Search

CN-120366015-B - Cell culture device convenient to change culture solution

CN120366015BCN 120366015 BCN120366015 BCN 120366015BCN-120366015-B

Abstract

The invention provides a cell culture device convenient for replacing culture solution, which relates to the technical field of cell culture and comprises a main constant temperature box body, an upper communication cover, a middle liquid supply cover plate and a drying box, wherein the upper communication cover is arranged in the main constant temperature box body, the middle liquid supply cover plate is arranged at the upper part of the upper communication cover in a circumferential rotation manner, the drying box is fixedly arranged at the outer side of the main constant temperature box body, a lower air guide inclined surface with a conical structure shrinking towards the center is arranged at the lower part of the middle liquid supply cover plate, a lower air guide plate is arranged on the lower air guide inclined surface in an annular array manner, and a first liquid supply pipe and a second liquid supply pipe are arranged at the bottom of the lower air guide inclined surface in a penetrating manner. When the dry air flows through the lower culture dish, evaporation of redundant moisture in the culture solution is accelerated, the problem of nutrient solution dilution caused by moisture accumulation is avoided, and the problem of additional loss of microorganisms caused by synchronously extracting the cultured microorganisms when the redundant water solution is mechanically extracted is solved.

Inventors

  • DONG JUNYI
  • WANG XIANG
  • WANG MIN
  • LI YUE

Assignees

  • 兰立生物科技(苏州)有限公司

Dates

Publication Date
20260508
Application Date
20250325

Claims (5)

  1. 1. A cell culture device convenient for replacing culture solution comprises a main constant temperature box body (1), an upper communication cover (2), a middle liquid supply cover plate (3) and a drying box (4), and is characterized in that the upper communication cover (2) is arranged in the main constant temperature box body (1), the middle liquid supply cover plate (3) is arranged on the upper circumference of the upper communication cover (2) in a rotating way, the drying box (4) is fixedly arranged on the outer side of the main constant temperature box body (1), a lower air guide inclined surface (301) with a conical structure shrinking towards the center is arranged on the lower part of the middle liquid supply cover plate (3), a lower air guide plate (302) is arranged on the lower air guide inclined surface (301) in an annular array mode, a first liquid supply pipe (303) and a second liquid supply pipe (304) are arranged at the bottom of the lower air guide inclined surface in a penetrating way, the upper ends of the first liquid supply pipe (303) and the second liquid supply pipe (304) are in hose communication with an upper connecting branch pipe (305), the upper ends of the upper connecting branch pipe (305) and the upper connecting liquid storage pipe (306) are in circumference rotating and are kept in communication, the middle constant temperature box body (1) is matched with a main constant temperature box door (103) and a main tube (104), a liquid pump (104) is arranged on the middle constant temperature box body (1) and a main constant temperature box (104) is arranged on the main constant temperature box body (1), one side intercommunication of going up intercommunication lid (2) is provided with air inlet (201), the opposite side intercommunication of going up intercommunication lid (2) is provided with gas outlet (202), the fixed driving motor (203) that is provided with in top of going up intercommunication lid (2), the bottom of air inlet (201), gas outlet (202) is all fixed to be provided with down and connects slide post (204), the lower part circumference of going up intercommunication lid (2) is rotated and is provided with down screwed ring (206), lower screwed ring (206) lower part and lower culture dish (205) screw-thread fit connection, down wind-guiding inclined plane (301), lower aviation baffle (302), first feed pipe (303), second feed pipe (304) are in the inboard of going up intercommunication lid (2) and are in the top of lower culture dish (205), down wind-guiding inclined plane (301), lower aviation baffle (302) are in between air inlet (201), gas outlet (202), feed apron (3) in the through gear engagement of last driving motor (203) main shaft.
  2. 2. A cell culture apparatus for facilitating replacement of a culture medium according to claim 1, The lower connecting sliding column (204) is vertically and slidably connected with the main constant temperature box body (1), the upper communicating cover (2) and the lower culture dish (205) are both positioned in a closed space formed by closing the main constant temperature box body (1) and the movable box door (101), the air outlet (202) is in hose communication with the ventilation fan (102), and the air outlet (202) is in unidirectional communication with the ventilation fan (102) through a one-way valve.
  3. 3. A cell culture apparatus for facilitating replacement of a culture medium according to claim 1, The first liquid supply pipe (303) is positioned in the middle of the lower air guide inclined plane (301), the second liquid supply pipe (304) is positioned on the outer side of the first liquid supply pipe (303), the upper connecting main pipe (306) is fixed with the top of the main constant temperature box body (1), and the upper end of the upper connecting main pipe (306) is communicated with the liquid feeding pump (103).
  4. 4. A cell culture apparatus for facilitating replacement of a culture medium according to claim 1, The drying box is characterized in that two sides of the drying box (4) are respectively communicated with an air inlet pipe (401) and an air outlet pipe (402), a middle baffle (403) is fixedly arranged on the inner side of the drying box (4), and a drying agent (404) is arranged on the outer side of the middle baffle (403) in a surrounding mode.
  5. 5. A cell culture apparatus according to claim 4, wherein the cell culture apparatus is capable of facilitating replacement of the culture medium, The middle baffle plate (403) and the drying agent (404) are arranged between the air inlet pipe (401) and the air outlet pipe (402), the air outlet pipe (402) is in hose communication with the air inlet (201), and the air inlet pipe (401) is in hose communication with the air outlet pipe (402) of the other drying box (4).

Description

Cell culture device convenient to change culture solution Technical Field The invention relates to the technical field of cell culture equipment, in particular to a cell culture device convenient for replacing culture solution. Background The cell culture device provides a constant-temperature controllable living culture environment for microorganisms (such as viruses, bacteria and fungi) and helps to separate and amplify pathogens from clinical samples, for example, bacteria in sputum of a patient are cultured through the cell culture device, infection types are screened, drug sensitivity is detected, and diagnosis is carried out, so that analysis and symptomatic treatment on conditions are realized. Microorganisms absorb nutrients through the culture solution in the culture dish, the culture solution of the microorganisms needs to be added periodically, the culture solution is composed of various nutrients and water, the conventional cell culture device can be added for many times after the nutrient components of the culture solution are consumed, the water consumption in the nutrient solution is relatively slow, so that the water solution is accumulated more, the later added nutrient solution can be diluted by the accumulation of the water solution, and the cultured microorganisms can be synchronously extracted when the redundant water solution is extracted mechanically, so that the additional loss of the microorganisms is caused. Disclosure of Invention The embodiment of the disclosure relates to a cell culture device convenient to change culture solution, through two drying cabinet series connection structure, physical adsorption through the drier effect carries out the drying operation of air, the evaporation of unnecessary water liquid in the lower culture dish is accelerated to dry air, when the dry air flows through the lower culture dish, the evaporation of unnecessary moisture in the culture solution has been accelerated, the problem of nutrient solution dilution because of the moisture accumulation has been avoided, simultaneously, this design has avoided the microorganism loss that causes through mechanical extraction water time-sharing in traditional method, through the test, cell survival rate can improve 15% -20%, the culture solution change cycle is prolonged more than 30%, first liquid supply pipe, the scope of second liquid supply pipe circumferential rotation enlarges the nutrient solution, realize the even filling of nutrient solution in the lower culture dish, promote the evenly distributed of nutrient solution, guarantee the nutrition even supply and the cultivation work of like virus, bacterium, fungi in the lower culture dish, make nutrient solution concentration homogeneity in the culture dish promote more than 98%. The first aspect of the disclosure provides a cell culture device convenient for replacing culture solution, which specifically comprises a main constant temperature box body, an upper communication cover, a middle liquid supply cover plate and a drying box, wherein the upper communication cover is arranged in the main constant temperature box body, the middle liquid supply cover plate is arranged on the upper part of the upper communication cover in a circumferential rotation manner, the drying box is fixedly arranged on the outer side of the main constant temperature box body, a movable box door is arranged in the middle of the main constant temperature box body in a matched rotation manner through a hinge, a ventilation fan is fixedly arranged in the middle of the main constant temperature box body, a liquid feeding pump and a liquid storage box are fixedly arranged at the top of the main constant temperature box body, and the liquid feeding pump and the liquid storage box are communicated. In at least some embodiments, one side of the upper communication cover is communicated with an air inlet, the other side of the upper communication cover is communicated with an air outlet, the top of the upper communication cover is fixedly provided with an upper driving motor, the bottoms of the air inlet and the air outlet are fixedly provided with a lower connecting sliding column, the lower part of the upper communication cover is circumferentially rotated and provided with a lower threaded ring, and the lower part of the lower threaded ring is in threaded fit connection with a lower culture dish. In at least some embodiments, the lower connecting slide column is vertically and slidably connected with the main constant temperature box body, the upper communicating cover and the lower culture dish are both positioned in a closed space formed by the main constant temperature box body and the movable box door, the air outlet is communicated with the flexible pipe of the ventilating fan, the air outlet is communicated with the ventilating fan in one way through the one-way valve, the air inside the ventilating fan cannot flow towards the air outlet, the ventilating fan is commun