CN-120400240-B - Application of GsMYB gene in low-phosphorus stress tolerance of plants

Abstract

The invention relates to the technical field of genetic engineering, in particular to a plant low phosphorus resistant important gene GsMYB and application thereof. Researches show that GsMYB gene is up-regulated under the induction of low-phosphorus stress, under the treatment condition of different phosphorus concentrations, the over-expression GsMYB can obviously increase the biomass of transgenic plants so as to promote the growth of the plants under the low-phosphorus condition, and meanwhile, the over-expression GsMYB10 can improve the tolerance of the plants to the low-phosphorus stress capability and reduce the inhibition of low-phosphorus on the growth of plant root systems.

Inventors

• MA QIBIN
• DU PENGXIN
• YANG CE
• LIANG ZHONGYI
• LU XIANG
• NIAN HAI
• CAI ZHANDONG
• CHENG YANBO

Assignees

• 华南农业大学

Dates

Publication Date

20260512

Application Date

20250430

Claims (1)

1. 1. The application of the over-expression gene GsMYB in improving the low-phosphorus stress resistance of soybean is characterized in that the nucleotide sequence of the gene GsMYB10 is shown as SEQ ID NO. 1; The specific steps of the application are that a gene GsMYB shown in SEQ ID NO.1 is introduced into acceptor soybean to obtain transgenic soybean, and the gene GsMYB codes an amino acid sequence shown in SEQ ID NO. 2; the improvement in the low phosphorus stress tolerance is manifested by an increase in the root length or lateral root number thereof.

Description

Application of GsMYB gene in low-phosphorus stress tolerance of plants Technical Field The invention relates to the technical field of genetic engineering, in particular to application of GsMYB gene in low-phosphorus stress resistance of plants. Background The soybean is a plant with rich nutrition, the protein content of the soybean is 2.5-8 times of that of cereal and potato foods, and in addition, the fat, calcium, phosphorus, iron, vitamin B 1, vitamin B 2 and other human essential substances are higher than those of cereal and potato foods. The use of soybeans is very widespread. In the aspect of eating, the soybeans can be processed into bean products such as bean curd, soybean milk, dried beancurd sticks and the like. The fermented bean products include fermented bean curd, soybean paste, soy sauce, fermented soybean, natto, etc., while the non-fermented bean products include watery bean curd, dried bean curd, bean sprouts, marinated bean products, etc. In the aspect of feeding, the stems, leaves and bean dregs of the soybeans are good fertilizers and feeds, and are suitable for ruminant livestock such as chickens, pigs, cows and the like. Soybeans are phosphorus-loving crops, phosphorus plays a key role in the whole life cycle of the soybeans, and if the soybeans cannot absorb enough phosphorus, the growth of root nodules of the soybeans can be inhibited, the nitrogen fixation amount is reduced, and finally the yield and quality of the soybeans are seriously reduced. China is the largest soybean consuming country worldwide, with annual demand exceeding 1 million tons. However, the self-sufficiency rate of chinese soybean is only 18% -20%. The Chinese soybeans are mainly produced in northeast areas, and the yield is about 50-60% of the total yield of the whole country. While the Huang-Huai-Hai region and the south red soil region have rich land resources, the growth of soybean plants is limited and the pod number is reduced due to the lack of soil phosphorus, so that the production and quality of soybeans are finally affected. Therefore, screening out the high-efficiency phosphorus genes and improving the low phosphorus resistance of soybean is one of important ways for improving the yield and quality of soybean. Disclosure of Invention In order to solve the above-mentioned problems in the background art, an object of the present invention is to provide an application of gene GsMYB to increase the low-phosphorus stress tolerance of plants, to increase the yield and quality of soybeans in low-phosphorus environments, wherein the nucleotide sequence of gene GsMYB10 is shown as SEQ ID NO.1, or the nucleotide sequence of gene GsMYB is shown as SEQ ID NO. 2. The application improves the low phosphorus stress resistance of plants by over-expressing the gene GsMYB in the plants. Further, the plant is soybean. The invention also provides a method for improving the low-phosphorus stress resistance of plants, which is used for improving the expression level of an amino acid sequence shown as SEQ ID NO.2 in a receptor plant, and comprises the specific steps of introducing a gene GsMYB10 shown as SEQ ID NO.1 into the receptor plant to obtain a transgenic soybean plant, wherein the low-phosphorus stress resistance of the transgenic soybean plant is higher than that of the receptor plant, or the amino acid sequence shown as SEQ ID NO.2 is over-expressed in the receptor plant. Further, the plant's increased tolerance to low phosphorus stress is manifested by an increase in its root length or lateral root number. The invention aims at providing an application of recombinant plasmid, recombinant vector and transgenic plant cell line of gene GsMYB10 in improving low-phosphorus stress tolerance of plants, wherein the nucleotide sequence of gene GsMYB10 is shown as SEQ ID NO.1, or the gene GsMYB encodes an amino acid sequence shown as SEQ ID NO. 2. The application relates to a method for researching the growth of soybean low-phosphorus-resistant gene GsMYB in low-phosphorus stress environment, which comprises the following specific steps: The soybean is used as a material, the agrobacterium-mediated soybean cotyledonary node transformation method is utilized, the GsMYB gene is genetically transformed into the soybean by using the agrobacterium tumefaciens-mediated soybean cotyledonary node method, a soybean plant with over-expressed GsMYB gene is obtained, and the function and application potential of the GsMYB gene in the aspect of improving the low-phosphorus tolerance of the soybean are verified by comparing indexes such as growth performance, phosphorus absorption efficiency, root system development and the like of the transgenic plant and a wild plant under the condition of low-phosphorus stress. In conclusion, the invention has the beneficial effects that through cloning and analysis of the soybean gene GsMYB, the agrobacterium-mediated soybean cotyledonary node transformation method is adopted, the GsMYB gene can