CN-120204249-B - Application of hesperetin-7-O-glucoside and derivatives thereof in delaying aging

Abstract

The application relates to application of hesperetin-7-O-glucoside and derivatives thereof in delaying aging. There is provided the use of hesperetin-7-O-glucoside or a pharmaceutically or food acceptable salt thereof, or a tautomer, stereoisomer, enantiomer or non-enantiomer thereof, or a prodrug or metabolite thereof, or a natural source of said hesperetin-7-O-glucoside, salt, isomer, prodrug or metabolite, for the preparation of a composition or formulation for delaying aging and/or prolonging life.

Inventors

• GAN DAN
• ZHANG WANXIANG
• GAO HONG
• ZHANG YAO
• ZENG XINYI
• LI MAN
• XU CANXIN
• XU WEIYI
• CHEN WEIDONG

Assignees

• 仙乐健康科技股份有限公司

Dates

Publication Date

20260508

Application Date

20250422

Claims (15)

1. 1. Use of hesperetin-7-O-glucoside in the preparation of a formulation for prolonging the lifetime.
2. 2. The use according to claim 1, wherein the formulation is for use in humans or animals.
3. 3. The use according to claim 1 or 2, wherein the formulation is in liquid, semi-solid or solid form.
4. 4. The use according to claim 1 or 2, wherein the formulation is in gel, lyophilized form.
5. 5. The use according to claim 1 or 2, wherein the formulation is a medicament.
6. 6. The use according to claim 5, wherein the pharmaceutical is in the form of a powder, tablet, capsule, pill, injection or ointment.
7. 7. The use of claim 5, wherein the formulation further comprises a pharmaceutically acceptable excipient.
8. 8. The use according to claim 1 or 2, wherein the formulation further comprises a pharmaceutically acceptable additive or carrier.
9. 9. Use according to claim 1 or 2, wherein the formulation comprises hesperetin-7-O-glucoside in a unit dose of 0.001-1000 mg.
10. 10. Use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from citrus.
11. 11. Use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from dried orange peel.
12. 12. The use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from one or more of seville orange flower, bitter orange and immature bitter orange.
13. 13. The use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from one or more of grapefruit, lemon, fingered citron, lime, and citron.
14. 14. The use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from exocarpium Citri Grandis.
15. 15. Use according to claim 1 or 2, wherein the hesperetin-7-O-glucoside is derived from orange peel.

Description

Application of hesperetin-7-O-glucoside and derivatives thereof in delaying aging Technical Field The application relates to the field of medicines, in particular to application of hesperetin-7-O-glucoside and derivatives thereof. Background In the most ideal case, the length of life is mainly determined by the aging rate. Senescence is controlled by interrelated processes that occur at the tissue, cellular, molecular and genetic levels. The structure and function of the organism gradually generate irreversible degenerative changes such as protein degradation, tissue atrophy, metabolic rate reduction, calcium metabolism abnormality and the like along with the aging, and diseases caused by aging such as Alzheimer disease, parkinson disease, cardiovascular and cerebrovascular diseases, cancers and the like grow exponentially in the global scope along with the aging. With the continuous increase of population base and specific gravity of the elderly, and the continuous increase of demands of people for resisting aging and prolonging life, the research of human aging mechanism and anti-aging drug development is developed, the human aging speed is delayed within the limit determined by genetic characteristics, and the life of organisms is prolonged, so that the method has important social significance. Caenorhabditis elegans is a classical model organism for carrying out senescence-associated research, and has the advantages of short life cycle, clear genetic background, simple physiological structure, easy culture and the like. Nematodes share many similarities with higher organisms at the cellular and molecular level, such as mechanisms of apoptosis, signal transduction, etc. Most importantly, nematode aging characteristics are obvious, including mobility decline, reproductive capacity decline, body morphological changes (such as muscle atrophy, intestinal tract degeneration and the like) and the like, and the nematode aging characteristics are easy to study, observe and quantify. There is a need in the art to develop substances with life-prolonging effects, which are a viable solution based on caenorhabditis elegans. Disclosure of Invention The present inventors have found for the first time the efficacy of hesperetin-7-O-glucoside and derivatives thereof for prolonging life, as well as the mechanism of action thereof, through the results reflected by caenorhabditis elegans. The nematode for the anti-aging model has the following advantages that 60% -80% of genes of the nematode are highly conserved with related genes of human beings, and the nematode has 12 signal transduction pathways discovered so far, so the application utilizes caenorhabditis elegans as model organisms for verifying anti-aging drugs. The abundant genetic resources of nematodes can be utilized, proper mutants are selected according to the purpose of research, and the mechanisms of senescence and aging are studied, and in essence, several theories of senescence mechanism are proved in nematodes. Thus, drugs having anti-aging effects on insects are generally considered to have the same efficacy as humans. Caenorhabditis elegans has been used for life analysis and has been 30 years old. And the method is a first-push model for aging research due to the unique advantages. The nematode generation cycle is short, usually about 3 days, and the life span is short, usually about 3 weeks. This makes possible the repeated stability of the experiment. In order to ensure the reliability of the experimental method and the accuracy of the experimental result, and the more accurate and reliable information obtained by drug screening, repeated experiments are necessary. The caenorhabditis elegans has the unique advantages, and becomes a first-push model for aging research. Thus nematodes can be used to evaluate the anti-aging effect of the composition and further judge that the composition is being used for the preparation of anti-aging medicaments. Nematode knock-down mutant strain aak-2 (gt 33) represents the AMPK signaling pathway, AMPK is a protein kinase that is widely found in humans and other organisms and has the main function of regulating cellular energy metabolism and maintaining energy balance. It can activate or inhibit multiple cellular metabolic pathways by phosphorylating a range of substrate proteins. The activation of AMPK can delay the aging process by regulating the mechanisms of cell energy metabolism, inhibiting oxidative stress, inflammatory reaction and the like, and has important anti-aging significance. vha-3 (ok 1501) insect strain represents lysosomal acidification and lysosomal autophagy pathway, and vha-3 expression reduces damage to V-ATPase proton pump on lysosomal membrane, affecting lysosomal acidification and autophagy function. rsks-1 (ok 1255) insect strain represents the TOR signaling pathway, RSKS-1 is the only nematode homolog of human S6-kinase (S6K), playing a key role in protein synthesis, longevity, autophagy an