth of claim 2.
4. 4. Use of bacillus megatherium ZIGUI6588 of claim 1, a fermentation broth of bacillus megatherium ZIGUI6588 of claim 2, or a composition of claim 3 for controlling citrus diseases, wherein the diseases are diseases caused by Penicillium italicum and/or citrus root knot nematode.
5. 5. The method for controlling the plant according to claim 4, wherein the method comprises the steps of rooting citrus fruits or directly spraying the fermentation broth of Bacillus megaterium ZIGUI6588 of claim 1, bacillus megaterium ZIGUI6588 of claim 2 or the composition of claim 3.
6. 6. Use of bacillus megatherium ZIGUI6588 of claim 1, a fermentation broth of bacillus megatherium ZIGUI6588 of claim 2, or a composition of claim 3 for the preparation of a citrus biocontrol agent, wherein the biocontrol agent has a control target of Penicillium italicum and/or root-knot nematodes.
7. 7. Use of bacillus megatherium ZIGUI6588 of claim 1, a fermentation broth of bacillus megatherium ZIGUI6588 of claim 2, or a composition of claim 3 for degrading avermectin.
8. 8. Use according to claim 7, wherein the purpose of the use is to degrade avermectin on the surface of citrus soil or fruit.

Description

Bacillus megaterium with multiple functions and application thereof Technical Field The invention belongs to the fields of crop control technology and pesticide residue treatment, and particularly relates to bacillus megatherium with multiple functions and application thereof. Background Citrus is also called mandarin orange, etc., is a small arbor of Citrus (Citrus) of Rutaceae (Rutaceae), and is an agricultural prop industry in many areas around the world of Citrus cultivation. Penicilliosis, root knot nematode and the like after citrus harvest are serious diseases of citrus, and seriously threaten the development of the citrus industry. ‌ citrus blue mold ‌ is a disease that primarily damages citrus fruits, and typically occurs on storage and field ripe fruits. The symptoms include softening of the peel, fading of the water stain, and extremely easy breakage by light pressure with hands. Then a plurality of aerial hyphae grow on the center of the surface of the disease spots to form a thick white mold, and the thick white mold rapidly expands into white nearly circular mold spots. Then, the blue or green powder, namely the conidiophore and the conidium, grows from the middle part of the mildew spot. The disease part develops rapidly and can be spread to the whole fruit wet rot within a few days. Citrus root knot nematode is a microorganism which damages plant rhizomes, and can form root nodules with different sizes on root tips of plants, the root nodules are spindle-shaped or irregular, the size of the plant is from nearly sesame grains to mung bean grains, the plant is milk white, and then the plant turns into yellow brown to black brown, and the plant roots are thin and small. ‌ is produced by adopting chemical agents to prevent and treat the two diseases, however, the frequent use of the chemical agents causes the problems of pesticide residue, drug resistance, environmental pollution and the like, and a safer and more effective prevention and control means is needed in production. Biological prevention and control has been widely used in recent years due to the advantages of safety, no residue, difficulty in drug resistance, etc. Avermectin is a common pesticide for controlling crop red spiders, and is widely used in citrus production. Causing a certain pesticide residue in the citrus and soil. The existing antibiotic treatment methods mainly comprise a physical adsorption method, a high-grade oxidation method and a microbial degradation method. The microbial degradation method is a technology favored by researchers because of the continuous enhancement of environmental awareness. Nowadays, reported microorganisms capable of degrading avermectin mainly include bacillus subtilis (Bacillus subtilis), acinetobacter (Acinetobacter lwoffi), shigella (Shigella), white rot fungi and the like, and the microorganisms generally degrade the avermectin by using macrolide degrading enzymes generated by metabolism. Aiming at the production requirement, the invention provides a multifunctional bacillus megatherium which has the functions of preventing and controlling citrus blue mold and root-knot nematode diseases and can degrade abamectin in fruits and soil. Disclosure of Invention The invention aims to provide an isolated bacillus which is bacillus megatherium ZIGUI and 6588 and has a preservation number of CCTCC NO: M20242075. Another object of the invention is to provide the use of bacillus megatherium ZIGUI-6588 in the preparation of microbial agents for citrus cultivation. In order to achieve the above object, the present invention adopts the following technical measures: The applicant separates a bacillus strain from the rhizosphere soil of the citrus plant of the citrus field, and the bacillus strain is identified as bacillus megatherium (Bacillus megaterium) which is sent to the China center for type culture Collection for preservation in the 9 th month of 2024, and is classified and named as Bacillus megaterium ZIGUI6588, wherein the preservation number is CCTCC NO: M20242075, and the place is China, wuhan and university of Wuhan. Bacillus megaterium ZIGUI6588 grew well in LA media colonies, which were pale yellow (FIG. 1). The protection scope of the invention comprises: A fermentation broth of bacillus megatherium ZIGUI6588, said fermentation broth comprising viable bacillus megatherium ZIGUI 6588. A composition comprising bacillus megatherium ZIGUI6588 or a fermentation broth thereof. Use of bacillus megaterium ZIGUI6588, a fermentation broth of bacillus megaterium ZIGUI6588, or a composition comprising bacillus megaterium ZIGUI6588 or a fermentation broth thereof for controlling citrus diseases including citrus blue mold and/or citrus root knot nematode. Preferably, the control method of the application comprises the steps of root irrigation treatment or direct spraying of bacillus megaterium ZIGUI, bacillus megaterium ZIGUI, and bacillus megaterium ZIGUI, and/or bacillus megaterium 6588. Use of bac