CN-120230862-B - SNP (Single nucleotide polymorphism) marker related to Duroc pig variety identification and application thereof

Abstract

The invention relates to an SNP marker related to Duroc pig variety identification and application thereof. The molecular marker contains a sequence shown as SEQ ID NO.1, wherein the sequence contains an rs327637000 nucleotide locus at 44090690bp of chromosome 8 of an international pig genome 11.1 version reference sequence, the polymorphism at the locus is G/T, the TT genotype is Duroc pig, the TG genotype is Dulong ternary pig, and the GG genotype is long binary pig. The SNP marker provided by the invention can simply and efficiently identify Duroc, dulong ternary pigs and long binary pigs, and is convenient for breeders to trace the breeding background. The invention provides a new technical means for pig variety identification, and has important application value and social benefit.

Inventors

• DING XIANGDONG
• ZHANG ZIPENG
• LIN CHUAN
• SHAO YURU
• Fang Zhengwen

Assignees

• 中国农业大学
• 石家庄博瑞迪生物技术有限公司

Dates

Publication Date

20260508

Application Date

20250212

Claims (3)

1. 1. The application of a primer pair for detecting SNP molecular markers in identifying Duroc pig breeds is characterized in that the SNP molecular markers are rs327637000 nucleotide sites positioned at 44090690bp of international pig genome 11.1 version reference sequence 8 chromosome, the application is that genomic DNA is extracted from pigs to be detected, the extracted genomic DNA is diluted and used as a DNA template, the primer pair is used for carrying out PCR amplification on the DNA template and judging the typing of the SNP molecular markers, TT genotypes are Duroc pigs, TG genotypes are Dulong ternary pigs, and GG genotypes are long binary pigs.
2. 2. The application of the primer pair for detecting SNP molecular markers in identifying Duroc pig breeds according to claim 1, wherein the nucleotide sequence of the forward primer for detecting the Alle T is shown as SEQ ID NO.4, the nucleotide sequence of the forward primer for detecting the Alle G is shown as SEQ ID NO.5, and the nucleotide sequence of the universal reverse primer is shown as SEQ ID NO. 6.
3. 3. The application of the kit for detecting the SNP molecular marker in identifying the Duroc pig breeds is characterized in that the kit comprises the primer pair of claim 2, the SNP molecular marker is an rs327637000 nucleotide locus positioned at 44090690bp of chromosome 8 of international pig genome 11.1 version reference sequence, the application is that genomic DNA is extracted from a pig to be detected, the extracted genomic DNA is diluted and used as a DNA template, the primer pair is used for carrying out PCR amplification on the DNA template and judging the typing of the SNP molecular marker, the TT genotype is Duroc pigs, the TG genotype is Dulong and large three-way pigs, and the GG genotype is long and large two-way pigs.

Description

SNP (Single nucleotide polymorphism) marker related to Duroc pig variety identification and application thereof Technical Field The invention relates to the fields of molecular biology and genetic breeding, in particular to an SNP marker related to Duroc pig variety identification and application thereof. Background The pig species widely raised in the livestock industry currently include Duroc, dulong and large ternary pigs (namely Duroc, long and large white pigs three-way hybridization offspring) and long and large binary pigs (namely long and large white pigs two-way hybridization offspring), wherein the Duroc pigs are known for the advantages of high growth speed, strong constitution, strong stress resistance and the like, and are widely used as terminal male parents in commercial pig breeding systems in China. In animal husbandry, pigs of different varieties have remarkable differences in growth speed, meat quality, reproductive capacity and the like, so that accurately distinguishing the varieties of the pigs has great significance in formulating scientific breeding strategies, optimizing breeding structures and improving economic benefits. Secondly, duroc, duchet and Duchet are taken as important economic pig breeds, and take up important roles in the pig industry, however, with the expansion of the breeding scale and the popularization of interspecies cross breeding, the situations of error and even loss of pedigree record often occur in production. The pig variety identification is a key link for ensuring the breeding benefit and genetic stability, and the traditional pig variety identification method mainly depends on morphological characteristics and physiological indexes such as body type, hair color, ear type, growth speed and the like, however, the methods are easily influenced by various factors such as environment, feeding condition, growth and development stage and the like, so that the identification result is inaccurate and stable. In addition, for young pigs or swine breeds with similar appearance characteristics, traditional methods tend to be difficult to distinguish effectively. Misjudgment slows down the genetic progress of the population, bringing greater breeding loss than loss of pedigree. Therefore, aiming at widely-raised pigs, it is important to develop an accurate, stable and efficient pig variety identification method. With the rapid development of molecular biology technology, a molecular marker technology based on Single Nucleotide Polymorphism (SNP) is gradually becoming a new means for identifying pig breeds. SNP refers to DNA sequence polymorphism caused by variation of single nucleotide at genome level, and has the advantages of large quantity, wide distribution, good genetic stability and the like. By detecting the genotype of a specific SNP locus, the accurate identification of the pig variety can be realized. In the pig genome, there are SNP loci associated with the breeds, and there are significant genotype differences between different breeds. The SNP marker provides a new technical means for pig variety identification, and has important application value and social benefit. The prior researches do not find SNP loci with completely different genotypes in Duroc, dulong and large ternary pigs and long and large binary pigs, so that variety identification can be performed only through a large number (more than 1000) of SNP loci with certain differences combined with a statistical model. Therefore, there is a need to develop SNP markers and identification methods thereof related to identification of Duroc, dulong ternary pigs and long binary pigs. Disclosure of Invention In order to solve the problems, the invention provides SNP markers related to Duroc pig variety identification and application thereof. The invention provides a SNP molecular marker related to Duroc pig variety identification, the molecular marker contains a sequence shown as SEQ ID NO.1, the sequence contains an rs327637000 nucleotide locus at 44090690bp of chromosome 8 of international pig genome 11.1 version reference sequence, the polymorphism at the locus is G/T, wherein TT genotype is Duroc pig, TG genotype is Dulong and large ternary pig, and GG genotype is long and large binary pig. The invention also provides a PCR primer pair for detecting the SNP molecular marker, which comprises a forward primer with a nucleotide sequence shown as SEQ ID NO.2 and a reverse primer with a nucleotide sequence shown as SEQ ID NO. 3. The invention also provides a KASP primer pair for detecting the SNP molecular marker, wherein the nucleotide sequence of a forward primer for detecting the Alle T is shown as SEQ ID NO.4, the nucleotide sequence of a forward primer for detecting the Alle G is shown as SEQ ID NO.5, and the nucleotide sequence of a universal reverse primer is shown as SEQ ID NO. 6. The invention also provides a kit for detecting the SNP molecular marker, which comprises the primer pair as set forth