CN-120442475-B - Pantoea agglomerans RMM02 and application of product thereof in preventing and controlling false smut of rice

Abstract

The invention discloses Pantoea agglomerans RMM02 and application of a product thereof in preventing and controlling false smut of rice. The classification of the pantoea agglomerans RMM02 is named Pantoea agglomerans, the preservation number is GDMCC No.65516, the preservation unit is the Guangdong province microorganism strain preservation center, and the preservation time is 2024, 11, 29 days. The pantoea agglomerans RMM02 is separated from the soil of a plot where the false smut of rice occurs, and is obtained by screening through an indoor counter test, and has remarkable inhibitory activity on the false smut of rice. Indoor antibacterial tests show that the active bacteria have remarkable inhibitory activity on the false smut, and indoor and field tests show that the active bacteria have remarkable inhibitory effect on the growth of the false smut and can control the development of the disease during the disease period of the false smut in the field. The biocontrol microbial inoculum prepared by the fermentation of the strain can effectively prevent and control the diseases and the pests of the rice false smut and ensure the high and excellent yield of the rice.

Inventors

• CHEN LIEZHONG
• HOU JIAYIN
• LI MENG
• LIU ZHIYU

Assignees

• 浙江省农业科学院

Dates

Publication Date

20260512

Application Date

20250512

Claims (5)

1. 1. A biocontrol microbial agent is characterized by comprising pantoea agglomerans RMM02 or fermentation liquor of pantoea agglomerans RMM02, wherein the number of viable bacteria of pantoea agglomerans RMM02 in the biocontrol microbial agent is 1 multiplied by 10 5 cfu/mL-1×10 10 cfu/mL, the classification name of pantoea agglomerans RMM02 is Pantoea agglomerans, the preservation number is GDMCC No:65516, the preservation unit is the Guangdong province microorganism strain preservation center, and the preservation time is 2024, 11 months and 29 days; the protective agent is one or more of carboxymethyl cellulose, starch, chitosan and lignin; The concentration of the protective agent in the biocontrol microbial agent is 1-10% m/m.
2. 2. The use of the biocontrol microbial inoculum according to claim 1 for preparing products for preventing and treating false smut of rice.
3. 3. The method for preparing the biocontrol microbial agent of claim 1, which is characterized by comprising the following steps: ① Inoculating Pantoea agglomerans RMM02 into LB liquid culture medium, and placing into a constant temperature shaking table for shaking fermentation at 26-33 ℃ for 24-60h to obtain fermentation liquor; ② And adding a protective agent and sterile water, and regulating the concentration of the cultured fermentation broth to be 1 multiplied by 10 5 cfu/mL-1×10 10 cfu/mL.
4. 4. A method for preventing and controlling false smut of rice, which is characterized by comprising the following steps: Spraying the biocontrol microbial inoculum of claim 1 or prepared according to the method of claim 3 10-15 days before the break of rice, wherein the spraying concentration is 1×10 5 cfu/mL-1×10 7 cfu/mL, and if rainfall is encountered within 4 hours after the application, the biocontrol microbial inoculum is applied again with the same spraying concentration after rain stop.
5. 5. A method for preventing and controlling false smut of rice, which is characterized by comprising the following steps: Spraying the biocontrol microbial inoculum of claim 1 or prepared by the method of claim 30-5 days before breaking the rice or 0-5 days after heading, wherein the spraying concentration is 1X 10 8 cfu/mL-1×10 10 cfu/mL, if rainfall is encountered within 4 hours after the spraying, the same spraying concentration is used for supplementing once after raining, and the same spraying concentration is used for supplementing once after 3 days after the first spraying.

Description

Pantoea agglomerans RMM02 and application of product thereof in preventing and controlling false smut of rice Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to Pantoea agglomerans RMM02 and application of a product thereof in preventing and treating false smut of rice. Background The false smut is a rice disease caused by false smut germ (Ustilaginoidea virens) and is widely distributed in all large rice producing areas worldwide. The ustilaginoidea virens mainly invades in the flowering phase of rice, develops symptoms in the grouting phase and becomes disaster in the mature phase. At present, the false smut mainly still depends on chemical prevention and control, but the false smut is still inevitably transmitted and retransmitted in actual production due to the short prevention and control window period (5-7 days before the break of rice) and the weather influence of rain and the like. In order to improve the effective control of false smut, farmers use chemical pesticides for multiple times, and increase the production cost and the risk of pollution of rice and environmental pesticide residues. The active microbial biocontrol microbial inoculum is an ideal choice for replacing chemical pesticides due to the advantages of environmental safety, no public hazard, no residue and the like. In view of the common and repeated sending and the repeated sending of the ustilaginoidea virens, the development of more safe and effective biocontrol bactericides has great application significance. Disclosure of Invention The invention aims to provide Pantoea agglomerans RMM02 and application of a product thereof in preventing and treating false smut of rice. In order to solve the problems existing in the prior art, the invention adopts the following technical scheme: In a first aspect, the invention provides a pantoea agglomerans RMM02, wherein the class name of the pantoea agglomerans RMM02 is Pantoea agglomerans, the preservation number is GDMCC No.65516, the preservation unit is the microorganism strain preservation center of Guangdong province, and the preservation time is 2024, 11 months and 29 days. In a second aspect, the invention provides a microbial inoculum comprising the fermentation broth of Pantoea agglomerans RMM02 of the first aspect. In a third aspect, the invention provides an application of the pantoea agglomerans RMM02 described in the first aspect or the microbial inoculum described in the second aspect in preparing a product for preventing and treating false smut of rice. Further, the product is a biological antibacterial agent. In a fourth aspect, the invention provides a biocontrol microbial agent, wherein the biocontrol microbial agent comprises the pantoea agglomerans RMM02 in the first aspect and/or the microbial agent in the second aspect, and the number of viable bacteria in the biocontrol microbial agent is not less than 1×10 5 cfu/mL. Preferably, the number of viable bacteria in the biocontrol microbial inoculum is 1X 10 5cfu/mL-1×1010 cfu/mL. Further preferably, the number of viable bacteria in the biocontrol agent is 1X 10 9cfu/mL-1×1010 cfu/mL, such as 1×109cfu/mL、2×109cfu/mL、3×109cfu/mL、4×109cfu/mL、5×109cfu/mL、 6X 10 9cfu/mL、7×109cfu/mL、8×109cfu/mL、9×109cfu/mL、1×1010 cfu/mL, etc., other point values within this range of values may be selected. Further, the biocontrol microbial agent also comprises a protective agent and sterile water, wherein the protective agent is one or more of carboxymethyl cellulose, methyl cellulose, starch, cyclodextrin, chitosan and lignin, and the concentration of the protective agent in the biocontrol microbial agent is 1-10% (m/m). In a fifth aspect, the present invention also provides a method for preparing the biocontrol microbial agent according to the fourth aspect, which comprises the following steps: ① Inoculating Pantoea agglomerans RMM02 into LB liquid culture medium, and placing into a constant temperature shaking table for shaking fermentation at 26-33 ℃ for 24-60h to obtain fermentation liquor; ② And adding a protective agent solution, and regulating the concentration of the cultured fermentation broth to be 1 multiplied by 10 5cfu/mL-1×1010 cfu/mL. Further, the OD value of the fermentation broth prepared in the step ① is more than or equal to 0.8 under the condition of 600 nm. In a sixth aspect, the present invention provides a method for controlling false smut of rice, comprising the steps of: Spraying the biocontrol microbial agent in the fourth aspect or the biocontrol microbial agent prepared according to the method in the fifth aspect 15 days before the break of the rice and 5 days after the heading, wherein the spraying concentration of the biocontrol microbial agent is 1X 10 5cfu/mL-1×1010 cfu/mL. If rainfall is encountered within 4 hours after the application, the spraying concentration is the same after the rain stop, and the spraying concentration is used for one time. Further,